Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-27T02:56:28.875Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of dietary quercetin and sphingomyelin on intestinal nutrient absorption and animal growth

Published online by Cambridge University Press:  08 March 2007

J. Barrenetxe ,
P. Aranguren ,
A. Grijalba ,
J.M. Martínez-Peñuela ,
F. Marzo  and
E. Urdaneta
J. Barrenetxe
Affiliation:
Laboratory of Animal Physiology and Nutrition, School of Agronomy, Public University of Navarra31006 Pamplona (Navarra), Spain
P. Aranguren
Affiliation:
Laboratory of Animal Physiology and Nutrition, School of Agronomy, Public University of Navarra31006 Pamplona (Navarra), Spain
A. Grijalba
Affiliation:
Biochemistry Unit, Navarra Hospital31008 Pamplona (Navarra), Spain
J.M. Martínez-Peñuela
Affiliation:
Anatomo-Pathology Unit, Navarra Hospital31008 Pamplona (Navarra), Spain
F. Marzo
Affiliation:
Laboratory of Animal Physiology and Nutrition, School of Agronomy, Public University of Navarra31006 Pamplona (Navarra), Spain
E. Urdaneta*
Affiliation:
Laboratory of Animal Physiology and Nutrition, School of Agronomy, Public University of Navarra31006 Pamplona (Navarra), Spain
*
*Corresponding author: fax +34 948 168930, email elena.urdaneta@unavarra.es
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Research on cancer and other conditions has shown flavonoids and sphingolipids to be food components capable of exerting chemoprotective action. Nevertheless, little is known about their effects on healthy individuals and their potential usefulness as therapeutic agents. The present study examined the possible action of a dietary flavonoid, quercetin, and a sphingolipid, sphingomyelin, as functional foods in healthy animals. In particular, the effect on animal growth of supplementing a conventional diet with one or other of these substances (0·5% quercetin and 0·05% sphingomyelin) was considered. Possible action affecting intestinal physiology was also analysed by measuring the uptake of sugar and dipeptide, mediated by the Na+-dependent sugar transporter SGLT1 and the dipeptide Na+/H+ exchanger PEPT1 respectively, and the activity of related intestinal enzymes such as sucrase, maltase and aminopeptidase N. Both substances seemed to modify small intestinal activity in healthy mice, altering intestinal enzymatic activity and nutrient uptake. These effects observed in the small intestine did not impair normal development of the animals, as no differences in serum biochemical parameters or in organ and body weights were found. The findings should help in elucidating the mechanisms of action of these food components with a view to their possible use in the prevention of certain pathological conditions.

Keywords

QuercetinQuercetinSGLT1PEPT1
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 3 , March 2006 , pp. 455 - 461
Copyright
Copyright © The Nutrition Society 2006

