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The role of zinc chelate of hydroxy analogue of methionine in cadmium toxicity: effects on cadmium absorption on intestinal health in piglets

Published online by Cambridge University Press:  13 February 2020

H. J. Ni Open the ORCID record for H. J. Ni [Opens in a new window] ,
F. F. Liu ,
X. Liang ,
Y.L. Yin  and
G. Liu
H. J. Ni
Affiliation:
National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan410125, P. R. China
F. F. Liu
Affiliation:
National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan410125, P. R. China Department of Animal Science, Hunan Agriculture University, Changsha, Hunan410125, P. R. China
X. Liang
Affiliation:
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong266109, P. R. China
Y.L. Yin
Affiliation:
National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan410125, P. R. China Department of Animal Science, Hunan Agriculture University, Changsha, Hunan410125, P. R. China
G. Liu*
Affiliation:
National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan410125, P. R. China
*
†E-mail: gangle.liu@gmail.com
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Abstract

Cadmium (Cd) is a toxic heavy metal that poses a threat to the health of humans and animals. It can cause serious damage to the small intestine, which is the main absorption site of Cd and the primary target organ after oral administration. Our previous study found that zinc chelate of hydroxy analogue of methionine (Zn-HMTB), a new type of feed additive, decreased Cd accumulation in the liver and kidneys. The aim of this study was to investigate the effect of Zn-HMTB on Cd absorption and Cd-induced toxicity in the small intestine of piglets. Twenty-four piglets (Landrace × Large White, 13.22 ± 0.58 kg BW) were randomly divided into four dietary treatment groups: basal diet, and diets containing 30 mg/kg Cd from CdCl2 and 0, 100 or 200 mg/kg Zn from Zn-HMTB. The experiment lasted 27 days. The feed intake and final BW of each piglet were recorded at the end of the experiment. Gastrointestinal (GI) tract tissue and samples of liver, kidney, spleen, heart, lung and longissimus muscle tissue and faeces were collected. The concentrations of Cd and metal trace elements in the GI tract and organs were analysed, as was the relative messenger RNA (mRNA) expression of inflammatory cytokines and metal element transporters in the small intestine, and epithelial apoptosis in the small intestine. The results showed that, compared with Cd-treated piglets, piglets in the Zn-HMTB and Cd cotreatment groups had less Cd deposition in the stomach, ileum, caecum, colon, liver, kidneys, spleen, lungs, heart and muscles (P < 0.05), and lower Cd concentrations in faeces (P < 0.05), suggesting that Zn-HMTB increased Cd absorption and the excretion of Cd in other forms (possibly urine). Zinc chelate of hydroxy analogue of methionine increased Zn deposition in the jejunum and the relative mRNA expression of divalent metal transporters 1 and zinc transporter 5 in the duodenum (P < 0.05), indicating that Zn-HMTB may promote the absorption and transportation of Cd and Zn together by upregulating metal element transporters. Competition between Zn and Cd may be responsible for accelerating Cd excretion. Furthermore, Zn-HMTB reduced Cd-induced apoptosis of enterocytes and inflammatory stimuli in the small intestine, suggesting that Zn-HMTB reduced Cd-induced toxicity to the small intestine. These results suggest that Zn-HMTB can be helpful in decreasing Cd accumulation in the GI tract and organs of piglets and relieving Cd-induced toxicity to the small intestine but cannot reduce the absorption of Cd.

Keywords

pigletcadmiumzincsmall intestinemetal absorption
Type
Research Article
Information
animal , Volume 14 , Issue 7 , July 2020 , pp. 1382 - 1391
Copyright
© The Animal Consortium 2020

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Footnotes

*

These authors contributed equally to this work.

