Hostname: page-component-77c89778f8-rkxrd Total loading time: 0 Render date: 2024-07-17T16:06:02.319Z Has data issue: false hasContentIssue false
  • English
  • Français

Liver metabolism traits in two rabbit lines divergently selected for intramuscular fat

Published online by Cambridge University Press:  26 October 2017

M. Martínez-Álvaro ,
Y. Paucar ,
K. Satué ,
A. Blasco  and
P. Hernández
M. Martínez-Álvaro
Affiliation:
Institute for Animal Science and Technology, Universitat Politècnica de València, 46022 Valencia, Spain
Y. Paucar
Affiliation:
Institute for Animal Science and Technology, Universitat Politècnica de València, 46022 Valencia, Spain
K. Satué
Affiliation:
Department of Animal Medicine and Surgery, Universidad Cardenal Herrera, 46113 Valencia, Spain
A. Blasco
Affiliation:
Institute for Animal Science and Technology, Universitat Politècnica de València, 46022 Valencia, Spain
P. Hernández*
Affiliation:
Institute for Animal Science and Technology, Universitat Politècnica de València, 46022 Valencia, Spain
*
†E-mail: phernan@dca.upv.es
Get access

Abstract

Intramuscular fat (IMF) has a large effect in the sensory properties of meat because it affects tenderness, juiciness and flavour. A divergent selection experiment for IMF in longissimus dorsi (LD) muscle was performed in rabbits. Since liver is the major site of lipogenesis in rabbits, the objective of this work is to study the liver metabolism in the lines of the divergent selection experiment. Intramuscular fat content, perirenal fat weight, liver weight, liver lipogenic activities and plasma metabolites related to liver metabolism were measured in the eighth generation of selection. Direct response on IMF was 0.34 g/100 g of LD, which represented 2.7 SD of the trait, and selection showed a positive correlated response in the perirenal fat weight. High-IMF line showed greater liver size and greater liver lipogenic activities of enzymes glucose-6-phosphate dehydrogenase and malic enzyme. We did not find differences between lines for fatty acid synthase lipogenic activity. With regard to plasma metabolites, low-IMF line showed greater plasma concentration of triglycerides, cholesterol, bilirubin and alkaline phosphatase than high-IMF line, whereas high-IMF line showed greater albumin and alanine transaminase concentrations than low-IMF line. We did not observe differences between lines for glucose, total protein and plasma concentrations. Phenotypic correlations between fat (IMF and perirenal fat weight) and liver traits showed that liver lipogenesis affects fat deposition in both, muscle and carcass. However, the mechanisms whereby liver lipogenesis affected IMF content remain to be clarified.

