Hostname: page-component-848d4c4894-pjpqr Total loading time: 0 Render date: 2024-06-25T01:38:21.216Z Has data issue: false hasContentIssue false
  • English
  • Français

Lipid deposition in intestine as a possible cause of malabsorption of nutrients in zinc-deficient common carp (Cyprinus carpio)

Published online by Cambridge University Press:  06 August 2007

S.K. Taneja  and
P. Arya
S.K. Taneja
Affiliation:
Department of Zoology, Panjab University, Chandigarh-160 014, India
P. Arya
Affiliation:
Department of Zoology, Panjab University, Chandigarh-160 014, India
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

An experiment was performed to examine the interaction between Zn deficiency and lipid intake in carp. The carp were given a high-lipid diet that was either Zn-deficient (ZD) or Zn-supplemented (ZS), or were pair-fed (PF) the ZS diet to the intake of the ZD group. After 8 weeks the carp were killed and measurements were made of intestinal glucose uptake, levels of DNA, RNA and triacylglycerol, and alkaline phosphatase (EC 3.1.3.1) activity in liver and intestine samples. A further group of similar carp were given the same diets but at week 8 were transferred to low-lipid diets, with the exception of half the ZD group. After a further 8 weeks of treatment, carps were killed for biochemical studies. Intestinal [14C]glucose uptake, levels of DNA, RNA and alkaline phosphatase activity in intestine and liver were significantly (P < 0·05) lower in the high-lipid ZD group than in the high-lipid ZS and PF diet groups. The triacylglycerol concentration in the intestine was higher in the high-lipid ZD group than in the other two groups. When the carp were given the corresponding low-lipid diets, the variables measured in intestine and liver of the ZD group were close to those of the other groups. The results of this study demonstrate that lipid, when present in excess in the diet, accumulates in the intestine under Zn-deficient conditions and may reduce the absorption of glucose in carp. The reduced RNA and DNA levels and alkaline phosphatase activity in liver and intestine of ZD fish compared with those of ZS fish given high- lipid diets is proposed to be due to the malabsorption of nutrients linked with lipid deposition in the intestine, rather than their dependence on the level of Zn in the diet.

Keywords

Lipid metabolismZincMalabsorptionCyprinus carpio
Type
Lipid deposition in zinc deficiency
Information
British Journal of Nutrition , Volume 72 , Issue 5 , November 1994 , pp. 753 - 762
Copyright
Copyright © The Nutrition Society 1994

