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Growth performance and immune responses in chickens after challenge with lipopolysaccharide and modulation by dietary different oils

Published online by Cambridge University Press:  01 February 2008

X. Yang ,
Y. Guo ,
X. He ,
J. Yuan ,
Y. Yang  and
Z. Wang
X. Yang
Affiliation:
The State Key Lab of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100094, People’s Republic of China College of Animal Science and Technology, Northwest A&F University, Yangling 712100, People’s Republic of China
Y. Guo*
Affiliation:
The State Key Lab of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100094, People’s Republic of China
X. He
Affiliation:
The State Key Lab of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100094, People’s Republic of China
J. Yuan
Affiliation:
The State Key Lab of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100094, People’s Republic of China
Y. Yang
Affiliation:
The State Key Lab of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100094, People’s Republic of China
Z. Wang
Affiliation:
The State Key Lab of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100094, People’s Republic of China
*
E-mail: guoym9899@yahoo.com.cn
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Abstract

The study was conducted to investigate the effects of different oils on growth performance and immune responses of chickens after challenge with lipopolysaccharide (LPS). A total of 288 chickens were assigned in a 2 × 2 factorial design. Factors were dietary fat type (4.5% maize oil or 4.5% fish oil) and immunological challenge (LPS or saline). At 20 days and 27 days of age, chickens were injected intraperitoneally with either 1 mg/kg body weight of LPS or sterile saline. LPS decreased feed intake from 21 days to 28 days of age and body-weight gain from 21 days to 42 days of age. Fish oil improved feed-conversion efficiency of chickens after LPS challenge for the first time. Fish oil supplementation decreased lymphocyte proliferation (21 days: P < 0.0001; 28 days: P < 0.0001) and the ratio of CD3+CD4+/CD3+CD8+ (21 days: P = 0.0479; 28 days: P = 0.0009) after LPS challenge. LPS challenge increased the levels of interleukin-1 (IL-1) (21 days: P < 0.0001; 28 days: P = 0.0030), IL-6 (21 days: P < 0.0001; 28 days: P = 0.0001) and tumor necrosis factor-α (TNF-α) (21 days: P = 0.0008; 28 days: P = 0.0018). And fish oil alleviated the elevations in the production of IL-6 (21 days: P = 0.0359; 28 days: P = 0.0302) and TNF-α (21 days: P = 0.0055; 28 days: P = 0.0391) induced by the LPS challenge. Fish oil alleviated the mRNA abundance elevation of nuclear factor kappa B (NFκB) (21 days: P = 0.0079; 28 days: P = 0.0017) after LPS challenge. These results showed that fish oil acts as an anti-inflammatory agent, which may be associated with down-regulation of the activated immune system. The results of pro-inflammatory cytokines and mRNA abundance results suggested that fish oil might alleviate the elevation of IL-6 and TNF-α induced by LPS through down-regulating NFκB expression.

Keywords

chickensfish oilimmune responsesmaize oilpro-inflammatory cytokines
Type
Full Paper
Information
animal , Volume 2 , Issue 2 , February 2008 , pp. 216 - 223
Copyright
Copyright © The Animal Consortium 2008

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