Hostname: page-component-76fb5796d-25wd4 Total loading time: 0 Render date: 2024-04-26T03:24:20.365Z Has data issue: false hasContentIssue false
  • English
  • Français

Dietary L-arginine supplementation reduces abdominal fat content by modulating lipid metabolism in broiler chickens

Published online by Cambridge University Press:  11 March 2013

A. M. Fouad ,
H. K. El-Senousey ,
X. J. Yang  and
J. H. Yao
A. M. Fouad
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China Department of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
H. K. El-Senousey
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China Department of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
X. J. Yang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
J. H. Yao*
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
*
†E-mail: yaojunhu2004@sohu.com
Get access

Abstract

This study investigated the effects of different levels of dietary L-arginine (L-Arg) supplementation on the abdominal fat pad, circulating lipids, hepatic fatty acid synthase (FAS) gene expression, gene expression related to fatty acid β-oxidation, and the performance of broiler chickens. We tested whether the dietary L-Arg levels affected the expression of genes related to lipid metabolism in order to reduce body fat deposition. A total of 192 broiler chickens (Cobb 500) aged 21 days with an average BW of 920 ± 15 g were randomly assigned to four groups (six broilers per replicate and eight replicates per treatment). The control group was fed a basal diet, whereas the treatment groups were fed basal diets supplemented with 0.25%, 0.50%, or 1.00% L-Arg for 3 weeks. The average daily feed intake, average daily gain and feed : gain ratio were not affected by the dietary L-Arg levels. However, chickens supplemented with L-Arg had lower abdominal fat content, plasma triglyceride (TG), total cholesterol (TC) concentrations, hepatic FAS mRNA expression and increased heart carnitine palmitoyl transferase1 (CPT1) and 3-hydroxyacyl-CoA dehydrogenase (3HADH) mRNA expression. These findings suggest that the addition of 0.25% L-Arg may reduce the plasma TC concentration by decreasing hepatic 3-hydroxyl-3-methylglutaryl-CoA reductase mRNA expression. This may lower the plasma TG and abdominal fat content by suppressing hepatic FAS mRNA expression and enhancing CPT1 and 3HADH (genes related to fatty acid β-oxidation) mRNA expression in the hearts of broiler chickens.

