Hostname: page-component-76fb5796d-22dnz Total loading time: 0 Render date: 2024-04-27T02:22:34.187Z Has data issue: false hasContentIssue false
  • English
  • Français

Feed efficiency and the liver proteome of fattening lambs are modified by feed restriction during the suckling period

Published online by Cambridge University Press:  24 January 2018

A. Santos ,
C. Valdés ,
F. J. Giráldez ,
S. López ,
J. France ,
J. Frutos ,
M. Fernández  and
S. Andrés
A. Santos
Affiliation:
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas, E-24346, Grulleros, León, Spain
C. Valdés
Affiliation:
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas, E-24346, Grulleros, León, Spain
F. J. Giráldez
Affiliation:
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas, E-24346, Grulleros, León, Spain
S. López
Affiliation:
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas, E-24346, Grulleros, León, Spain
J. France
Affiliation:
Department of Animal Biosciences, Centre for Nutrition Modelling, University of Guelph, Guelph, ON, Canada, N1G 2W1
J. Frutos
Affiliation:
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas, E-24346, Grulleros, León, Spain
M. Fernández
Affiliation:
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas, E-24346, Grulleros, León, Spain
S. Andrés*
Affiliation:
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Finca Marzanas, E-24346, Grulleros, León, Spain
*
†E-mail: sonia.andres@eae.csic.es
Get access

Abstract

The present study was designed to describe the effects of early feed restriction of Merino lambs on feed efficiency during the fattening period by examining ruminal microbiota and fermentation parameters, gastrointestinal morphology, digestibility or liver proteome. In total, 24 male Merino lambs were randomly assigned to two experimental treatments (n=12 per treatment). Lambs of the first group (ad libitum (ADL)) were kept permanently with the dams, whereas the other 12 lambs (restricted (RES)) were milk restricted. When lambs reached a live BW (LBW) of 15 kg, all the animals were offered the same complete pelleted diet (35 g dry matter/kg LBW per day) until slaughter at a LBW of 27 kg. The RES lambs showed poorer feed efficiency during the fattening period when compared with the ADL group (feed to gain ratio, 3.69 v. 3.05, P<0.001). No differences were observed in ruminal microbiota, fermentation parameters or apparent digestibility. However, the proportion of the small intestine and the length of ileal villi were reduced in the RES lambs. In total, 26 spots/proteins were identified in the liver proteomic profile, with significant differences (P<0.05) between experimental treatments, suggesting a higher catabolism of proteins and a reduction in β-oxidation of fatty acids in RES lambs when compared with the ADL animals. In conclusion, early feed restriction of Merino lambs during the suckling period promotes long-term effects on the small intestine and the proteomic profile of the liver, which may influence the metabolic use of nutrients, thus negatively affecting feed efficiency during the fattening phase.

