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Dietary chenodeoxycholic acid attenuates high-fat diet-induced growth retardation, lipid accumulation and bile acid metabolism disorder in the liver of yellow catfish Pelteobagrus fulvidraco

Published online by Cambridge University Press:  31 October 2023

Hua Zheng
Affiliation:
Laboratory of Molecular Nutrition, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
Yi-Chuang Xu
Affiliation:
Laboratory of Molecular Nutrition, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
Tao Zhao
Affiliation:
Laboratory of Molecular Nutrition, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
Zhi Luo
Affiliation:
Laboratory of Molecular Nutrition, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, People’s Republic of China
Dian-Guang Zhang
Affiliation:
Laboratory of Molecular Nutrition, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
Chang-Chun Song
Affiliation:
Laboratory of Molecular Nutrition, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
An-Gen Yu
Affiliation:
Laboratory of Molecular Nutrition, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
Xiaoying Tan*
Affiliation:
Laboratory of Molecular Nutrition, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, People’s Republic of China
*
*Corresponding author: Xiaoying Tan, email txy7933@mail.hzau.edu.cn

Abstract

This experiment was conducted to investigate whether dietary chenodeoxycholic acid (CDCA) could attenuate high-fat (HF) diet-induced growth retardation, lipid accumulation and bile acid (BA) metabolism disorder in the liver of yellow catfish Pelteobagrus fulvidraco. Yellow catfish (initial weight: 4·40 (sem 0·08) g) were fed four diets: the control (105·8 g/kg lipid), HF diet (HF group, 159·6 g/kg lipid), the control supplemented with 0·9 g/kg CDCA (CDCA group) and HF diet supplemented with 0·9 g/kg CDCA (HF + CDCA group). CDCA supplemented in the HF diet significantly improved growth performance and feed utilisation of yellow catfish (P < 0·05). CDCA alleviated HF-induced increment of hepatic lipid and cholesterol contents by down-regulating the expressions of lipogenesis-related genes and proteins and up-regulating the expressions of lipololysis-related genes and proteins. Compared with the control group, CDCA group significantly reduced cholesterol level (P < 0·05). CDCA significantly inhibited BA biosynthesis and changed BA profile by activating farnesoid X receptor (P < 0·05). The contents of CDCA, taurochenodeoxycholic acid and glycochenodeoxycholic acid were significantly increased with the supplementation of CDCA (P < 0·05). HF-induced elevation of cholic acid content was significantly attenuated by the supplementation of CDCA (P < 0·05). Supplementation of CDCA in the control and HF groups could improve the liver antioxidant capacity. This study proved that CDCA could improve growth retardation, lipid accumulation and BA metabolism disorder induced by HF diet, which provided new insight into understanding the physiological functions of BA in fish.

Type
Research Article
Copyright
© The Author(s), 2023. Published by Cambridge University Press on behalf of The Nutrition Society

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