Keywords

L-argininechickensfatty acid β-oxidationfatty acid synthase
Type
Nutrition
Information
animal , Volume 7 , Issue 8 , August 2013 , pp. 1239 - 1245
Copyright
Copyright © The Animal Consortium 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Atakisi, O, Atakisi, E, Kart, A 2009. Effects of dietary zinc and L-arginine supplementation on total antioxidants capacity, lipid peroxidation, nitric oxide, egg weight, and blood biochemical values in Japanese quails. Biological Trace Element Research 132, 136–143.Google Scholar
Ball, RO, Urschel, KL, Pencharz, PB 2007. Nutritional consequences of interspecies differences in arginine and lysine metabolism. Journal of Nutrition 137, 1626–1641.Google Scholar
Butterwith, SC 1989. Contribution of lipoprotein lipase activity to the differential growth of three adipose tissue depots in young broiler chickens. British Poultry Science 30, 927–933.Google Scholar
Chen, J, Wang, M, Kong, Y, Ma, H, Zou, S 2011. Comparison of the novel compounds creatine and pyruvate on lipid and protein metabolism in broiler chickens. Animal 5, 1082–1089.Google Scholar
Choct, M, Naylor, A, Hutton, O, Nolan, J 2000. Increasing efficiency of lean tissue composition in broiler chickens. A Report for the Rural Industries Research and Development Corporation. Publication No 98/123. Retrieved August 20, 2010, from https://rirdc.infoservices.com.au/downloads/98-123 .Google Scholar
Clemmensen, C, Madsen, AN, Smajilovic, S, Holst, B, Brauner-Osborne, H 2012. L-Arginine improves multiple physiological parameters in mice exposed to diet-induced metabolic disturbances. Amino Acids 43, 1265–1275.Google Scholar
Corzo, A, Moran, ET Jr, and Hoehler, D 2003. Arginine need of heavy broiler males: applying the ideal protein concept. Poultry Science 82, 402–407.Google Scholar
Cui, HX, Zheng, MQ, Liu, RR, Zhao, GP, Chen, JL, Wen, J 2012. Liver dominant expression of fatty acid synthase (FAS) gene in two chicken breeds during intramuscular-fat development. Molecular Biology Reports 39, 3479–3484.Google Scholar
Cui, HX, Yang, SY, Wang, HY, Zhao, JP, Jiang, RR, Zhao, GP, Chen, JL, Zheng, MQ, Li, XH, Wen, J 2010. The effect of a mutation in the 3-UTR region of the HMGCR gene on cholesterol in Beijing-you chickens. Animal Biotechnology 21, 241–251.Google Scholar
D'Amato, JL, Humphrey, BD 2010. Dietary arginine levels alter markers of arginine utilization in peripheral blood mononuclear cells and thymocytes in young broiler chicks. Poultry Science 89, 938–947.Google Scholar
Eaton, S 2002. Control of mitochondrial beta-oxidation flux. Progress in Lipid Research 41, 197–239.Google Scholar
El-Kirsh, AA, Abd El-Wahab, HM, Abd-Ellah Sayed, HF 2011. The effect of L-arginine or L-citrulline supplementation on biochemical parameters and the vascular aortic wall in high-fat and high-cholesterol-fed rats. Cell Biochemistry & Function 29, 414–428.Google Scholar
Emadi, M, Jahanshiri, F, Kaveh, K, Hair-Bejo, M, Ideris, A, Alimon, AR 2011. Nutrition and immunity: the effects of the combination of arginine and tryptophan on growth performance, serum parameters and immune response in broiler chickens challenged with infectious bursal disease vaccine. Avian Pathology 40, 63–72.Google Scholar
Emmerson, DA 1997. Commercial approaches to genetic selection for growth and feed conversion in domestic poultry. Poultry Science 76, 1121–1125.Google Scholar
Fernandes, JI, Murakami, AE, Martins, EN, Sakamoto, MI, Garcia, ER 2009. Effect of arginine on the development of the pectoralis muscle and the diameter and the protein:deoxyribonucleic acid rate of its skeletal myofibers in broilers. Poultry Science 88, 1399–1406.Google Scholar
Havenstein, GB, Ferket, PR, Qureshi, MA 2003. Carcass composition and yield of 1957 versus 2001 broilers when fed representative 1957 and 2001 broiler diets. Poultry Science 82, 1509–1518.Google Scholar
Jobgen, W, Meininger, CJ, Jobgen, SC, Li, P, Lee, MJ, Smith, SB, Spencer, TE, Fried, SK, Wu, G 2009. Dietary L-arginine supplementation reduces white fat gain and enhances skeletal muscle and brown fat masses in diet-induced obese rats. Journal of Nutrition 139, 230–237.Google Scholar
Jobgen, WS, Fried, SK, Fu, WJ, Meininger, CJ, Wu, G 2006. Regulatory role for the arginine-nitric oxide pathway in metabolism of energy substrates. Journal of Nutritional Biochemistry 17, 571–588.Google Scholar
