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Characterisation of white and black merino wools: a proteomics study

Published online by Cambridge University Press:  09 July 2018

J. Plowman ,
A. Thomas ,
T. Perloiro ,
S. Clerens  and
A. M. de Almeida Open the ORCID record for A. M. de Almeida [Opens in a new window]
J. Plowman
Affiliation:
AgResearch Ltd, 1365 Springs Road, Lincoln 7674, Private Bag Christchurch 8140, Christchurch, New Zealand
A. Thomas
Affiliation:
AgResearch Ltd, 1365 Springs Road, Lincoln 7674, Private Bag Christchurch 8140, Christchurch, New Zealand
T. Perloiro
Affiliation:
ANCORME – Associação Nacional de Criadores de Raça Ovina Merina, 7005-873 Évora, Portugal
S. Clerens
Affiliation:
AgResearch Ltd, 1365 Springs Road, Lincoln 7674, Private Bag Christchurch 8140, Christchurch, New Zealand
A. M. de Almeida
Affiliation:
Ross University School of Veterinary Medicine, PO Box 334, Basseterre, St. Kitts and Nevis, West Indies
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Abstract

Wool is an important agricultural commodity with merino wool being rated alongside the finest quality fibres, which include the goat fibres Mohair and Cashmere. Although pigmented wool merinos have become extremely rare, the market for this wool is increasing. In Portugal, there are two merino breeds: white and black, descendants of animals originally bred on the Iberian Peninsula. These breeds have the potential to assist in our understanding of how protein expression relates to wool traits of importance to the textile industry. Herein, we study the characteristics and protein expression profiles of wool from ewes of the Portuguese black and white merino (n=15). Both breeds had very similar results for fibre diameter (25 µm) and curvature (105 to 111°/mm). Significant between-breed differences were found in the two types of keratin-associated proteins (KAPs): high-sulphur proteins (HSPs) and high-glycine–tyrosine proteins (HGTPs). The expression of HSPs, KAP2-3 and KAP2-4, decreased expression in the pigmented animals, whereas KAP13-1 was found in higher amounts. Likewise, the expression of the ultra-high-sulphur proteins, KAP4-3 and KAP4-7-like, was reduced in black sheep to half the levels of the white wools, whereas the HGTPs, KAP6, KAP6-1, KAP6-2 and KAP16-2, were more abundant in black sheep. These results suggest structural differences between the black and white merino wool, because of differences among some KAPs. These differences have important implications for the textile industry.

Keywords

proteomefibre qualityhigh-sulphur proteinshigh-glycine–tyrosine proteinskeratin
Type
Research Article
Information
animal , Volume 13 , Issue 3 , March 2019 , pp. 659 - 665
Copyright
© The Animal Consortium 2018 

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Footnotes

†

Present address: Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda 1349-017 Lisboa, Portugal. E-mail: aalmeida@isa.ulisboa.pt

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