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Study of magnesium bioavailability using stable isotopes and the inductively-coupled plasma mass spectrometry technique in the rat: single and double labelling approaches

Published online by Cambridge University Press:  09 March 2007

Charles Coudray ,
Denise Pepin ,
Jean Claude Tressol ,
Jacques Bellanger  and
Yves Rayssiguier
Charles Coudray
Affiliation:
Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Theix/Clermont-Ferrand, 63122 Saint Genès Champanelle, France
Denise Pepin
Affiliation:
Laboratoire d'hydrologie, Institut Louise Blanquet, Faculté de Pharmucie, Université d'Auvergne, 63000 Clermont-Ferrand, France
Jean Claude Tressol
Affiliation:
Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Theix/Clermont-Ferrand, 63122 Saint Genès Champanelle, France
Jacques Bellanger
Affiliation:
Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Theix/Clermont-Ferrand, 63122 Saint Genès Champanelle, France
Yves Rayssiguier
Affiliation:
Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Theix/Clermont-Ferrand, 63122 Saint Genès Champanelle, France
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Abstract

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The present work aimed to investigate the feasibility of using stable isotopes and inductively-coupled plasma mass spectrometry (ICP/MS) to study Mg absorption in rats. Male Wistar rats, aged 7 weeks and weighing 180g, were used. They were fed on a semi-purified diet containing 1070mg Mg/kg, and had free access to feed and distilled water. In the first experiment, after a 16d adaptation period, two doses of enriched 25Mg (6 and 12mg) were administered by oral intubation, faeces and urine were collected daily and blood was sampled. Isotope ratios were determined by ICP/;MS. ‘True’ absorption values, using the faecal isotope data, were 0.63 and 0.56 in rats receiving 6 and 12mg 25Mg respectively, while apparent absorption was 0.50 for two successive periods of metabolic balance studies. Moreover, the oral isotope administration resulted in a measurable isotopic enrichment in plasma within hours which was still detectable on the third day following the isotope administration. In the second experiment, investigating the double labelling technique, similar rats were dosed Simultaneously with 5mg 26Mg orally (premixed with diet) and 0.29mg 25Mg intravenously. The calculated Mg true absorption values were very similar when calculated from blood or urine data (0.38) but were lower than that obtained from faecal data (0.50). The possible causes of such an unexpected difference and limits of the double labelling technique for Mg absorption are discussed here. Together these results indicate that although 25Mg and 26Mg isotopes have high natural abundance, the described methodology permits meaningful investigations of Mg bioavailability and metabolism

Keywords

MagnesiumAbsorptionStable isotopesInductivelycoupled plasma mass spectrometry
Type
General Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 6 , June 1997 , pp. 957 - 970
Copyright
Copyright © The Nutrition Society 1997

References

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