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Serological analysis of Bonamia in Ostrea edulis and Tiostrea lutaria using polyclonal and monoclonal antibodies

Published online by Cambridge University Press:  15 January 1988

Eric Mialhe ,
Viviane Boulo ,
Ralph Elston ,
Barry Hill ,
Michel Hine ,
Jaime Montes ,
Paul Van Banning  and
Henri Grizel
Eric Mialhe
Affiliation:
IFREMER, Laboratoire de pathologie et de génétique des Invertébrés marins, BP 133, 17390 La Tremblade, France
Viviane Boulo
Affiliation:
IFREMER, Laboratoire de pathologie et de génétique des Invertébrés marins, BP 133, 17390 La Tremblade, France
Ralph Elston
Affiliation:
Center for Marine Disease Control, Battelle Marine Research Laboratory, 439 West Sequim Bay Road, Sequim, WA 98382, USA
Barry Hill
Affiliation:
Ministry of Agriculture, Fisheries & Food Fish Diseases Laboratory Weymouth, Dorset, Great Britain
Michel Hine
Affiliation:
Fisheries Research Center, Ministry of Agriculture and Fisheries, PO Box 297, Wellington, New Zealand
Jaime Montes
Affiliation:
Centro Experimental Villajuan, Conseillera de Pesca, Apartado 208, Villagarcia, Pontevedra, Spain
Paul Van Banning
Affiliation:
Netherlands Institute for Fishery Investigations, PO Box 68, 1970 AB, Ijmuiden, Netherlands
Henri Grizel
Affiliation:
IFREMER, Laboratoire de pathologie et de génétique des Invertébrés marins, BP 133, 17390 La Tremblade, France
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Abstract

Murine monoclonal and polyclonal antibodies to antigens of Bonamia ostreae were assessed for serological comparison of parasites associated with different geographical origins of flat oysters, Ostrea edulis, and with Tiostrea lutaria. All Bonamia isolates from O. edulis reacted similary with polyclonal and monoclonal antibodies. The parasite associated with Tiostrea lutaria which lacks reactivity with 20 B2 MAB must be considered as a serologically distinct form of B. ostreae.

Keywords

BonamiaOstrea edulisTiostrea lutariapolyclonal and monoclonal antibodiesserological comparisonBonamiaOstrea edulisTiostrea lutariaanticorps polyclonaux et monoclonauxcomparaison sérologique
Type
Research Article
Information
Aquatic Living Resources , Volume 1 , Issue 1 , January 1988 , pp. 67 - 69
Copyright
© IFREMER-Gauthier-Villars, 1988

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