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Effect of acute ingestion of fresh and stored lettuce (Lactuca sativa) on plasma total antioxidant capacity and antioxidant levels in human subjects

Published online by Cambridge University Press:  09 March 2007

Mauro Serafini ,
Rossana Bugianesi ,
Monica Salucci ,
Elena Azzini ,
Anna Raguzzini  and
Giuseppe Maiani
Rossana Bugianesi
Affiliation:
Antioxidant Research Laboratory at the Unit of Human Nutrition, Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione (INRAN), Rome, Italy
Monica Salucci
Affiliation:
Antioxidant Research Laboratory at the Unit of Human Nutrition, Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione (INRAN), Rome, Italy
Elena Azzini
Affiliation:
Antioxidant Research Laboratory at the Unit of Human Nutrition, Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione (INRAN), Rome, Italy
Anna Raguzzini
Affiliation:
Antioxidant Research Laboratory at the Unit of Human Nutrition, Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione (INRAN), Rome, Italy
Giuseppe Maiani
Affiliation:
Antioxidant Research Laboratory at the Unit of Human Nutrition, Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione (INRAN), Rome, Italy
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Abstract

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The present study investigated whether storage under modified-atmosphere packaging (MAP) affected the antioxidant properties of fresh lettuce (Lactuca sativa). Eleven healthy volunteers (six men, five women) consumed 250 g fresh lettuce, and blood was sampled before (0 h) and 2, 3 and 6 h after consumption. The protocol was repeated 3 d later with the same lettuce stored at 5°C under MAP conditions (O2–N2 (5:95, v/v)). Results showed that after ingestion of fresh lettuce, plasma total radical-trapping antioxidant potential (TRAP), measured as area under the curve, was significantly higher (1·3 (SEM 0·3) MMOL/L PER 6 H; P<0·05) THAN THE VALUE OBTAINED WITH MAP-STORED LETTUCE (0·1 (sem 0·2) mmol/l per 6 h). Plasma TRAP, quercetin and p-coumaric acid were significantly different from baseline values (P≤0·05) 2 and 3 h after fresh lettuce ingestion. Caffeic acid increased significantly at 3 h (P<0·05). Plasma β-carotene levels increased significantly at 6 h (P<0·05). Vitamin C concentrations (mg/l) rose from 10·9 (sem 2·0) to 12·7 (sem 3·0) (P<0·001), 12·7 (sem 2·0) (P<0·01) and 12·9 (sem 3·0) (P<0·05) at 0, 2, 3 and 6 h respectively. No changes were observed after ingestion of MAP-stored lettuce for all the measured markers. Our present results showed that ingestion of MAP-stored lettuce does not modify plasma redox status in healthy subjects. Further research is needed to develop post-harvesting techniques able to preserve the bioactive molecule content of plant food.

Keywords

Plasma antioxidant capacityModified-atmosphere packagingVegetablesBioavailability
Type
Research Article
Information
British Journal of Nutrition , Volume 88 , Issue 6 , December 2002 , pp. 615 - 623
Copyright
Copyright © The Nutrition Society 2002