Keywords

intramuscular fatlivermetabolismgenetic selectionrabbits
Type
Research Article
Information
animal , Volume 12 , Issue 6 , June 2018 , pp. 1217 - 1223
Copyright
© The Animal Consortium 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adachi, K, Kawano, H, Tsuno, K, Nomura, Y, Yamamoto, N, Arikawa, A, Tsuji, A, Adachi, M, Onimaru, T and Ohwada, K 1999. Relationship between serum biochemical values and marbling scores in Japanese Black steers. Journal of Veterinary Medical Science 61, 961–964.Google Scholar
Allen, CE, Beitz, DC, Cramer, DA and Kauffman, RG 1976. Biology of fat in meat animals. North Central Regional Research Publication No 234. University of Wisconsin, Madison, USA.Google Scholar
Bakke, H 1975. Serum levels of cholesterol in lines of pigs selected for rate of gain and thickness of backfat. Acta Agriculture Scandinavica 25, 14–16.CrossRefGoogle Scholar
Ballard, FJ, Hanson, RW and Kronfield, DS 1969. Gluconeogenesis and lipogenesis in tissue from ruminant and non-ruminant animals. Federation Proceedings 28, 218–231.Google Scholar
Blasco, A 2017. Bayesian analysis for animal scientists. Springer, New York, NY, USA.Google Scholar
Blasco, A and Ouhayoun, J 1996. Harmonization of criteria and terminology in rabbit meat research. Revised proposal. World Rabbit Science 4, 93–99.Google Scholar
Ciobanu, DC, Lonergan, SM and Huff-Lonergan, EJ 2011. Genetics of meat quality and carcass traits. In The genetics of the pig (ed. MF Rothschild and A Ruvinsky), pp. 355–389. CABI Publishing, Oxfordshire, UK.Google Scholar
Cui, HX, Zheng, MQ, Liu, RR, Zhao, GP, Chen, JL and Wen, J 2012. Liver dominant expression of fatty acid synthase (FAS) gene in two chicken breeds during intramuscular-fat development. Molecular Biology Reports 39, 3479–3484.CrossRefGoogle ScholarPubMed
European Commission Directive 2010. Council, E. P. A. E. 2010/63/ EU on the protection of animals used for scientific purposes. Institute for Health and Consumer Protection, Ispra, Italy, b7.Google Scholar
Frayn, KN 1998. Regulación del metabolismo: una perspectiva humana. Omega, Barcelona, Spain.Google Scholar
Gondret, F, Hocquette, JF and Herpin, P 2004. Age-related relationships between muscle fat content and metabolic traits in growing rabbits. Reproduction Nutrition Development 44, 1–16.Google Scholar
Gondret, F, Mourot, J and Bonneau, M 1997. Developmental changes in lipogenic enzymes in muscle compared to liver and extramuscular adipose tissues in the rabbit (oryctolagus cuniculus). Biochemistry and Molecular Biology 117B, 259–265.Google Scholar
Hernández, P, Ariño, B, Grimal, A and Blasco, A 2006. Comparison of carcass and meat characteristics of three rabbit lines selected for litter size or growth rate. Meat Science 73, 645–650.Google Scholar
Hernández, P, Pla, M, Oliver, MA and Blasco, A 2000. Relationships between meta quality mesurements in rabbits fed with three diets of different fat type and content. Meat Science 55, 379–384.Google Scholar
Jenko-Praznikar, Z, Petelin, A, Jurdana, M and Ziberna, L 2013. Serum bilirubin levels are lower in overweight asymptomatic middle-aged adults: an early indicator of metabolic syndrome? Metabolism Clinical and Experimental 62, 976–985.Google Scholar
Kaplan, LA, Pesce, AJ and Kazmierczak, SC 2009. Clinical chemistry: theory, analysis, correlation, 5th edition. C.V. Mosby, Toronto, Canada.Google Scholar
Lakshmanan, MR, Muesing, RA, Cook, GA and Veech, RL 1977. Regulation of lipogenesis in isolated hepatocytes by triglyceride-rich lipoproteins. Journal of Biological Chemistry 252, 6581–6584.Google Scholar
Legarra, A, Varona, L and López de Maturana, E 2008. TM: Thershold model. GenoToul Bioinformatics, Tolouse, France. Retrieved on 7 May 2017 from http://genoweb.toulouse.inra.fr/~alegarra/tm_folder/ Google Scholar
Martínez-Álvaro, M, Agha, S, Blasco, A and Hernández, P 2017. Muscle lipid metabolism in two rabbit lines divergently selected for intramuscular fat. Journal of Animal Science 95, 2576–2584.Google Scholar