Keywords

feed restrictionfeed efficiencymetabolic programminglambliver proteome
Type
Research Article
Information
animal , Volume 12 , Issue 9 , September 2018 , pp. 1838 - 1846
Copyright
© The Animal Consortium 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alexandre, PA, Kogelman, LJA, Santana, MHA, Passarelli, D, Pulz, LH, Fantinato-Neto, P, Silva, PL, Leme, PR, Strefezzi, RF, Coutinho, LL, Ferraz, JBS, Eler, JP, Kadarmideen, HJ and Fukumasu, H 2015. Liver transcriptomic networks reveal main biological processes associated with feed efficiency in beef cattle. BMC Genomics 16, 1073.Google Scholar
Andrés, S, Bodas, R, Tejido, ML, Giráldez, FJ, Valdés, C and López, S 2016. Effects of the inclusion of flaxseed and quercitin on the diet of fattening lambs on ruminal microbiota, in vitro fermentation and biohydrogenation of fatty acids. Journal of Agricultural Science 154, 542–552.Google Scholar
AOAC 2003. Official methods of analysis, 17th edition. Association of Analytical Chemists, Gaithersburg, MD, USA.Google Scholar
Babady, NE, Pang, YP, Elpeleg, O and Isaya, G 2007. Cryptic proteolytic activity of dihydrolipoamide dehydrogenase. Proceedings of the National Academy of Sciences of the United States of America 104, 6158–6163.Google Scholar
Bell, AW and Greenwood, PL 2016. Prenatal origin of postnatal variation in growth, development and productivity in ruminants. Animal Production Science 56, 1217–1232.Google Scholar
Bhatt, RS, Tripathi, MK, Verna, DL and Karim, SA 2009. Effect of different feeding regimes on pre-weaning growth, rumen fermentation and its influence on post-weaning performance of lambs. Journal of Animal Physiology and Animal Nutrition 93, 568–573.Google Scholar
Boison, D, Scheurer, L, Zumsteg, V, Rülicke, T, Litynski, P, Fowler, B, Brandner, S and Mohler, H 2002. Neonatal hepatic steatosis by disruption of the adenosine kinase gene. Proceedings of the National Academy of Sciences of the United States of America 99, 6985–6990.Google Scholar
Carro, MD and Miller, EL 1999. Effect of supplementing a fibre basal diet with different nitrogen forms on ruminal fermentation and microbial growth in an in vitro semi-continuous culture system (RUSITEC). British Journal of Nutrition 82, 149–157.Google Scholar
Chappell, VL, Thompson, MD, Jeschke, MG, Chung, DH, Thompson, JC and Wolf, SE 2003. Effects of incremental starvation on gut mucosa. Digestive Diseases and Sciences 48, 765–769.Google Scholar
Chen, XJ, Wang, XW, Kaufman, BA and Butow, RA 2005. Aconitase couples metabolic regulation to mitochondrial DNA maintenance. Science 307, 714–717.Google Scholar
Chen, Y, Gondro, C, Quinn, K, Herd, RM, Parnell, PF and Vanselow, B 2011. Global gene expression profiling reveals genes expressed differentially in cattle with high and low residual feed intake. Animal Genetics 42, 475–490.Google Scholar
Davies, DAR and Owen, JB 1967. The intensive rearing of lambs 1. Some factors affecting performance in the liquid feeding period. Animal Science 9, 501–508.Google Scholar
Denman, SE, Tomkins, NW and McSweeney, CS 2007. Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane. FEMS Microbiology Ecology 62, 313–322.Google Scholar
de Paula, EFE, de Souza, DF, Monteiro, ALG, Santana, MHA, Gilaverte, S, Rossi, P Jr and Dittrich, RL 2013. Residual feed intake and hematological and metabolic blood profiles of lle de France lambs. Revista Brasileira de Zootecnia – Brazilian Journal of Animal Science 42, 806–812.Google Scholar
Fu, L, Xu, Y, Hou, Y, Qi, X, Zhou, L, Liu, H, Luan, Y, Jing, L, Miao, Y, Zhao, S, Liu, H and Li, X 2017. Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs. Scientific Reports 7, 45291.Google Scholar
Galvani, DB, Pires, CC, Hübner, CH, Carbalho, S and Wommer, TP 2014. Growth performance and carcass traits of early-weaned lambs as affected by the nutritional regimen of lactating ewes. Small Ruminant Research 120, 1–5.Google Scholar
