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Effects of methionine hydroxy analogue supplementation on the expression of antioxidant-related genes of acute heat stress-exposed broilers

Published online by Cambridge University Press:  12 October 2017

E. Gasparino ,
A. P. Del Vesco ,
A. S. Khatlab ,
V. Zancanela ,
D. O. Grieser  and
S. C. C. Silva
E. Gasparino*
Affiliation:
Department of Animal Science, State University of Maringá, Avenida Colombo, 5790, Maringá, Paraná, Brazil
A. P. Del Vesco
Affiliation:
Department of Animal Science, State University of Maringá, Avenida Colombo, 5790, Maringá, Paraná, Brazil
A. S. Khatlab
Affiliation:
Department of Animal Science, State University of Maringá, Avenida Colombo, 5790, Maringá, Paraná, Brazil
V. Zancanela
Affiliation:
Department of Animal Science, State University of Maringá, Avenida Colombo, 5790, Maringá, Paraná, Brazil
D. O. Grieser
Affiliation:
Department of Animal Science, State University of Maringá, Avenida Colombo, 5790, Maringá, Paraná, Brazil
S. C. C. Silva
Affiliation:
Department of Animal Science, State University of Maringá, Avenida Colombo, 5790, Maringá, Paraná, Brazil
*
†E-mail: egasparino@uem.br
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Abstract

We evaluated the effects of heat stress (HS) and methionine supplementation on biological markers of stress and expression of the genes for superoxide dismutase (SOD), thioredoxin (TRx), thioredoxin reductase 1 (TRxR1) and methionine sulfoxide reductase A (MsrA) in broilers aged 1 to 21 or 22 to 42 days. The broilers were divided into two treatments, one with the recommended level of methionine supplementation (MS, supplementation of dl-2-hydroxy-4-methylthiobutanoic acid (dl-HMTBA)) and one without methionine supplementation (MD). The animals were maintained at a temperature of thermal comfort or one of HS (38°C for 24 h). Mortality was only observed in 42-day-old broilers exposed to HS and fed the MD diet, and the rate was 5%. Starter period: we observed an interaction effect between diet and temperature on the gene expression of TRxR1 and MsrA, and expression of these genes was higher in the HS animals that received the MS diet than that in birds with the MD diet. Grower period: the expression of SOD, TRxR1 and MsrA genes, activities of aspartate aminotransferase (AST) and creatine kinase (CK) and content of creatinine were influenced by both study variables. In the HS animals, the expression of these genes, AST activity and creatinine content increased and CK activity decreased. In the animals on the MD diet, the expression of these genes and AST and creatinine values were higher and the CK activity was lower than those for the birds on the MS diet. Our results indicated that under HS conditions, the supplementation with dl-HMTBA could mitigate major damage caused by stress through the action on some genes related to TRx complex activity.

Keywords

antioxidantbroilerheat stressoxidative stressthioredoxin
Type
Research Article
Information
animal , Volume 12 , Issue 5 , May 2018 , pp. 931 - 939
Copyright
© The Animal Consortium 2017 

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Footnotes

a

Present address: Animal Science Department, Federal University of Sergipe, Aracaju, Sergipe, Brazil.

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