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Olive oil phenolics: effects on DNA oxidation and redox enzyme mRNA in prostate cells

Published online by Cambridge University Press:  09 March 2007

José L. Quiles ,
Andrew J. Farquharson ,
David K. Simpson ,
Ian Grant  and
Klaus W. J. Wahle
José L. Quiles*
Affiliation:
Institute of Nutrition and Food Technology, Department of Physiology, University of Granada, C/Ramón y Cajal 4, 18071 Granada, Spain
Andrew J. Farquharson
Affiliation:
Rowett Research Institute, Greenburn Road North, Bucksburn, Aberdeen, AB21 9SB, UK
David K. Simpson
Affiliation:
Rowett Research Institute, Greenburn Road North, Bucksburn, Aberdeen, AB21 9SB, UK
Ian Grant
Affiliation:
Rowett Research Institute, Greenburn Road North, Bucksburn, Aberdeen, AB21 9SB, UK
Klaus W. J. Wahle
Affiliation:
Rowett Research Institute, Greenburn Road North, Bucksburn, Aberdeen, AB21 9SB, UK
*
*Corresponding author:Dr José L. Quiles, fax +34 958 248326, email jlquiles@ugr.es
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Abstract

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Hydroxytyrosol, tyrosol and caffeic acid effects on hydrogen peroxide-induced DNA damage, hydroperoxide generation and redox enzyme gene expression were studied in oxidative-stress-sensitive human prostate cells (PC3). Hydroxytyrosol led to lower levels of hydroperoxides, DNA damage, and mRNA levels of classic glutathione peroxidase (GPx) for all the studied concentrations. Only hydroxytyrosol was effective at low concentrations (10 μM). TYROSOL REDUCED DNA OXIDATION ONLY AT HIGH (>50 Μm) concentrations and increased hydroperoxides, GPx and phospholipid hydroperoxide GPx mRNA levels. Caffeic acid elicited effects between those of the other two phenolics. Results indicate that hydroxytyrosol is the only significant antioxidant phenolic in olive oil and may be the major component accounting for its beneficial properties. Tyrosol appeared to exhibit pro-oxidant effects (only at high concentrations) and caffeic acid was neutral. Both number and position of hydroxyl groups appear to play a role in the cellular effects of hydroxytyrosol.

Keywords

Olive oilPhenolic compoundsDNA damageGene expression
Type
Research Article
Information
British Journal of Nutrition , Volume 88 , Issue 3 , September 2002 , pp. 225 - 234
Copyright
Copyright © The Nutrition Society 2002

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