Martínez-Álvaro, M, Hernández, P and Blasco, A 2016. Divergent selection on intramuscular fat in rabbits: responses to selection and genetic parameters. Journal of Animal Science 94, 4993–5003.Google Scholar
Melillo, A 2007. Rabbit clinical pathology. Journal of Exotic Pet Medicine 16, 135–145.CrossRefGoogle ScholarPubMed
Muñoz, R, Estany, J, Tor, M and Doran, O 2013. Hepatic lipogenic enzyme expression in pigs affected by selection for decreased backfat thickness at constant intramuscular fat content. Meat Science 93, 746–751.CrossRefGoogle ScholarPubMed
Muñoz, R, Tor, M and Estany, J 2012. Relationship between blood lipid indicators and fat content and composition in Duroc pigs. Livestock Science 148, 95–102.Google Scholar
O’ Hea, EK and Leveille, GA 1969. Lipid biosynthesis and transport in the domestic chick (Gallus domesticus). Comparative Biochemistry and Physiology 30, 149–159.Google Scholar
Pond, WG, Insull, W, Mersmann, HJ, Wong, WW, Harris, KB, Cross, HR, Smith, EO, Heath, JP and Kömüves, LG 1992. Effect of dietary fat and cholesterol level on growing pigs selected for three generations for high or low serum cholesterol at age 56 days. Journal of Animal Science 70, 2462–2470.Google Scholar
Pond, WG, Su, DR and Mersmann, HJ 1997. Divergent concentrations of plasma metabolites in swine selected for seven generations for high or low plasma total cholesterol. Journal of Animal Science 75, 311–316.CrossRefGoogle ScholarPubMed
Sapp, RL, Bertrand, JK, Pringle, TD and Wilson, DE 2002. Effects of selection for ultrasound intramuscular fat percentage in Angus bulls on carcass traits of progeny. Journal of Animal Science 80, 2017–2022.Google Scholar
Schwab, CR, Baas, TJ, Stalder, KJ and Nettleton, D 2009. Results from six generations of selection for intramuscular fat in Duroc swine using real-time ultrasound. I. Direct and correlated phenotypic responses to selection. Journal of Animal Science 87, 2774–2780.Google Scholar
Smith, PA and Kaplan, ML 1980. Development of hepatic and adipose tissue lipogenesis in the fa/fa rat. International Journal of Biochemistry 11, 217–228.Google Scholar
Turkenkopf, IJ, Olsen, JL, Moray, L, Greenwood, MRC and Johnson, PR 1980. Hepatic lipogenesis in preobese Zucker rat (40910). Proceedings of the Society for experimental Biology and Medicine 164, 530–533.CrossRefGoogle Scholar
Wang, X, Roy Chowdhury, J and Roy Chowdhury, N 2006. Bilirubin metabolism: applied physiology. Current Paediatrics 16, 70–74.Google Scholar
Washington, IM and Van Hoosier, GV 2012. Clinical biochemistry and hematology. In The laboratory rabbit, guinea pig, hamster, and other rodents (ed. MA Suckow, KA Stevens and RP Wilson), pp. 57–116. Blackwell Publishing Professional, Ames, Iowa, USA.Google Scholar
Wise, T, Young, DL and Pond, WG 1993. Reproductive, endocrine and organ weight differences of swine selected for high or low serum cholesterol. Journal of Animal Science 71, 2732–2738.Google Scholar
Wood, JD, Enser, M, Fisher, AV, Nute, GR, Sheard, PR, Richardson, RI, Hughes, SI and Whittington, FM 2008. Fat deposition, fatty acid composition and meat quality: a review. Meat Science 78, 343–358.Google Scholar
Ying, W 2008. NAD+/NADH and NADP+/NADPH in cellular functions an cell death: regulation and biological consequences. Antioxidants & Redox Signaling 10, 179–206.CrossRefGoogle ScholarPubMed
Zhao, GP, Chen, JL, Zheng, MQ, Wen, J and Zhang, Y 2007. Correlated responses to selection for increased intramuscular fat in a Chinese quality chicken line. Poultry Science 86, 2309–2314.Google Scholar
Zomeño, C, Blasco, A and Hernández, P 2010. Influence of genetic line on lipid metabolism traits of rabbit muscle. Journal of Animal Science 88, 3419–3427.Google Scholar
Zomeño, C, Hernández, P and Blasco, A 2011. Use of near infrared spectroscopy for intramuscular fat selection in rabbits. World Rabbit Science 19, 203–208.CrossRefGoogle Scholar