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Dietary protein, energy and arginine affect LAT1 expression in forebrain white matter differently

Published online by Cambridge University Press:  06 April 2010

X. Wu ,
Y. L. Yin ,
T. J. Li ,
L. Wang ,
Z. Ruan ,
Z. Q. Liu  and
Y. Q. Hou
X. Wu
Affiliation:
State Key Laboratory of Food Science and Technology and College of Life Science and Food Engineering, Nanchang University, Nanchang 330047, China Hunan Engineering and Research Center of Animal and Poultry Science and Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, China
Y. L. Yin*
Affiliation:
State Key Laboratory of Food Science and Technology and College of Life Science and Food Engineering, Nanchang University, Nanchang 330047, China Hunan Engineering and Research Center of Animal and Poultry Science and Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, China Hubei Feedstuff Key Laboratory of Wuhan Polytechnic University, Wuhan, China
T. J. Li
Affiliation:
Hunan Engineering and Research Center of Animal and Poultry Science and Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, China
L. Wang
Affiliation:
Hubei Feedstuff Key Laboratory of Wuhan Polytechnic University, Wuhan, China
Z. Ruan
Affiliation:
State Key Laboratory of Food Science and Technology and College of Life Science and Food Engineering, Nanchang University, Nanchang 330047, China
Z. Q. Liu
Affiliation:
State Key Laboratory of Food Science and Technology and College of Life Science and Food Engineering, Nanchang University, Nanchang 330047, China
Y. Q. Hou
Affiliation:
Hubei Feedstuff Key Laboratory of Wuhan Polytechnic University, Wuhan, China
*
†E-mail: yyulong2003@yahoo.com.cn
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Abstract

L-type amino acid transporter-1 (LAT1) transports large, branched-chain, aromatic and neutral amino acids. About 64 Duroc × Landrace × Yorkshire pigs were used to study the effects of dietary crude protein (CP), energy and arginine on LAT1 expression in forebrain. The results showed that LAT1 expression in forebrain was sensitive to different levels of CP, energy and arginine. On the basis of Western blot analysis, a lower level of LAT1 presented in the brain tissues of pigs fed the low dietary CP diet (P < 0.05), a higher level were found in pigs fed the higher CP diet, with moderate to intense staining seen in pigs fed the diet plus 1% arginine. In contrast, pigs fed the control-energy diet had weak LAT1 expression, and those fed the diet supplemented with 1% arginine showed lowest LAT1 expression (P < 0.05). These results showed that LAT1 was highly expressed in the forebrain, and expression of LAT1 was affected by dietary protein, energy and arginine differently.

Keywords

L-type amino acid transporter 1dietary proteinenergyargininebrain
Type
Full Paper
Information
animal , Volume 4 , Issue 9 , September 2010 , pp. 1518 - 1521
Copyright
Copyright © The Animal Consortium 2010

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