Khajali, F, Wideman, RF 2010. Dietary arginine: metabolic, environmental, immunological and physiological interrelationships. World's Poultry Science Journal 66, 751–766.Google Scholar
Le Mignon, G, Pitel, F, Gilbert, H, Le Bihan-Duval, E, Vignoles, F, Demeure, O, Lagarrigue, S, Simon, J, Cogburn, LA, Aggrey, SE, Douaire, M, Le Roy, P 2009. A comprehensive analysis of QTL for abdominal fat and breast muscle weights on chicken chromosome 5 using a multivariate approach. Animal Genetic 40, 157–164.Google Scholar
Lee, JE, Austic, RE, Naqi, SA, Golemboski, KA, Dietert, RR 2002. Dietary arginine intake alters avian leukocyte population distribution during infectious bronchitis challenge. Poultry Science 81, 793–798.Google Scholar
Molette, C, Theron, L, Marty-Gasset, N, Fernandez, X, Remignon, H 2012. Current advances in proteomic analysis of (fatty) liver. Journal of Proteomics 75, 4290–4295.Google Scholar
Mujahid, A, Furuse, M 2008. Central administration of corticotropin-releasing factor induces thermogenesis by changes in mitochondrial bioenergetics in neonatal chicks. Neuroscience 155, 845–851.Google Scholar
Munir, K, Muneer, MA, Masaoud, E, Tiwari, A, Mahmud, A, Chaudhry, RM, Rashid, A 2009. Dietary arginine stimulates humoral and cell-mediated immunity in chickens vaccinated and challenged against hydropericardium syndrome virus. Poultry Science 88, 1629–1638.Google Scholar
National Research Council (NRC) 1994. Nutrient requirements of poultry, 9th edition. National Academy Press, Washington, DC, USA.Google Scholar
Pitel, F, Fillon, V, Heimel, C, Le Fur, N, el Khadir-Mounier, C, Douaire, M, Gellin, J, Vignal, A 1998. Mapping of FASN and ACACA on two chicken microchromosomes disrupts the human 17q syntenic group well conserved in mammals. Mammalian Genome 9, 297–300.Google Scholar
Santoso, U, Tanaka, K, Ohtani, S 1995. Effect of dried Bacillus subtilis culture on growth, body composition and hepatic lipogenic enzyme activity in female broiler chicks. British Journal of Nutrition 74, 523–529.Google Scholar
Skiba-Cassy, S, Collin, A, Chartrin, P, Medale, F, Simon, J, Duclos, MJ, Tesseraud, S 2007. Chicken liver and muscle carnitine palmitoyltransferase 1: nutritional regulation of messengers. Comparative Biochemistry and Physiology Part B 147, 278–287.Google Scholar
Sonaiya, EB 1985. Abdominal fat weight and thickness as predictors of total body fat in broilers. British Poultry Science 26, 453–458.Google Scholar
Tan, B, Yin, Y, Liu, Z, Li, X, Xu, H, Kong, X, Huang, R, Tang, W, Shinzato, I, Smith, SB, Wu, G 2009. Dietary L-arginine supplementation increases muscle gain and reduces body fat mass in growing-finishing pigs. Amino Acids 37, 169–175.Google Scholar
Tan, B, Yin, Y, Liu, Z, Tang, W, Xu, H, Kong, X, Li, X, Yao, K, Gu, W, Smith, SB, Wu, G 2011. Dietary L-arginine supplementation differentially regulates expression of lipid-metabolic genes in porcine adipose tissue and skeletal muscle. Journal of Nutritional Biochemistry 22, 441–445.Google Scholar
Tan, X, Sun, WD, Li, JC, Pan, JQ, Wang, XL 2006. Changes in pulmonary arteriole protein kinase c-alpha expression associated with supplemental L-arginine in broilers during cool temperature exposure. British Poultry Science 47, 230–236.Google Scholar
Tayade, C, Koti, M, Mishra, SC 2006. L-Arginine stimulates intestinal intraepithelial lymphocyte functions and immune response in chickens orally immunized with live intermediate plus strain of infectious bursal disease vaccine. Vaccine 24, 5473–5480.Google Scholar
Tzeng, R, Becker, WA 1981. Growth patterns of body and abdominal fat weights in male broiler chickens. Poultry Science 60, 1101–1106.Google Scholar
Wu, LY, Fang, YJ, Guo, XY 2011. Dietary L-arginine supplementation beneficially regulates body fat deposition of meat-type ducks. British Poultry Science 52, 221–226.Google Scholar
Zhao, GP, Chen, JL, Zheng, MQ, Wen, J, Zhang, Y 2007. Correlated responses to selection for increased intramuscular fat in a Chinese quality chicken line. Poultry Science 86, 2309–2314.Google Scholar
Supplementary material: File

Fouad Supplementary Material

Appendix

Download Fouad Supplementary Material(File)
File 34.3 KB