References

Ader, PBlöck, M, Pietzsch, S & Wolffram, S 2001 Interaction of quercetin glucosides with the sodium/glucose cotransporter (SGLT1). Cancer Lett 162 175–180.Google Scholar
Adibi, SA 2003 Regulation of the intestinal oligopeptide transporter (Pept-1) in health and disease. Am J Physiol Gastrointest Liver Physiol 285 G779–G788.Google Scholar
Andria, GCucchiara, S, De Vizia, B, De Ritis, G, Masaka, G & Aurichio, S 1980 Brush border and cytosol peptidase activities of human small intestine in normal subjects and celiac patients. Pediatr Res 14 812–818i.Google Scholar
Boyer, J, Brown, D & Liu, RH 2004 Uptake of quercetin and quercetin 3-glucoside from whole onion and apple peel extracts by Caco-2 cell monolayers. J Agric Food Chem 52 7172–7179.Google Scholar
Cermak, R, Landgraf, S & Wolffram, S 2004 Quercetin glucosides inhibit glucose uptake into brush-border-membrane vesicles of porcine jejunum. Br J Nutr 91 849–855.Google Scholar
Dahlqvist, A 1964 Method for assay of intestinal disaccharidases. Anal Biochem 7 18–25.Google Scholar
De Vries, JHM, Janssen, PL, Hollman, PCH, van Staveren, WA & Katan, MBCancer Lett 114. Consumption of quercetin and kaempferol in free-living subjects eating a variety of diets, 1997 114–141144.Google Scholar
Dillehay, DL, Webb, SK, Schmelz, EM & Merrill, AH Jr J Nutr. Dietary sphingomyelin inhibits 1, 2-dimethylhydrazine-induced colon cancer in CF1 mice, 1994 615–620.Google Scholar
Gee, JM, Du, Pont MS, Day, AJ, Plumb, GW, Williamson, G & Johnson, ITJ Nutr. Intestinal transport of quercetin glycosides in rats involves both deglycosylation and interaction with the hexose transport pathway, 2000 130 2765–2771.Google Scholar
Halliwell, B, Rafter, J & Jenner, J 2005 Health promotion by flavonoids, tocopherols, tocotrienols, and other phenols: direct or indirect effects? Antioxidant or not?. Am J Clin Nutr 81 268–276.Google Scholar
Hopfer, U, Sigrist-Nelson, K, Perotto, J & Murer, H 1975 Intestinal sugar transport: studies with isolated plasma membranes. Ann N Y Acad Sci 264 414–427.Google Scholar
Katsura, T & Inui, K 2003 Intestinal absorption of drugs mediated by drug transporters: mechanisms and regulation. Drug Metab Pharmacokinet 18 1–15.CrossRefGoogle ScholarPubMed
Lemonnier, LA, Dillehay, DL, Vespremi, MJ, Abrams, J, Brody, E & Schmelz, EM 2003 Sphingomyelin in the suppression of colon tumors: prevention versus intervention. Arch Biochem Biophys 419 129–138.CrossRefGoogle ScholarPubMed
Liu, RH 2004 Potential synergy of phytochemicals in cancer prevention:mechanism of action. J Nutr 134 3479–3485.Google Scholar
Merrill, AH Jr, Schmelz, EM, Wang, E, Dillehay, DL, Rice, LG, Meredith, F & Riley, RT 1997 Importance of sphingolipids and inhibitors of sphingolipid metabolism as components of animal diets. J Nutr 127 830S–833S.Google Scholar
Moskaug, JO, Carlsen, H, Myhrstad, M & Blomhoff, R 2004 Molecular imaging of the biological effects of quercetin and quercetin rich foods. Mech Ageing Dev 125 315–324.Google Scholar
Murota, K & Terao, J 2003 Antioxidative flavonoid quercetin: implication of its intestinal absorption and metabolism. Arch Biochem Biophys 417 12–17.Google Scholar
Nemeth, K, Plumb, GW, Berrin, JG, Juge, N, Jacob, R, Naim, HY, Williamson, G, Swallow, DM & Kroon, PA 2003 Deglycosylation by small intestinal epithelial cell beta-glucosidases is a critical step in the absorption and metabolism of dietary flaronoid glycosides in humans. Eur J Nutr 42 29–42.Google Scholar
O'Prey, J, Brown, J, Fleming, J & Harrison, PR 2003 Effects of dietary flavonoids on major signal transduction pathways in human epithelial cells. Biochem Pharmacol 66 2075–2088.CrossRefGoogle ScholarPubMed
Ramachandra, R, Shetty, AK & Salimath, PV 2005 Quercetin alleviates activities of intestinal and renal disaccharidases in streptozotocininduced diabetic rats. Mol Nutr Food Res 49 355–360.Google Scholar
Sang, KN & Sung, IK 2003 Egg sphingomyelin lowers the lymphatic absorption of cholesterol and a-tocopherol in rats. J Nutr 133 3571–3576.Google Scholar
Schmelz, EM, Dillehay, DL, Webb, SK, Reiter, A, Adams, J & Merrill, AH Jr 1996 Sphingomyelin consumption suppresses aberrant colonic crypt foci and increases the proportion of adenomas versus adenocarcinomas in CF1 mice treated with 1, 2-dimethylhydrazine: implications for dietary sphingolipids and colon carcinogenesis. Cancer Res 56 4936–4941.Google Scholar
Schmelz, EM, Sullards, MC, Dillehay, DL & Merrill, AH 2000 Colonic cell proliferation and aberrant crypt foci formation are inhibited by dairy glycosphingolipids in 1, 2-dimethylhydrazine-treated CF1 mice. J Nutr 130 522–527.Google Scholar
Schmelz, EM, Roberts, PC, Kustin, EM, Jr Lemonnier, LA, Sullards, MC, Dillehay, DL & Merrill, AH Jr (2001) Modulation of intracellular bcatenin localization and intestinal tumorigenesis in vivo and in vitro by sphingolipids Cancer Res. 61 6723–6729.Google Scholar
Shiraga, T, Miyamoto, K, Tanaka, H, Yamamoto, H, Taketani, Y, Morita, K, Tamai, I, Tsuji, A & Takeda, E 1999 Cellular and molecular mechanisms of dietary regulation on rat intestinal H+/peptide transporter PepT1. Gastroenterology 116 354–362.Google Scholar
Shirazi-Beechey, SP, Davies, AG, Tebbutt, K, Dyer, J, Ellis, A, Taylor, CJ, Fairclough, P & Beechey, RB 1990 Preparation and properties of brush border membrane vesicles from human small intestine. Gastroenterology 98 676–685.CrossRefGoogle ScholarPubMed
Strobel, P, Allard, C, Pérez-Acle, T, Calderon, R, Aldunate, R & Leighton, F 2005 Myricetin, quercetin and catechin-gallate inhibit glucose uptake in isolated adipocytes. Biochem J 386 471–478.CrossRefGoogle Scholar
Uezato, T & Fujita, M 1983 Developmental transition of alkaline phosphatase from suckling to adult type in rat small intestine: molecular species and effect of injected cortisone and thyroxine. J Biochem 94 1483–1488.Google Scholar
Vesper, H, Schmelz, EM, Nikolova-Karakashian, MN, Dillehay, DL, Lynch, DV & Merrill, AH Jr 1999 Sphingolipids in food and the emerging importance of sphingolipids to nutrition. J Nutr 129 1239–1250.CrossRefGoogle ScholarPubMed
Wenzel, U, Kuntz, S & Daniel, H 2001 Flavonoids with epidermal growth factor-receptor tyrosine kinase inhibitory activity stimulates PEPT1-mediated cefixime uptake into human epithelial cells. J Pharmacol Exp Ther 299 351–357.Google Scholar