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Polymorphism of the caprine malic enzyme 1 (ME1) gene and its association with milk quality traits in Murciano–Granadina goats

Published online by Cambridge University Press:  10 June 2010

A. Zidi ,
J. M. Serradilla ,
J. Jordana ,
J. Carrizosa ,
B. Urrutia ,
O. Polvillo ,
P. González-Redondo ,
D. Gallardo ,
M. Amills  and
V. M. Fernández-Cabanás
A. Zidi
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
J. M. Serradilla
Affiliation:
Departamento de Producción Animal, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain
J. Jordana
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
J. Carrizosa
Affiliation:
Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), Estación Sericícola. La Alberca, Murcia, Spain
B. Urrutia
Affiliation:
Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), Estación Sericícola. La Alberca, Murcia, Spain
O. Polvillo
Affiliation:
Departamento de Ciencias Agroforestales, Escuela Universitaria de Ingeniería Técnica Agrícola-Universidad de Sevilla, Carretera Utrera, km. 1. 41013 Sevilla, Sevilla, Spain
P. González-Redondo
Affiliation:
Departamento de Ciencias Agroforestales, Escuela Universitaria de Ingeniería Técnica Agrícola-Universidad de Sevilla, Carretera Utrera, km. 1. 41013 Sevilla, Sevilla, Spain
D. Gallardo
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
M. Amills*
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
V. M. Fernández-Cabanás*
Affiliation:
Departamento de Ciencias Agroforestales, Escuela Universitaria de Ingeniería Técnica Agrícola-Universidad de Sevilla, Carretera Utrera, km. 1. 41013 Sevilla, Sevilla, Spain
*
†E-mail: Marcel.Amills@uab.cat, victorf@us.es
†E-mail: Marcel.Amills@uab.cat, victorf@us.es
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Abstract

Malic enzyme 1 plays a fundamental role in lipid metabolism because it yields a significant amount of the NADPH necessary for fatty acid biosynthesis. In ruminants, however, its contribution to this biochemical process seems to be less relevant than in other livestock species. In this study, we have partially sequenced the goat ME1 gene with the aim of identifying polymorphic sites. Structural analysis of the goat ME1 amino acid partial sequence evidenced the existence of two dinucleotide-binding motifs, located at positions 158 to 163 (GLGDLG) and 301 to 306 (GAGEAA), and three amino acid residues (E245, D246 and D269) involved in the binding of Mn2+ and strongly conserved among mammalian species. Moreover, multiple sequence alignment allowed us to identify four single nucleotide polymorphisms at exons 5 (c.483C > T), 6 (c.667G > A), 9 (c.927C > T) and 11 (c.1200G > A). The effects of ME1 genotype on milk production and composition traits were investigated in a Murciano–Granadina goat population. None of the associations found reached statistical significance after applying Benjamini–Hochberg correction. At most, associations with uncorrected P-values below 0.01 were observed for C16:0, C18:1n-9t and total conjugated linoleic acids. These negative results reinforce the notion that ME1 plays an ancillary role in ruminant lipogenesis.

Keywords

goatmalic enzymegenotypefatty acidsmilk composition
Type
Full Paper
Information
animal , Volume 4 , Issue 12 , December 2010 , pp. 1953 - 1957
Copyright
Copyright © The Animal Consortium 2010

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