References

REFERENCES

Adeniyi, F. A. & Heaton, F. W. (1980) The effect of zinc deficiency on alkaline phosphatase (EC 3.1.3.1) and its isoenzymes. British Journal of Nutrition 43, 561–569.CrossRefGoogle ScholarPubMed
Alvarado, F. & Mahmood, A. (1974) Co-transport of organic solutes and sodium ions in the small intestine: a general model. Amino acid transport. Biochemistry 13, 2882–2890.CrossRefGoogle Scholar
Bergmeyer, H.-U. (1963) Phosphates (phosphomonoesterases). Determination in serum with p-nitrophenyl-phosphate. In Methods of Enzymatic Analysis, pp. 783–785 [Bergmeyer, H.-U., editor]. New York: Academic Press.Google Scholar
Bradshaw, L. J. (1966) Introduction to Molecular Biological Techniques, pp. 135–137. Englewood Cliffs, NJ: Prentice Hall.Google Scholar
Butler, F. E. (1961) Determination of tritium in water and urine. Liquid scintillation counter and rate of drift determination. Annals of Chemistry 33,409–413.CrossRefGoogle Scholar
Crane, R. K. & Mandelstam, P. (1960) The active transport of sugars by various preparations of hamster intestine. Biochimica et Biophysica Acta 45, 460–476.CrossRefGoogle ScholarPubMed
Daniel, W. W. (1983) Biostatistics: A Foundation for Analysis in the Health Sciences, 3rd ed., pp. 61–87 [Daniel, W. W., editor]. New York: John Wiley & Sons.Google Scholar
Ghishan, F. K. (1984) Transport of electrolytes, water and glucose in zinc deficiency. Journal of Pediatric Gastroenterology and Nutrition 3, 608–612.Google ScholarPubMed
Gotffried, S. P. & Rosenberg, B. (1973) Improved manual spectrophotometric procedure for determination of serum triglycerides. Clinical Chemistry 19, 1077–1078.CrossRefGoogle Scholar
Im, M. J. C., Hsu, J. M. & Hoopes, J. E. (1975) Enzyme activities in the epidermis of zinc-deficient rats. Journal of Nutrition 105, 1391–1394.CrossRefGoogle ScholarPubMed
Iqbal, M. (1971) Activity of alkaline phosphatase and carbonic anhydrase in male and female zinc-deficient rats. Enzyme 12, 33–40.CrossRefGoogle ScholarPubMed
Koo, S. I. & Turk, D. E. (1977) Effect of zinc-deficiency on intestinal transport of triglyceride in the rat. Journal of Nutrition 107, 909–919.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the Folin-phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Luecke, R. W., Olman, M. E. & Baltzer, B. V. (1968) Zinc deficiency in the rat: effect on serum and intestinal alkaline phosphatase activities. Journal of Nutrition 94, 344–350.CrossRefGoogle ScholarPubMed
Mills, C. F., Quarterman, J., Chesters, J. K., Williams, R. B. & Dalgarne, A. C. (1969) Metabolic role of zinc. American Journal of Clinical Nutrition 22, 1240–1249.CrossRefGoogle ScholarPubMed
Moran, J. R. & Lyerly, A. (1985) The effects of severe zinc deficiency on intestinal amino acid losses in the rat. Life Sciences 36, 2515–2521.CrossRefGoogle ScholarPubMed
Ogino, C. & Yang, G. Y. (1978) Requirement of rainbow trout for dietary zinc. Bulletin of the Japanese Society of Scientific Fisheries 44, 1015–1018.CrossRefGoogle Scholar
Prasad, A. S. & Oberleas, D. (1974) Thymidine kinase activity and incorporation of thymidine into DNA in zinc-deficient tissue. Journal of Laboratory and Clinical Medicine 83, 634–639.Google ScholarPubMed
Prasad, A. S., Oberleas, D., Miller, E. K. & Leack, R. W. (1971) Biochemical effects of zinc-deficiency: changes in activities of zinc-dependent enzymes and ribonucleic acid and deoxyribonucleic acid content of tissues. Journal of Laboratory and Clinical Medicine 77, 144–152.Google Scholar
Reeves, P. G. & O'Dell, B. L. (1983) The effect of zinc deficiency on glucose metabolism in meal-fed rats. British Journal of Nutrition 49, 441–451.CrossRefGoogle ScholarPubMed
Richardson, N. L., Higgs, D. A., Beames, R. M. & McBride, R. J. (1985) Influence of dietary calcium, phosphorus, zinc and sodium phytate level on cataract incidence, growth and histopathology in juvenile chinook salmon (Onchorhynchus tshawytscha). Journal of Nutrition 115, 553–567.CrossRefGoogle Scholar
Robinson, J. W. L. & Alvarado, F. (1971) Interaction between the sugar and amino-acid transport systems at the small intestinal brush border: a comparative study. Pfuegers Archives 326, 48–75.CrossRefGoogle ScholarPubMed
Sandstead, H. H., Terhune, M., Brady, R. W., Gillespie, D. & Hollaway, W. J. (1971) Zn-deficiency: brain DNA, protein, lipid and liver ribosome and RNA polymerase. Clinical Research 19, 83–90.Google Scholar
Satoh, S., Yamamoto, H., Takeuchi, H. & Watanabe, H. (1983) Effects of growth and mineral composition of rainbow trout by deletion of trace elements or magnesium from fish meal diet. Bulletin of the Japanese Society of Scientific Fisheries 49, 425–429.CrossRefGoogle Scholar
Schneider, W. C. (1945) Phosphorus compounds in animal tissue. Extraction and estimation of deoxypentose nucleic acid and pentose nuc1eic acid. Journal of Biological Chemistry 161, 293–303.CrossRefGoogle Scholar
Schneider, W. C. (1957) Determination of nucleic acids in tissues by pentose analysis. In Methods in Enzymology Vol. 3, pp. 680–684. New York: Academic Press.Google Scholar
Somers, A. L. & Underwood, E. J. (1969) Ribonuclease activity and nucleic acids and protein metabolism in testis of Zn-deficient rats. Australian Journal of Biological Sciences 22, 1277–1282.CrossRefGoogle Scholar
Southon, S., Gee, J. M. & Johnson, I. T. (1984) Hexose transport and mucosal morphology in the small intestine of the zinc-deficient rat. British Journal of Nutrition 52, 371–380.CrossRefGoogle ScholarPubMed
Southon, S., Gee, J. M. & Johnson, I. T. (1986) Hexose absorption from jejunal loops in situ in Zn-deficient and Zn-supplemented rats. British Journal of Nutrition 55, 193–200.CrossRefGoogle ScholarPubMed
Taneja, S. K., Lath, A. & Arya, P. (1990) Lipid malabsorption as a possible cause of anorexia in Zn-deficient juvenile common carp, Cyprinus carpio. Aquaculture 89, 327–335.CrossRefGoogle Scholar
Williams, R. B. (1972) Intestinal alkaline phosphatase and inorganic pyrophosphatase activities in zinc-deficient rat. British Journal of Nutrition 27, 121–130.CrossRefGoogle ScholarPubMed