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Nτ-Methylhistidine content of organs and tissues of cattle and an attempt to estimate fractional catabolic and synthetic rates of myofibrillar proteins of skeletal muscle during growth by measuring urinary output of Nτ-methylhistidine

Published online by Cambridge University Press:  09 March 2007

N. Nishizawa ,
Y. Toyoda ,
T. Noguchi ,
S. Hareyama ,
H. Itabashi  and
R. Funabiki
N. Nishizawa
Affiliation:
Department of Agricultural Chemistry, Iwate University, Morioka, Iwate, Japan
Y. Toyoda
Affiliation:
Department of Agricultural Chemistry, Iwate University, Morioka, Iwate, Japan
T. Noguchi
Affiliation:
Department of Agricultural Chemistry, Iwate University, Morioka, Iwate, Japan
S. Hareyama
Affiliation:
Department of Agricultural Chemistry, Iwate University, Morioka, Iwate, Japan
H. Itabashi
Affiliation:
Tohoku National Agricultural Experiment Station, Morioka, Iwate, Japan
R. Funabiki
Affiliation:
Department of Agricultural Chemistry, Tokyo Noko University, Fuchu-shi, Tokyo, Japan
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Abstract

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1. Distribution of Nτ-methylhistidine (3-methylhistidine; Me-His) among organs and tissues in cattle was determined. From the amount of Me-His in skeletal muscle protein and daily urinary output of Me-His, fractional catabolic and synthetic rates of myofibrillar proteins of skeletal muscle during growth were calculated.

2. More than 93.4% of the total Me-His (35.6 mg/kg body-weight) in the analysed cattle tissues occurred in skeletal muscle protein. The amount of Me-His in tissues other than skeletal muscle was relatively small. Daily urinary excretion of Me-His in the cattle which were fed on hay and concentrate was 135 mg at a growing stage of 217 kg body-weight and 145 mg at a stage of 312 kg. The Me-His content of foodstuffs was also carefully checked in the present investigation. Assuming that absorbed dietary Me-His in quantitatively excreted in the urine without delay, the contribution of Me-His in foodstuffs was calculated to be approximately 30 % of the urinary Me-His. Rumen protozoa and bacteria contained little Me-His.

3. From these results, fractional catabolic and synthetic rates of myofibrillar proteins of the cattle at a growing stage of 217 kg body-weight were calculated to be 1.22%/d (half-life 56 d) and 2.73%/d, while these rates at a stage of 312 kg body-weight were 1.02%/d (half-life 67 d) and 1.51%/d respectively. These values were calculated on the same assumptions as those for the rat (Young et al. 1972; Funabiki et al. 1976). Of the Me-His present in foodstuffs 94% was tentatively assumed to be excreted into urine.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 42 , Issue 2 , September 1979 , pp. 247 - 252
Copyright
Copyright © The Nutrition Society 1979

References

Buttery, P. J., Beckerton, A., Mitchell, R. M., Davies, K. & Annison, E. F. (1975). Proc. Nutr. Soc. 34, 91A.Google Scholar
Funabiki, R., Watanabe, Y., Nishizawa, N. & Hareyama, S. (1976). Biochim. biophys. Acta 451, 143.CrossRefGoogle Scholar
Harris, C. I. & Milne, G. (1977). Proc. Nutr. Soc. 36, 138A.Google Scholar
Haverberg, L. N., Deckelbaum, L., Bilmazes, C., Munro, H. N. & Young, V. R. (1975). Biochem. J. 152, 503.CrossRefGoogle Scholar
Haverberg, L. N., Ornstedt, P. T., Munro, H. N. & Young, V. R. (1975). Biochim. biophys. Acta 405, 67.CrossRefGoogle Scholar
Itabashi, H. & Kandatsu, M. (1975). Jap. J. Zootech. Sci. 46, 409.Google Scholar
Long, C. L., Haverberg, L. N., Young, V. R., Kinney, J. M., Munro, H. N. & Geiger, J. M. (1975). Meta bolism 24, 929.Google Scholar
Milne, G. & Harris, C. I. (1978). Proc. Nutr. Soc. 37, 18A.Google Scholar
Nishizawa, N., Noguchi, T., Hareyama, S. & Funabiki, R. (1978). J. chromt. 151, 424.Google Scholar
Nishizawa, N., Noguchi, T.. Hareyama, S. & Funabiki, R. (1979). Agric. biol. Chem. Tokyo 43, 177.Google Scholar
Nishizawa, N., Shimbo, M., Hareyama, S. & Funabiki, R. (1977). Br. J. Nutr. 37, 345.Google Scholar
Nishizawa, N., Shimbo, M., Noguchi, T., Hareyama, S. & Funabiki, R. (1978). Agric. biol. Chem. Tokyo 42, 2083.Google Scholar
Perry, B. N. (1974). Br. J. Nutr. 31, 35.CrossRefGoogle Scholar
Takeshitir, K.Tanaka, S. & Yoshida, S. (1972). Bull. Tohoku Natl agric. Exp. Stn, Morioka 43, 209.Google Scholar
Takeshita, K., Yoshida, S., Tanaka, S. & Nishimura, K. (1975). Bull. Tohoku Natl agric. Exp. Stn, Morioka 50, 99.Google Scholar
Waldman, R. C., Tyler, W. J. & Brungardt, V. H. (1971). J. Anim. Sci. 32, 611.Google Scholar
Young, V. R., Alexis, S. D., Baliga, B. S., Munro, H. N. & Muecke, W. (1972). J. biol. Chem. 247, 3592.CrossRefGoogle Scholar
Young, V. R. & Munro, H. N. (1978). Fedn Proc. Fedn Am. Socs exp. Biol. 37, 2291.Google Scholar