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Genotoxic and CYP 1A enzyme effects consecutive to the food transfer of oil spill contaminants from mussels to mammals

Published online by Cambridge University Press:  15 October 2004

Sébastien Lemière ,
Carole Cossu-Leguille ,
Sylvie Chaty ,
François Rodius ,
Antonio Bispo ,
Marie-José Jourdain ,
Marie-Claire Lanhers ,
Daniel Burnel  and
Paule Vasseur
Sébastien Lemière
Affiliation:
ESE, CNRS FRE 2635, Université de Metz, UFR SciFA, Av. Delestraint, 57070 Metz, France
Carole Cossu-Leguille
Affiliation:
ESE, CNRS FRE 2635, Université de Metz, UFR SciFA, Av. Delestraint, 57070 Metz, France
Sylvie Chaty
Affiliation:
ESE, CNRS FRE 2635, Université de Metz, UFR SciFA, Av. Delestraint, 57070 Metz, France
François Rodius
Affiliation:
ESE, CNRS FRE 2635, Université de Metz, UFR SciFA, Av. Delestraint, 57070 Metz, France
Antonio Bispo
Affiliation:
IRH Environnement, 11 bis rue G. Peri, BP 286, 54515 Vandœuvre-les-Nancy, France
Marie-José Jourdain
Affiliation:
IRH Environnement, 11 bis rue G. Peri, BP 286, 54515 Vandœuvre-les-Nancy, France
Marie-Claire Lanhers
Affiliation:
INSERM U420, Faculté de Médecine, 9 Av. Forêt de Haye, 54505 Vandœuvre-les-Nancy, France
Daniel Burnel
Affiliation:
INSERM U420, Faculté de Médecine, 9 Av. Forêt de Haye, 54505 Vandœuvre-les-Nancy, France
Paule Vasseur
Affiliation:
ESE, CNRS FRE 2635, Université de Metz, UFR SciFA, Av. Delestraint, 57070 Metz, France
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Abstract

The transfer of polycyclic aromatic hydrocarbons (PAHs) from bivalves, through the food chain to vertebrates was of concern. Our research aimed at estimating potential effects for consumers resulting from the ingestion of seafood contaminated by oil spill pollutants. After the “Erika” wreck, mussels (Mytilus sp.) were collected from sites of the Atlantic coast impacted to various degrees by the oil slick and constituted contaminated food for rats during 2 and 4 weeks. Genotoxic damage were studied in rats by means of COMET assay carried out in liver, bone marrow and peripheral blood. Biochemical and genomic effects such as the induction of CYP 1A1 and the expression of cytochrome genes were measured in rat livers. The most sensitive biological parameter reflecting the transfer of contaminants via the food appeared to be DNA breaks studied by means of the COMET assay. Genotoxic damage, observed mainly in the liver, were rather moderate and remained not persistent. This study underlined the bioavailability of pollutants in fuel oil contaminated mussels for consumers, and the complexity of the contamination consecutive to the oil spill. The occurrence of related PAH compounds in addition to non-substituted PAHs in fuel oils and mussels raised the question of their implication in the registered effects.

Keywords

Polycyclic aromatic hydrocarbonsOil spillCOMET assayDNA damageEROD activityCYP 1AFood transfer
Type
Research Article
Information
Aquatic Living Resources , Volume 17 , Issue 3: The "Erika" Oil Spill: Environmental Contamination and Effects in the Bay of Biscay , July 2004 , pp. 303 - 307
Copyright
© EDP Sciences, IFREMER, IRD, 2004