Gotoh, T 2015. Potential of the application of epigenetics in animal production. Animal Production Science 55, 145–158.Google Scholar
Greenwood, PL, Hunt, AS and Bell, AW 2004. Effects of birth weight and postnatal nutrition on neonatal sheep: IV. Organ growth. Journal of Animal Science 82, 422–428.Google Scholar
Grubbs, JK, Fritchen, AN, Huff-Lonergan, E, Gabler, NK and Lonergan, SM 2013. Selection for residual feed intake alters the mitochondria protein profile in pigs. Journal of Proteomics 80, 334–345.Google Scholar
Harvey, PA and Leinwand, LA 2011. The cell biology of disease cellular mechanisms of cardiomyopathy. The Journal of Cell Biology 194, 355–365.Google Scholar
Makovicky, P, Tumova, E, Volek, Z, Makovicky, P and Vodicka, P 2014. Histological aspects of the small intestine under variable feed restriction: the effects of short and intense restriction on a growing rabbit model. Experimental and Therapeutic Medicine 8, 1623–1627.Google Scholar
Meyer, AM, Caton, JS, Hess, BW, Ford, SP and Reynolds, LP 2012. Epigenetics and effects on the neonate that may impact feed efficiency. In Feed efficiency in the beef industry (ed. RA Hill), pp. 199–223. John Willey & Sons. Inc, Ames, IA, USA.Google Scholar
Meyers, DE, Basha, HI and Koenig, MK 2013. Mitochondrial cardiomyopathy, pathophysiology, diagnosis, and management. Texas Heart Institute Journal 40, 385–394.Google Scholar
Ouwerkerk, D, Klieve, AV and Forster, RJ 2002. Enumeration of Megasphaera elsdenii in rumen contents by real-time Taq nuclease assay. Journal of Applied Microbiology 92, 753–758.Google Scholar
Quijano, C, Trujillo, M, Castro, L and Trostchansky, A 2016. Interplay between oxidant species and energy metabolism. Redox Biology 8, 28–42.Google Scholar
Rui, L 2014. Energy Metabolism in the Liver. Comprehensive. Physiology 4, 177–197.Google Scholar
Santos, A, Giráldez, FJ, Groenen, MAM, Madsen, O, Frutos, J, Valdés, C and Andrés, S 2017. Modifications in liver transcriptomic profile of fattening lambs by early suckled milk intake level. In The 68th Annual Meeting of the European Federation of Animal Science (EAAP), Tallinn, Estonia. (Theater presentation 43: 390).Google Scholar
Santos, A, Giráldez, FJ, Mateo, J, Frutos, J and Andrés, S 2018. Programming Merino lambs by early feed restriction reduces growth rates and increases fat accretion during the fattening period with no effect on meat quality traits. Meat Science 135, 20–26.Google Scholar
Stevenson, DM and Weimer, PJ 2007. Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR. Applied Microbiology and Biotechnology 75, 165–174.Google Scholar
Tizioto, PC, Coutinho, LL, Decker, JE, Schnabel, RD, Rosa, KO, Oliveira, PSN, Souza, MM, Mourão, GB, Tullio, RR, Chaves, AS, Lanna, DPD, Zerlotini-Neto, A, Mudadu, MA, Taylor, JF and Regitano, LCA 2015. Global liver gene expression differences in Nelore steers with divergent residual feed intake phenotypes. BMC Genomics 16, 242.Google Scholar
Van Keulen, JV and Young, BA 1977. Evaluation of acid insoluble ash as a natural marker in ruminant digestibility studies. Journal of Animal Science 44, 282–287.Google Scholar
Vincent, A, Louveau, I, Gondret, F, Tréfeu, C, Gilbert, H and Lefaucheur, L 2015. Divergent selection for residual feed intake affects the transcriptomic and proteomic profiles of pig skeletal muscle. Journal of Animal Science 93, 2745–2758.Google Scholar
Wai, T, García-Prieto, J, Baker, MJ, Merkwirth, C, Benit, P, Rustin, P, Rupérez, FJ, Barbas, C, Ibañez, B and Langer, T 2015. Imbalanced OPA1 processing and mitochondrial fragmentation cause heart failure in mice. Science 350, 6265.Google Scholar
Weimer, PJ. 2015. Redundancy, resilience, and host specificity of the ruminal microbiota: implications for engineering improved ruminal fermentation. Frontiers in Microbiology 6, 296.Google Scholar
Yáñez-Ruiz, DR, Abecia, L and Newbold, CJ 2015. Manipulating microbioma and fermentation through interventions during early life: a review. Frontiers in Microbiology 6, 1133.Google Scholar