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Viability, growth and toxicity of Alexandrium catenella and Alexandrium minutum (Dinophyceae) following ingestion and gut passage in the oyster Crassostrea gigas

Published online by Cambridge University Press:  17 May 2007

Mohamed Laabir ,
Zouher Amzil ,
Patrick Lassus ,
Estelle Masseret ,
Yosmina Tapilatu ,
Romain De Vargas  and
Daniel Grzebyk
Mohamed Laabir*
Affiliation:
Laboratoire Ecosystèmes Lagunaires, UMR CNRS-UM2 5119 case 093, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier, France
Zouher Amzil
Affiliation:
Laboratoire Phycotoxines, IFREMER, Centre de Nantes, BP 21105, 44311 Nantes, France
Patrick Lassus
Affiliation:
Laboratoire Phycotoxines, IFREMER, Centre de Nantes, BP 21105, 44311 Nantes, France
Estelle Masseret
Affiliation:
Laboratoire Ecosystèmes Lagunaires, UMR CNRS-UM2 5119 case 093, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier, France
Yosmina Tapilatu
Affiliation:
Laboratoire Ecosystèmes Lagunaires, UMR CNRS-UM2 5119 case 093, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier, France
Romain De Vargas
Affiliation:
Laboratoire Ecosystèmes Lagunaires, UMR CNRS-UM2 5119 case 093, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier, France
Daniel Grzebyk
Affiliation:
Laboratoire Ecosystèmes Lagunaires, UMR CNRS-UM2 5119 case 093, Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier, France
*
laabir@univ-montp2.fr
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Abstract

Adult oysters Crassostrea gigas were experimentally fed with Alexandrium catenella and Alexandrium minutum which are responsible for recurrent toxic blooms in French coastal waters. C. gigas produced faeces and pseudofaeces containing intact and viable temporary pellicular cysts of these two Paralytic toxin producing species. When incubated in favourable conditions, these pellicular cysts were able to germinate at high rates (between 74 and 94%) and the resulting vegetative cells divided with growth rates close to the non- ingested cells (control). The toxin profile of the vegetative cells originated from the germinated temporary cysts was analyzed by liquid chromatography/fluorescence detection. Total toxin content of newly germinated cells was lower than that of cultured cells. Besides, cell contents of C2, B1, B2 and dcGTX3 toxins featured some changes. Our results suggest that the increased spreading of toxic dinoflagellates through the transfer of shellfish from contaminated towards pristine coastal areas cannot be ruled out. We also suggest that pellicular cysts and newly germinated cells could represent a potential way for the transfer of paralytic toxins toward the higher trophic levels.

Keywords

Alexandrium catenellaAlexandrium minutumCystsOystersParalytic Shellfish Poisoning
Type
Research Article
Information
Aquatic Living Resources , Volume 20 , Issue 1: New developments in coastal environment research: results from the national coastal environment program , January 2007 , pp. 51 - 57
Copyright
© EDP Sciences, IFREMER, IRD, 2007

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