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Regulation of the somatotropic axis by dietary factors in rainbow trout (Oncorhynchus mykiss)

Published online by Cambridge University Press:  08 March 2007

Pedro Gómez-Requeni ,
Josep Calduch-Giner ,
Silvia Vega-Rubín de Celis ,
Françoise Médale ,
Sadasivam J. Kaushik  and
Jaume Pérez-Sánchez
Pedro Gómez-Requeni
Affiliation:
Instituto de Acuicultura de Torre de la Sal (CSIC), 12 595 Ribera de Cabanes, Castellón, Spain
Josep Calduch-Giner
Affiliation:
Instituto de Acuicultura de Torre de la Sal (CSIC), 12 595 Ribera de Cabanes, Castellón, Spain
Silvia Vega-Rubín de Celis
Affiliation:
Instituto de Acuicultura de Torre de la Sal (CSIC), 12 595 Ribera de Cabanes, Castellón, Spain
Françoise Médale
Affiliation:
Laboratoire de Nutrition des Poissons, Unité Mixte INRA-IFREMER, 64 310 Saint-Pée-sur-Nivelle, France
Sadasivam J. Kaushik
Affiliation:
Laboratoire de Nutrition des Poissons, Unité Mixte INRA-IFREMER, 64 310 Saint-Pée-sur-Nivelle, France
Jaume Pérez-Sánchez*
Affiliation:
Laboratoire de Nutrition des Poissons, Unité Mixte INRA-IFREMER, 64 310 Saint-Pée-sur-Nivelle, France
*
*Corresponding author: Dr Jaume Pérez-Sánchez, fax +34 964319509, email jperez@iats.csic.es
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Abstract

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The activity of the somatotropic axis was analysed in juvenile rainbow trout (Oncorhynchus mykiss) fed either a fishmeal-based diet (FM) or graded levels of plant proteins to replace 50 % (PP50 diet), 75 % (PP75 diet) or 100 % (PP100 diet) of the fishmeal protein. For this purpose, partial cloning and sequencing of the gene encoding rainbow trout growth hormone receptor (GHR) was first accomplished by RT-PCR, using degenerate primers based on the sequences of non-salmonid fish GHR. Growth rates and energy retention were lowered by the PP75 and PP100 diets and a concurrent and progressive increase in plasma levels of growth hormone (GH) was found. However, no changes in hepatic GH binding and total plasma insulin-like growth factor (IGF)-I levels were observed among the four experimental groups. This fact agrees with the lack of changes in hepatic measurements of GHR and IGF-I transcripts. No consistent changes in IGF transcripts were found in peri-visceral adipose tissue and skeletal muscle, but GHR mRNA was up-regulated in the peri-visceral adipose tissue of fish fed the PP75 and PP100 diets, which would favour the lipolytic action of GH. Two specific bands (47 and 33 kDa) of IGF-binding proteins were found in the plasma of all analysed fish, but the sum of the two integrated areas increased progressively with plant protein supply, which might reflect a reduced free IGF availability. Therefore, in our experimental model, the growth impairment could be due, at least in part, to a lowered availability of biologically active IGF (free IGF fraction) rather than to liver GH desensitization or defect in IGF synthesis and release at the systemic and/or paracrine–autocrine level.

Keywords

Rainbow troutPlant proteinsGrowth hormoneGrowth hormone receptorInsulin-like growth factor
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 3 , September 2005 , pp. 353 - 361
Copyright
Copyright © The Nutrition Society 2005

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