References

Association of Official Analytical Chemists (AOAC ) 2004. Official methods of analysis, volume 2, 18th edition. AOAC, Arlington, VA, USA.Google Scholar
Ben Mimouna, S, Chemek, M, Boughammoura, S, Haouas, Z and Messaoudi, I 2018. Protective role of zinc against the neurotoxicity induced by exposure to cadmium during gestation and lactation periods on hippocampal volume of pups tested in early adulthood. Drug and Chemical Toxicology 41, 424–433.CrossRefGoogle ScholarPubMed
Bernhard, D, Rossmann, A, Henderson, B, Kind, M, Seubert, A and Wick, G 2006. Increased serum cadmium and strontium levels in young smokers: effects on arterial endothelial cell gene transcription. Arteriosclerosis, Thrombosis, and Vascular Biology 26, 833–838.CrossRefGoogle ScholarPubMed
Boveri, M, Pazos, P, Gennari, A, Casado, J, Hartung, T and Prieto, P 2004. Comparison of the sensitivity of different toxicological endpoints in Caco-2 cells after cadmium chloride treatment. Archives of Toxicology 78, 201–206.CrossRefGoogle ScholarPubMed
Colovic, M, Vasic, V, Djuric, D and Krstic, D 2018. Sulphur-containing amino acids: protective role against free radicals and heavy metals. Current Medicinal Chemistry 25, 324–335.CrossRefGoogle ScholarPubMed
Deng, B, Zhou, X, Wu, J, Long, C, Yao, Y, Peng, H, Wan, D and Wu, X 2017. Effects of dietary supplementation with tribasic zinc sulfate or zinc sulfate on growth performance, zinc content and expression of zinc transporters in young pigs. Animal Science Journal 88, 1556–1560.CrossRefGoogle ScholarPubMed
Duizer, E, Gilde, AJ, Versantvoort, CHM and Groten, JP 1999. Effects of cadmium chloride on the paracellular barrier function of intestinal epithelial cell lines. Toxicology and Applied Pharmacology 155, 117–126.CrossRefGoogle ScholarPubMed
Erdem, O, Yazihan, N, Kocak, MK, Sayal, A and Akcil, E 2016. Influence of chronic cadmium exposure on the tissue distribution of copper and zinc and oxidative stress parameters in rats. Toxicology and Industrial Health 32, 1505–1514.CrossRefGoogle ScholarPubMed
Govind, P and Madhuri, S 2014. Heavy metals causing toxicity in animals and fishes. Research Journal of Animal, Veterinary and Fishery Sciences 2, 17–23.Google Scholar
Hejna, M, Gottardo, D, Baldi, A, Dell’Orto, V, Cheli, F, Zaninelli, M and Rossi, L 2018. Review: Nutritional ecology of heavy metals. Animal 12, 2156–2170.CrossRefGoogle ScholarPubMed
Hoogenboom, RLAP, Hattink, J, van Polanen, A, van Oostrom, S, Verbunt, JT, Traag, WA, Kan, KA, van Eijkeren, JCH, De Boeck, G and Zeilmaker, MJ 2015. Carryover of cadmium from feed in growing pigs. Food Additives & Contaminants: Part A 32, 68–79.CrossRefGoogle ScholarPubMed
Hyun, JS, Satsu, H and Shimizu, M 2007. Cadmium induces Interleukin-8 production via NF-κB activation in the human intestinal epithelial cell, Caco-2. Cytokine 37, 26–34.CrossRefGoogle ScholarPubMed
Jin, T, Nordberg, GF, Ye, T, Bo, M, Wang, H, Zhu, G, Kong, Q and Bernard, A 2004. Osteoporosis and renal dysfunction in a general population exposed to cadmium in China. Environmental Research 96, 353–359.CrossRefGoogle Scholar
Kaewtapee, C, Krutthai, N and Bunchasak, C 2016. Effects of supplemental liquid dl-methionine hydroxy analog free acid in diet on growth performance and gastrointestinal functions of piglets. Asian-Australasian Journal of Animal Sciences 29, 1166–1172.CrossRefGoogle ScholarPubMed
Lag, M, Rodionov, DG, Ovrevik, J, Bakke, O, Schwarze, PE and Refsnes, M 2010. Cadmium-induced inflammatory responses in cells relevant for lung toxicity: expression and release of cytokines in fibroblasts, epithelial cells and macrophages. Toxicology Letters 193, 252–260.CrossRefGoogle ScholarPubMed
Liu, F, Ni, H, Huang, P, Wu, X, Zhang, B, Zhang, W, Yao, Y and Yin, Y 2017. The protective effect of methionine hydroxy analog chelated zinc on cadmium induced damage in weaned piglets. Acta Veterinaria et Zootechnica Sinica 49, 318–326.Google Scholar
