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Comparative study of shell shape and muscle scar pigmentation in the closely related cupped oysters Crassostrea angulata, C. gigas and their reciprocal hybrids

Published online by Cambridge University Press:  05 April 2008

Frederico M. Batista ,
Radhouan Ben-Hamadou ,
Vera G. Fonseca ,
Nicolas Taris ,
Francisco Ruano ,
Maria A. Reis-Henriques  and
Pierre Boudry
Frederico M. Batista
Affiliation:
Instituto Nacional de Investigação Agrária e das Pescas (INIAP/IPIMAR), CRIPSul, Av. 5 de Outubro, 8700-305 Olhão, Portugal Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal
Radhouan Ben-Hamadou
Affiliation:
Centro de Ciências do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
Vera G. Fonseca
Affiliation:
Centro de Ciências do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire de Génétique et Pathologie (LGP), 17390 La Tremblade, France
Nicolas Taris
Affiliation:
Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire de Génétique et Pathologie (LGP), 17390 La Tremblade, France
Francisco Ruano
Affiliation:
Instituto Nacional de Investigação Agrária e das Pescas (INIAP/IPIMAR), Departamento de Aquicultura, Av. de Brasília, 1449-006 Lisboa, Portugal
Maria A. Reis-Henriques
Affiliation:
Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal
Pierre Boudry
Affiliation:
Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire de Génétique et Pathologie (LGP), 17390 La Tremblade, France
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Abstract

The taxonomic status of the cupped oysters Crassostrea angulata and C. gigas has received considerable attention in the last decades. Based on larval shell morphology, experimental hybridization, allozymes and nuclear DNA studies several authors have considered these two taxa as being synonymous. However, mitochondrial data showed clear genetic differences between the two taxa. In addition, microsatellite-based studies and cytogenetic studies have also provided evidence that supports their differentiation. Considerable differences have also been observed at the phenotypic level in terms of growth rate and ecophysiological parameters. In the present study, C. angulata from Sado estuary (Portugal) and C. gigas from Seudre estuary (France) were collected and factorial crosses were performed. Juveniles of the different progenies were reared in Ria Formosa (Portugal) under common conditions to determine if they exhibited differences in shell shape and in pigmentation of the adductor muscle scar. Significant morphometric differences between C. angulata and C. gigas progenies were indicated by univariate and multivariate analyses. Univariate analysis of size-adjusted shell measurements revealed significant differences between the two taxa for shell depth, muscle scar height, and length of ligamental area. Both reciprocal hybrids showed intermediate morphometric characters between parental lines. In addition, significant differences were also observed between C. angulata and C. gigas progenies in terms of pigmentation of adductor muscle scar. C. angulata and both reciprocal hybrid progenies showed highly pigmented adductor muscle scars whereas in C. gigas progeny the pigmentation was lighter. The differences in shell shape and muscle scar pigmentation observed in the present study support the distinction of the two taxa.

Keywords

Shell morphologyShell pigmentationHybridizationCrassostrea angulataCrassostrea gigas
Type
Research Article
Information
Aquatic Living Resources , Volume 21 , Issue 1 , January 2008 , pp. 31 - 38
Copyright
© EDP Sciences, IFREMER, IRD, 2008

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