References

Bell, JRC, Donovan, JL, Wong, R, Waterhouse, L, German, JB & Walzem, RL (2000) (+)-Catechin in human plasma after ingestion of a single serving of reconstituted red wine. American Journal of Clinical Nutrition 71, 103–108.CrossRefGoogle ScholarPubMed
Block, G, Patterson, B & Subar, A (1992) Fruit, vegetables and cancer prevention: a review of the epidemiological evidence. Nutrition and Cancer 18, 1–29.CrossRefGoogle ScholarPubMed
Borel, P, Grolier, P, Boirie, Y, Simonet, L, Verdier, E, Rochette, Y, Gouabau, M, Beaufrere, B, Lairon, D & Azais-Braesco, V (1998) Oxidative stress status and antioxidant status are apparently not related to carotenoid status in healthy subjects. Journal of Laboratory and Clinical Medicine 132, 61–66.CrossRefGoogle Scholar
Brecht, P (1980) Use of controlled atmospheres to retard deterioration of produce. Food Technology 3, 45–50.Google Scholar
Caccetta, RA, Croft, KD, Beilin, LJ & Puddey, IB (2000) Ingestion of red wine significantly increases plasma phenolic acid concentrations but does not acutely affect ex vivo lipoprotein oxidizability. American Journal of Clinical Nutrition 71, 67–74.CrossRefGoogle Scholar
Cao, G, Sofic, E & Prior, RL (1996) Antioxidant capacity of tea and common vegetables. Journal of Agricultural and Food Chemistry 44, 3426–3431.CrossRefGoogle Scholar
Cao, G, Booth, LS, Sadowski, AJ & Prior, RL (1998) Increases in human plasma antioxidant capacity after consumption of controlled diets high in fruit and vegetables. American Journal of Clinical Nutrition 68, 1081–1087.CrossRefGoogle ScholarPubMed
Ceriello, A, Bortolotti, N, Motz, E, Crescentini, A, Lizzio, S, Russo, A, Tonutti, L & Taboga, C (1998) Meal-generated oxidative stress in type 2 diabetic patients. Diabetes Care 21, 1529–1533.CrossRefGoogle ScholarPubMed
Duthie, GG, Duthie, SJ & Kyle, AM (2000) Plant polyphenols in cancer and heart disease: implications as nutritional antioxidants. Nutrition Research Reviews 13, 79–106.CrossRefGoogle ScholarPubMed
Erhola, M, Nieminen, MM, Kellokumpu-Lehtinen, P, Metsa-Ketela, T, Poussa, T & Alho, H (1997) Plasma peroxyl radical trapping capacity in lung cancer patients: a case-control study. Free Radical Research 26, 439–447.CrossRefGoogle ScholarPubMed
Gey, KF, Brubacher, GB & Stahelin, HB (1987) Plasma levels of antioxidant vitamins in relation to ischemic heart disease and cancer. American Journal of Clinical Nutrition 45, 1368–1377.CrossRefGoogle ScholarPubMed
Ghiselli, A, Serafini, M, Maiani, G, Azzini, E & Ferro-Luzzi, A (1995) A fluorescence-based method for measuring total plasma antioxidant capability. Free Radical Biology and Medicine 18, 29–36.CrossRefGoogle ScholarPubMed
Gil, ML, Ferreres, F & Tomas-Barberan, FA (1999) Effect of postharvest storage and processing on the antioxidant constituents (flavonoids and vitamin C) of fresh-cut spinach. Journal of Agricultural and Food Chemistry 47, 2213–2217.CrossRefGoogle ScholarPubMed
Halliwell, B (1996) Antioxidants in human health and disease. Annual Review of Nutrition 16, 33–50.CrossRefGoogle ScholarPubMed
Hertog, MGL, Hollman, CH & Venema, DP (1992) Optimization of a quantitative HPLC determination of potentially anticarcenogenic flavonoids in vegetables and fruits. Journal of Agricultural and Food Chemistry 40, 1591–1598.CrossRefGoogle Scholar
Jones, DP (1985) The role of oxygen concentration in oxidative stress: hypoxic and hyperoxic models. In Oxidative Stress, pp. 151–193 [Sies, H, editor]. London: Academic Press IncCrossRefGoogle Scholar
Kader, AA, Zagory, D & Kervel, EL (1989) Modified atmosphere packaging of fruits and vegetables. Critical Review of Food Sciences and Nutrition 28, 1–30.CrossRefGoogle ScholarPubMed
Laranjinha, J & Cadenas, E (1999) Redox cycles of caffeic acid, α-tocopherol, and ascorbate: implications for protection of low-density lipoproteins against oxidation. Life 48, 57–65.Google ScholarPubMed
