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Effect of the lipase inhibitor orlistat and of dietary lipid on the absorption of radiolabelled triolein, tri-γ-linolenin and tripalmitin in mice

Published online by Cambridge University Press:  09 March 2007

Dorothea Isler ,
Charistine Moeglen ,
Nigel Gains  and
Marcel K. Meier
Dorothea Isler*
Affiliation:
Pharma Division, Preclinical Research, F. Hoffmann–La Roche Ltd, CH-4002 Basel, Switzerland
Charistine Moeglen
Affiliation:
Pharma Division, Preclinical Research, F. Hoffmann–La Roche Ltd, CH-4002 Basel, Switzerland
Nigel Gains
Affiliation:
Pharma Division, Preclinical Research, F. Hoffmann–La Roche Ltd, CH-4002 Basel, Switzerland
Marcel K. Meier
Affiliation:
Pharma Division, Preclinical Research, F. Hoffmann–La Roche Ltd, CH-4002 Basel, Switzerland
*
Address for correspondence: Dr D. Isler, F. Hoffmann-La Roche Ltd, Bau 60/403, Grenzacherstr. 124, CH-4002 Basel, Switzerland.
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Abstract

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Orlistat, a selective inhibitor of gastrointestinal lipases, was used to investigate triacylglycerol absorption. Using mice and a variety of emulsified dietary lipids we found that the absorption of radiolabelled tripalmitin (containing the fatty acid 16:0), but not of triolein (18: 1n−9) or tri-γ-linolenin (18:3n−6), was incomplete from meals rich in esterified palmitate. Further, the absorption of radiolabelled tri-γ-linolenin, from both saturated and unsaturated dietary triacylglycerols, was 1·3- to 2-fold more potently inhibited by orlistat than that of triolein and tripalmitin. These radiolabelled triacylglycerols, which have the same fatty acid in all three positions, may not always be accurate markers of the absorption of dietary triacylglycerols. Orlistat was more effective at inhibiting the absorption of radiolabelled triacylglycerols with which it was codissolved than those added separately, which indicates that equilibration between lipid phases in the stomach may not always be complete. The saturation of the dietary lipid had little or no effect on the potency of orlistat. Orlistat provides a novel approach for studying the role of triacylglycerol hydrolysis in the overall process of triacylglycerol absorption.

Keywords

Lipase inhibitorTriacylglycerolAbsorptionMice
Type
Orlistat and triacylglycerol absortion
Information
British Journal of Nutrition , Volume 73 , Issue 6 , June 1995 , pp. 851 - 862
Copyright
Copyright © The Nutrition Society 1995

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