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Expression patterns and antifungal function study of KaSPI in Mythimna separata

Published online by Cambridge University Press:  21 September 2023

Ya-Ru Chen
Affiliation:
College of Plant Protection, Northeast Agricultural University, Harbin 150030, China CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Hong-Jia Yang
Affiliation:
College of Plant Protection, Northeast Agricultural University, Harbin 150030, China
Jin-Myong Cha
Affiliation:
College of Plant Protection, Northeast Agricultural University, Harbin 150030, China Kyeungsang Sariwon Agricultural University, Pyong Yang 95003, DPR of Korea
Xin-Xin Zhang*
Affiliation:
College of Plant Protection, Northeast Agricultural University, Harbin 150030, China
Dong Fan*
Affiliation:
College of Plant Protection, Northeast Agricultural University, Harbin 150030, China
*
Corresponding author: Xin-Xin Zhang; Email: xinxinz@neau.edu.cn; Dong Fan; Email: dnfd@163.com
Corresponding author: Xin-Xin Zhang; Email: xinxinz@neau.edu.cn; Dong Fan; Email: dnfd@163.com

Abstract

Kazal-type serine protease inhibitors (KaSPI) play important roles in insect growth, development, digestion, metabolism and immune defence. In this study, based on the transcriptome of Mythimna separata, the cDNA sequence of MsKaSPI with Kazal domain was uploaded to GenBank (MN931651). Spatial and temporal expression analysis showed that MsKaSPI was expressed at different developmental stages and different tissues, and it was induced by 20-hydroxyecdysone in third-instar larvae of M. separata. After 24 h infection by Beauveria bassiana, the expression level of MsKaSPI and the corresponding MsKaSPI content were significantly up-regulated, being 6.42-fold and 1.91-fold to the control group, respectively, while the activities of serine protease, trypsin and chymotrypsin were inhibited. After RNA interference interfered with MsKaSPI for 6 h, the expression decreased by 73.44%, the corresponding content of MsKaSPI protein decreased by 55.66% after 12 h, and the activities of serine protease and trypsin were significantly enhanced. Meanwhile, both the larval and pupal stages of M. separata were prolonged, the weights were reduced and the number of eggs per female decreased by 181. Beauveria bassiana infection also increased the mortality of MsKaSPI-silenced M. separata by 18.96%. These prove MsKaSPI can not only result in slow growth and low fecundity of M. separata by regulating the activity of related protease, but also participate in the resistance to pathogenic fungi by regulating the serine protease inhibitor content and the activities of related serine protease.

Type
Research Paper
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press

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