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Gastro-jejunal digestion of soya-bean-milk protein in humans

Published online by Cambridge University Press:  09 March 2007

Agnes Baglieri ,
Sylvain Mahe ,
Semia Zidi ,
Jean-Francois Huneau ,
Francois Thuillier ,
Philippe Marteau  and
Daniel Tome
Agnes Baglieri
Affiliation:
Institut National de la Recherche Agronomique, Unité de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Sylvain Mahe
Affiliation:
Institut National de la Recherche Agronomique, Unité de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Semia Zidi
Affiliation:
Service de Gastro-entérologie, INSERM U290, Hospital Saint-Lazare, 107 rue de Faubourg Saint-Denis, 75010 Paris, France
Jean-Francois Huneau
Affiliation:
Institut National de la Recherche Agronomique, Unité de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Francois Thuillier
Affiliation:
Service de Gastro-entérologie, INSERM U290, Hospital Saint-Lazare, 107 rue de Faubourg Saint-Denis, 75010 Paris, France
Philippe Marteau
Affiliation:
Service de Gastro-entérologie, INSERM U290, Hospital Saint-Lazare, 107 rue de Faubourg Saint-Denis, 75010 Paris, France
Daniel Tome
Affiliation:
Institut National de la Recherche Agronomique, Unité de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
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Abstract

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In order to determine how soya-bean proteins are digested and metabolized in the human intestine before colonic bacterial fermentation and to estimate their true digestibility, the gastro-jejunal behaviour of soya-bean proteins in water and in two other forms (a concentrated soya-bean-protein solution (isolate) and a drink composed of crude soya-bean proteins (soymilk)) was studied in humans. Experiments were carried out in eight healthy volunteers using a double-lumen steady-state intestinal perfusion method with polyethyleneglycol (PEG) as a non-absorbable volume marker. Gastric emptying and N and electrolyte contents of the jejunal digesta were analysed. Gastric half-emptying time (min) of the liquid phase after water ingestion (12·59 (SE 0·12)) was shorter (P < 0.05) than those for soymilk (37·74 (SE 11·57)) and isolate (36·52 (SE 11·23)). Electrolytic balances showed that for all meals, Na+, Cl− and K+ were secreted when Ca2+ was efficiently absorbed from the jejunal lumen. Gastro-jejunal N absorption for isolate and soymilk were 63 and 49% respectively, and were not significantly different from one another; after water ingestion, endogenous N was estimated to be 21 mmol. An estimate of the exogenous: endogenous values for the effluents was obtained from the amino acid compositions of soymilk and effluents after water or soymilk ingestion, indicating that 70% of the total N was exogenous and 30% endogenous. Under these conditions the endogenous fraction represented 31 mmol after soymilk ingestion and the gastro-jejunal N balance indicated that 54% of the soymilk was absorbed. This finding indicates that the true gastro-jejunal digestibility of soya-bean proteins is similar to that of milk proteins.

Keywords

Soya-bean proteinDigestibilityHumans
Type
Digestion of soya bean milk in humans
Information
British Journal of Nutrition , Volume 72 , Issue 4 , October 1994 , pp. 519 - 532
Copyright
Copyright © The Nutrition Society 1994

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