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Short-term storage and cryopreservation of turbot (Scophthalmus maximus) sperm

Published online by Cambridge University Press:  15 July 1997

Olvido Chereguini ,
Rosa Maria Cal ,
Catherine Dreanno ,
Bénédicte Ogier de Baulny ,
Marc Suquet  and
Gérard Maisse
Olvido Chereguini
Affiliation:
I.E.O., Centro Oceanográfico de Santander, Apdo n 240, 39080 Santander, Spain
Rosa Maria Cal
Affiliation:
I.E.O., Centro Oceanográfico de Vigo, Apdo n 1552, 36280 Vigo, Spain
Catherine Dreanno
Affiliation:
IFREMER, Laboratoire de Physiologie des Poissons, Centre de Brest, BP 70, 29280 Plouzané, France
Bénédicte Ogier de Baulny
Affiliation:
INRA, Unité de Physiologie des Poissons, IFR 43, Campus de Beaulieu, 35042 Rennes Cedex, France
Marc Suquet
Affiliation:
IFREMER, Laboratoire de Physiologie des Poissons, Centre de Brest, BP 70, 29280 Plouzané, France
Gérard Maisse
Affiliation:
INRA, Unité de Physiologie des Poissons, IFR 43, Campus de Beaulieu, 35042 Rennes Cedex, France Author to whom correspondence should be addressed; E-mail: maisse@beaulieu.rennes.inra.fr
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Abstract

Short-term storage over several days as well as cryopreservation of turbot (Scophthalmus maximus) sperm were studied. Two extenders, Ringer 200 and artificial seminal liquid (ASL2), are suggested for semen collection in order to avoid the deleterious effect of urine contamination, and for the purpose of short-term storage between 0 and 15 °C. Oxygen atmosphere is not suitable for turbot sperm storage. Turbot spermatozoa undergo cryopreservation with a high rate of success especially in a sucrose solution with 10% dimethyl sulfoxide (DMSO) and 10% egg yolk.

Keywords

Turbotspermstoragecryopreservation
Type
Research Article
Information
Aquatic Living Resources , Volume 10 , Issue 4 , July 1997 , pp. 251 - 255
Copyright
© IFREMER-Gauthier-Villars, 1997

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