Liu, Y, Li, Y, Liu, K and Shen, J 2014. Exposing to cadmium stress cause profound toxic effect on microbiota of the mice intestinal tract. PLoS ONE 9, e85323.CrossRefGoogle ScholarPubMed
Martín-Venegas, R, Teresa Brufau, M, Mercier, Y, Geraert, P-A and Ferrer, R 2011. Intestinal cell conversion of dl-2-hydroxy-(4-methylthio)butanoic acid in vitro: dietary up-regulation by this methionine precursor. British Journal of Nutrition 106, 350–356.CrossRefGoogle ScholarPubMed
National Research Council (NRC ) 2012. Nutrient requirements of swine, 11th revisededition. National Academy Press, Washington, DC, USA.Google Scholar
Newbigging, AM, Yan, X and Le, XC 2015. Cadmium in soybeans and the relevance to human exposure. Journal of Environmental Sciences-China 37, 157–162.CrossRefGoogle ScholarPubMed
Ninkov, M, Popov Aleksandrov, A, Demenesku, J, Mirkov, I, Mileusnic, D, Petrovic, A, Grigorov, I, Zolotarevski, L, Tolinacki, M, Kataranovski, D, Brceski, I and Kataranovski, M 2015. Toxicity of oral cadmium intake: impact on gut immunity. Toxicology Letters 237, 89–99.CrossRefGoogle ScholarPubMed
Nordberg, GF, Bernard, A, Diamond, GL, Duffus, JH, Illing, P, Nordberg, M, Bergdahl, IA, Jin, T and Skerfving, S 2018. Risk assessment of effects of cadmium on human health (IUPAC Technical Report). Pure and Applied Chemistry 90, 755–808.CrossRefGoogle Scholar
Olszowski, T, Baranowskabosiacka, I, Gutowska, I and Chlubek, D 2012. Pro-inflammatory properties of cadmium. Acta Biochimica Polonica 59, 475–482.CrossRefGoogle ScholarPubMed
Powell, CD, Chowdhury, MAK and Bureau, DP 2017. Assessing the bioavailability of l-methionine and a methionine hydroxy analogue (MHA-Ca) compared to dl-methionine in rainbow trout (Oncorhynchus mykiss). Aquaculture Research 48, 332–346.CrossRefGoogle Scholar
Roth, JA and Salvi, R 2016. Ototoxicity of divalent metals. Neurotoxicity Research 30, 268–282.CrossRefGoogle ScholarPubMed
Salami, SA, Oluwatosin, OO, Oso, AO, Fafiolu, AO, Sogunle, OM, Jegede, AV, Bello, FA and Pirgozliev, V 2016. Bioavailability of Cu, Zn and Mn from mineral chelates or blends of inorganic salts in growing Turkeys fed with supplemental riboflavin and/or pyridoxine. Biological Trace Element Research 173, 168–176.CrossRefGoogle ScholarPubMed
Thévenod, F and Lee, W-K 2013. Toxicology of cadmium and its damage to mammalian organs. In Cadmium: from toxicity to essentiality (ed. Sigel, A, Sigel, H and Sigel, RKO), pp. 415–490. Springer Publishing, Dordrecht, Netherlands.CrossRefGoogle Scholar
Vance, TM and Chun, OK 2015. Zinc intake is associated with lower cadmium burden in US adults. The Journal of Nutrition 145, 2741–2748.CrossRefGoogle Scholar
Vesey, DA 2010. Transport pathways for cadmium in the intestine and kidney proximal tubule: focus on the interaction with essential metals. Toxicology Letters 198, 13–19.CrossRefGoogle ScholarPubMed
Wang, X and Zhou, B 2010. Dietary zinc absorption: a play of zips and ZnTs in the gut. Iubmb Life 62, 176–182.CrossRefGoogle Scholar
World Health Organization (WHO) 1992. Cadmium, environmental health criteria 134. WHO, Geneva, Switzerland.Google Scholar
Yabe, J, Nakayama, SMM, Ikenaka, Y, Yohannes, YB, Bortey-Sam, N, Kabalo, AN, Ntapisha, J, Mizukawa, H, Umemura, T and Ishizuka, M 2018. Lead and cadmium excretion in feces and urine of children from polluted townships near a lead-zinc mine in Kabwe, Zambia. Chemosphere 202, 48–55.CrossRefGoogle Scholar
Zhou, X, Zhang, Y, He, L, Wan, D, Liu, G, Wu, X and Yin, Y 2017. Serine prevents LPS-induced intestinal inflammation and barrier damage via p53-dependent glutathione synthesis and AMPK activation. Journal of Functional Foods 39, 225–232.CrossRefGoogle Scholar
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