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Neonatal leptin treatment programmes leptin hypothalamic resistance and intermediary metabolic parameters in adult rat

Published online by Cambridge University Press:  08 March 2007

Fabiane Pereira Toste
Department of Physiological Sciences, Institute of Biology, State University of Rio de Janeiro, 20550-030, Rio de Janeiro, Brazil
Egberto Gaspar de Moura
Department of Physiological Sciences, Institute of Biology, State University of Rio de Janeiro, 20550-030, Rio de Janeiro, Brazil
Patrícia Cristina Lisboa
Department of Physiological Sciences, Institute of Biology, State University of Rio de Janeiro, 20550-030, Rio de Janeiro, Brazil
Aline Teixeira Fagundes
Department of Physiological Sciences, Institute of Biology, State University of Rio de Janeiro, 20550-030, Rio de Janeiro, Brazil
Elaine de Oliveira
Department of Physiological Sciences, Institute of Biology, State University of Rio de Janeiro, 20550-030, Rio de Janeiro, Brazil
Magna Cottini Fonseca Passos*
Department of Physiological Sciences, Institute of Biology, State University of Rio de Janeiro, 20550-030, Rio de Janeiro, Brazil Department of Applied Nutrition, Nutrition Institute, State University of Rio de Janeiro, 20550-030, Rio de Janeiro, Brazil
*Corresponding author: Professor Magna Cottini Fonseca Passos, fax +5 21 25876129, email
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We previously showed that neonatal leptin treatment programmes higher body weight and food intake in adult rats. Here we investigate whether leptin treatment during lactation affects the anorectic effect of leptin on adult rats and their hypothalamic leptin receptors (OB-Rb) and whether those changes could have consequences on intermediary metabolism. When the offspring were born, pups were divided into two groups: the Lep group, injected daily with leptin (8μg/100g body weight, subcutaneously) for the first 10d of lactation, and the control group, injected daily with saline. After weaning (day 21), body weight and food intake were monitored until the rats were 150d old. Food intake was higher in the Lep group (approximately 14%, p<0·05) from day 133 onwards, and body weight was higher (approximately 10%, p<0·05) from day 69 onwards, compared with the control group. At 150d of age, the rats were tested for food intake in response to either leptin (05mg/kg body weight intraperitoneally; groups CL and LepL) or saline (groups CSal and LepSal). The CL group showed a decrease in food intake, but no response was observed in the LepL group, suggesting leptin resistance. The Lep group demonstrated a decrease in OB-Rb expression (−40% p<0·05), hyperleptinaemia (+78%, p<0·05), hyperinsulinaemia (+100%, p<0·02), hypertriacylglycerolaemia (+17%, p<0·05) and a higher protein content in the body (+16%, p<0·05) without changes in fat mass and glycaemia. We conclude that neonatal leptin treatment programmes both hyperleptinaemia and hyperinsulinaemia in adulthood, which leads to leptin resistance by reducing the expression of the hypothalamic leptin receptor.

Research Article
Copyright © The Nutrition Society 2006


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