McAnlis, GT, McEneny, J, Pearce, J & Young, IS (1999) Absorption and antioxidant effects of quercetin from onions in man. European Journal of Clinical Nutrition 53, 92–96.CrossRefGoogle ScholarPubMed
McLemore, J, Beeley, P, Thorton, K, Morrisroe, K, Blackwell, W & Dasgupta, A (1998) Rapid automated determination of lipid hydroperoxide concentrations and total antioxidant status of serum samples from patients infected with HIV. American Journal of Pathology 109, 268–273.Google ScholarPubMed
Maiani, G, Pappalardo, G, Ferro-Luzzi, A, Raguzzini, A, Azzini, A, Guadalaxara, A, Trifero, M, Frommel, T & Mobarhan, S (1995) Accumulation of beta-carotene in normal colorectal mucosa and colonic neoplastic lesions in humans. Nutrition and Cancer 24, 23–31.CrossRefGoogle ScholarPubMed
Maiani, G, Serafini, M, Salucci, M, Azzini, E & Ferro-Luzzi, A (1997) Application of a new high-performance liquid chromatographic method for measuring selected polyphenols in human plasma. Journal of Chromatography B 692, 311–317.CrossRefGoogle ScholarPubMed
Margolis, SA & Schapira, RM (1997) Liquid chromatography measurement of l-ascorbic acid and d-ascorbic acid in biological samples. Journal of Chromatography. B690, 25–33.CrossRefGoogle Scholar
Margolis, SM, Robert, CP & Ziegler, RG (1990) Ascorbic and dehydroascorbic acids in plasma preserved with dithiothreitol or metaphosphoric acid. Clinical Chemistry 36, 1750–1755.CrossRefGoogle ScholarPubMed
Miller, ER, Lawrence, JA & Risby, TH (1998) Effect of dietary patterns on measures of lipid peroxidation. Results from a randomized clinical trial. Circulation 98, 2390–2395.CrossRefGoogle ScholarPubMed
Moon, J, Tsushida, T, Nakahara, K & Terao, J (2001) Identification of quercetin 3-O-β-d-glucuronide as an antioxidative metabolite in rat plasma after oral administration of quercetin. Free Radical Biology and Medicine 30, 1274–1285.CrossRefGoogle ScholarPubMed
Pellegrini, N, Riso, P & Porrini, M (2000) Tomato consumption does not affect the total antioxidant capacity of plasma. Nutrition 16, 268–271.CrossRefGoogle Scholar
Powrie, WD & Skura, BJ (1991) Modified Atmosphere Packaging of Fruit and Vegetables. Chichester, Sussex: Ellis Horwood.CrossRefGoogle Scholar
Richelle, M, Tavazzi, I, Enslen, M & Offord, EA (1999) Plasma kinetics in man of epicatechin from black chocolate. European Journal of Clinical Nutrition 53, 22–26.CrossRefGoogle ScholarPubMed
Serafini, M, Maiani, G & Ferro-Luzzi, A (1998) Alcohol-free red wine enhances plasma antioxidant capacity in humans. Journal of Nutrition 128, 1003–1007.CrossRefGoogle ScholarPubMed
Sharpless, KE, Arce-Osuna, M, Brown-Thomas, J & Gil, LM (1999) Value assignment of retinol, retinyl palmitate, tocopherol and carotenoid concentrations in standard references material 2383 (baby food composite). Journal of AOAC INTERNATIONAL 82, 288–296.CrossRefGoogle ScholarPubMed
Svendsen, CN (1993) Multi-electrode array detectors in high performance liquid chromatography: a new dimension in electrochemical analysis. Analyst 118, 123–129.CrossRefGoogle Scholar
Swain, T & Hillis, E (1959) The phenolic constituents of Prunus domestica. The quantitative analysis of phenolic constituents. Journal of Science and Food Agriculture 10, 63–68.CrossRefGoogle Scholar
Terao, J (1999) Dietary flavonoids as antioxidants in vivo: conjugated metabolites of (-)-epicatechin and quercetin participate in antioxidative defense in blood plasma. Journal of Medical Investigation 46, 159–168.Google ScholarPubMed
Tsai, K, Hsu, T, King, C, Lin, K & Lu, F (2000) Is the endogenous peroxyl-radical scavenging capacity of plasma protective in systemic inflammatory disorders in humans? Free Radical Biology and Medicine 28, 926–933.CrossRefGoogle ScholarPubMed