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References

AFNOR, 2001, Qualité de l'eau. Méthode de mesure de l'activité enzymatique EROD chez le poisson d'eaux douces et marines. NF T 90-385, Association Française de Normalisation, Paris, France.
AFSSA, 2000, Avis du groupe d'experts réunis par l'Agence française de sécurité sanitaire des aliments sur les critères de toxicité alimentaire présentés par la pollution engendrée par le naufrage de l'Erika. Agence Française de Sécurité Sanitaire des Aliments, Maisons-Alfort, France.
Bradford, M.M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal. Biochem. 72, 248-254. CrossRef
Borosky, G.L., 1999, Theoretical study related to the carcinogenic activity of polycyclic aromatic hydrocarbon derivatives. J. Org. Chem. 64, 7738-7744. CrossRef
Burke, M.D., Mayer, R.T., 1974, Ethoxyresorufin: direct fluorimetric assay of a microsomal O-dealkylate which is preferentially inducible by 3-methylcholanthrene. Drug Metab. Dispos. 2, 583-588.
Chaty S., 2003, Effets d'inducteurs-types sur l'expression du cytochrome P450 chez les bivalves après exposition directe et chez les mammifères contaminés par la voie alimentaire. Thèse de doctorat, ESE, Toxicologie, Université de Metz.
Christmann, M., Tomicic, M.J., Roos, W.P., Kaina, B., 2003, Mechanisms of human DNA repair: an update. Toxicology 193, 3-34. CrossRef
Fernley, P.W., Moore, M.N., Lowe, D.M., Donkin, P., Evans, S., 2000, Impact of the “Sea Empress” oil spill on lysosomal stability in mussel blood cells. Mar. Environ. Res. 50, 451-455. CrossRef
Gilroy, D.J., 2000, Derivation of shellfish harvest reopening criteria following the “New Carissa” oil spill in Coos Bay, Oregon. J. Toxicol. Environ. Health A 60, 317-329. CrossRef
Glegg, G.A., Hickman, L., Rowland, S.J., 1999, Contamination of limpets (Patella vulgata) following the “Sea Empress” oil spill. Mar. Pollut. Bull. 38, 119-125. CrossRef
Harvey, J.S., Lyons, B.P., Page, T.S., Stewart, C., Parry, J.M., 1999, An assessment of the genotoxic impact of the “Sea Empress” oil spill by the measurement of DNA adduct levels in selected invertebrate and vertebrate species. Mutat. Res. 441, 103-114. CrossRef
IARC, 1987, Monogaph on the evaluation of carcinogenic risks of chemicals to humans. Overall evaluations of carcinogenicity: An updating of IARC Monographs, Vol. 1 to 42. International Agency for Research on Cancer, Lyon, France.
Law, R.J., Kelly, C.A., Baker, K.L., Langford, K.H., Bartlett, T., 2002, Polycyclic aromatic hydrocarbons in sediments, mussels and crustacea around a former gasworks site in Shoreham-by-Sea, UK. Mar. Pollut. Bull. 44, 903-911. CrossRef
Meador, J.P., Stein, J.E., Reichert, W.L., Varanasi, U., 1995, Bioaccumulation of polycyclic aromatic hydrocarbons by marine organisms. Rev. Environ. Contam. Toxicol. 143, 79-165.
Nordqvist, M., Thakker, D.R., Levin, W., Yagi, H., Ryan, D.E., Thomas, P.E., Cooney, A.H., Jerina, D.M., 1979, The highly tumorigenic 3,4-dihydrodiol is a principal metabolite formed from dibenzo(a,h) anthracene by liver enzymes. Mol. Pharmacol. 16, 643-655.
Nunn J.W., Livingstone D.R., Chipman J.K., 1996, Effect of genetic toxicants in aquatic organisms. In: Taylor E.W. (Eds), Toxicology and Aquatic Pollution: Physiological, Molecular and Cellular Approaches 57, Cambridge University Press, Cambridge, pp. 225–251.
Office of the Federal Register (OFR), 1982, Appendix A: Priority Pollutants. Fed. Reg. 47, 52309.
Poon, R., Davis, H., Lecavalier, P., Liteplo, R., Yagminas, A., Chu, I., Bihun, C.J., 1997, Effects of benzothiophene on male rats following short-term oral exposure. J. Toxicol. Environ. Health 50, 53-65.
Poon, R., Davis, H., Park, G., Lecavalier, P., Yagminas, A., Elwin, J., Chu, I., Valli, V.E., 1998, Subchronic toxicity of benzothiophene on rats following dietary exposure. J. Toxicol. Environ. Health A 55, 133-150.
Sarasin, A., 2003, An overview of the mechanisms of mutagenesis and carcinogenesis. Mutat. Res. 544, 99-106. CrossRef
Singh, N.P., McCoy, M.T., Tice, R.R., Schneider, E.L., 1988, A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175, 184-191. CrossRef
Sinsheimer, J.E., Hooberman, B.H., Das, S.K., Savla, P.M., Ashe, A.J., 1992, Genotoxicity of chryseno[4,5-bcd] thiophene and its sulfone derivative. Environ. Mol. Mutagen. 19, 259-264. CrossRef
Solé, M., Porte, C., Biosca, X., Mitchelmore, C.L., Chipman, J.K., Livingstone, D.R., Albaigés, J., 1996, Effects of the “Aegean Sea” oil spill on biotransformation enzymes, oxidative stress and DNA-adducts in digestive gland of the mussel (Mytilus edulis L.). Comp. Biochem. Physiol. C, Pharmacol. Toxicol. Endocrinol. 113, 257-265.
Thomas, R.E., Brodersen, C., Carls, M.G., Babcock, M., Rice, S.D., 1999a, Lack of physiological responses to hydrocarbon accumulation by Mytilus trossulus after 3–4 years chronic exposure to spilled “Exxon Valdez” crude oil in Prince William Sound. Comp. Biochem. Physiol. C, Pharmacol. Toxicol. Endocrinol. 122, 153-163.
Thomas, R.E., Harris, P.M., Rice, S.D., 1999b, Survival in air of Mytilus trossulus following long-term exposure to spilled “Exxon Valdez” crude oil in Prince William sound. Comp. Biochem. Physiol. C, Pharmacol. Toxicol. Endocrinol. 122, 147-152.