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Effect of including purified jack bean lectin in casein or hydrolysed casein-based diets on apparent and true ileal amino acid digestibility in the growing pig

Published online by Cambridge University Press:  18 August 2016

Y. -L. Yin ,
R. L. Huang ,
A. J. Libao-Mercado ,
E. A. Jeaurond ,
C. F. M. de Lange  and
M. Rademacher
Y. -L. Yin
Affiliation:
Department of Animal and Poultry Science, University ofGuelph Guelph, Ontario, NIG 2W1, Canada Key Laboratory of Subtropical Agro-ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Hunan, Changsha, PO Box 10, 410125, Peoples', Republic of China
R. L. Huang
Affiliation:
Key Laboratory of Subtropical Agro-ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Hunan, Changsha, PO Box 10, 410125, Peoples', Republic of China
A. J. Libao-Mercado
Affiliation:
Department of Animal and Poultry Science, University ofGuelph Guelph, Ontario, NIG 2W1, Canada
E. A. Jeaurond
Affiliation:
Department of Animal and Poultry Science, University ofGuelph Guelph, Ontario, NIG 2W1, Canada
C. F. M. de Lange*
Affiliation:
Department of Animal and Poultry Science, University ofGuelph Guelph, Ontario, NIG 2W1, Canada
M. Rademacher
Affiliation:
Degussa AG, PO Box 1345, D-63403, Hanau, Germany
*
†Corresponding author E-mail: cdelange@uoguelph.c
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Abstract

The effect of including purified jack bean lectin (concanavalin A) in the diet of grower pigs on endogenous ileal nitrogen (N) and amino acid (AA) losses, and apparent and true ileal AA digestibility was investigated in a study with four growing pigs (average initial live weight (LW) 25 kg) fitted with simple T-cannulae at the terminal ileum. Both the homo-arginine (HA) method and peptide-alimentation method were used to estimate endogenous gut AA losses. The animals were offered four diets :(1) an enzymatically hydrolysed casein (EHC) containing diet, (2) an EHC containing diet supplemented with 266 mg/kg jack bean lectin, (3) a normal casein (NC) containing diet, and (4) a NC containing diet supplemented with 266 mg/kg lectin. For the estimation of ileal endogenous AA flows in pigs given NC diets, a subsample ofNC was guanidinated to convert lysine to HA. The experiment was conducted using a 4X4 Latin-square design. In each experimental period, pigs were adjusted to the experimental diets for 5 days. On day 6, ileal digesta was collected continuously for 24 hfor determination of apparent ileal digestibility. At 08:30 h of day 8 the HA NC diets were offered to pigs on NC treatments and ileal digesta were again collected for 24 h from all pigs. There were no interactive effects of casein type and added dietary lectin on apparent and true ileal AA digestibility and endogenous ileal AA flows (P > 0-05). The addition of lectin to the diet did not influence (P > 0-05) the apparent ileal digestibility of dry matter (DM), crude protein (CP) and AA. Casein type did not affect ileal DM digestibility (P > 0-05). Pigs given the NC-based diets had higher (P < 0-05) apparent ileal AA digestibility than pigs given the EHC-based diets. Addition of lectin to the diet did not influence (P > 0-05) endogenous ileal CP and AA flows. Endogenous ileal AA losses were generally higher (P < 0-05) in pigs given EHC diets than in pigs given NC diets. Neither casein type nor diet lectin level influenced (P > 0-05) the true ileal digestibility ofCP and AA; values ranged for most AA between 0-98 and 1-00. These data suggest that including purified jack bean lectin at the level of 266 mg/kg in a casein-based diet did not influence the ileal endogenous CP and AAflow, nor the apparent and the true ileal digestibility ofCP and AA, in growing pigs between 25 and 50 kg LW. Compared with the HA method, the peptide-alimentation method yielded higher estimates of ileal endogenous CP and AA acid losses in pigs given casein-based diets, although these two methods yielded similar estimates of true ileal AA and CP digestibility.

Keywords

amino acidsdigestibilityileumlectinspigs
Type
Non-ruminant nutrition, behaviour and production
Information
Animal Science , Volume 79 , Issue 2 , October 2004 , pp. 283 - 291
Copyright
Copyright © British Society of Animal Science 2004

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References

Association of Official Analytical Chemists. 1990. Official methods of analysis, 16th edition. AOAC, Washington, DC.Google Scholar
Centraal Veevoeder Bureau. 1998. Nutritive value of feed ingredients. Centraal Veevoeder Bureau, Postbus 2176, 8203 AD Lelystad, The Netherlands.Google Scholar
Darcy-Vrillon, B., Souffrant, W. B., Laplace, J. P., Reret, A., Corring, X, Vaugelade, P., Gebhardt, G. and Kohler, R. 1991. Exogenous and endogenous contributions to nitrogen fluxes in the digestive tract of pigs fed casein diet. II. Ileal and faecal digestibility and absorption of amino acids. Reproduction, Nutrition, Development 31: 561–573.CrossRefGoogle ScholarPubMed
de Lange, C. F. M., Nyachoti, C. M. and Verstegen, M. W. A. 2000. The significance of anti-nutritional factors in feedstuffs for monogastric animals. In Feed evaluation: principles and practice (ed. Moughan, P. J., Verstegen, M. W. A. and Visser-Reyneveld, M. I.), pp. 169–183. Wageningen Pers, Wageningen, The Netherlands.Google Scholar
de Lange, C. F. M., Sauer, W. C, Mosenthin, R. and Souffrant, W. B. 1989. The effect of feeding different protein-free diets on the recovery and amino acid composition of endogenous protein collected from the distal ileum and feces in pigs. Journal of Animal Science 67: 746–754.Google Scholar
de Lange, C. F. M., Sauer, W. C, Mosenthin, R. and Souffrant, W. B. and Lien, K. A. 1992. 15N-leucine and 15N-isoleucine isotope dilution techniques versus the 15Nisotope dilution technique for determining the recovery of endogenous protein and amino acids in digesta collected from the distal ileum in pigs. Journal of Animal Science 70: 1848–1856.Google Scholar
Grant, G. and Driessche, E. van. 1993. Legume lectins: physico-chemical and nutritional properties. In Recent advances of research in antinutritional factors in legume seeds (ed. van der Poel, A. F. B. and Huisman, J.), pp. 219–231. Pudoc, Wageningen, The Netherlands.Google Scholar
Hagenmeister, H. and Erbersdobler, H. 1985. Chemical labelling of dietary protein by transformation of lysine to homoarginine: a new technique to follow intestinal digestion and absorption. Proceedings of the Nutrition Society 44:133A.Google Scholar
Hansen, L. and Hoist, J. J. 2002. The effects of duodenal peptides on glucagon-like peptide-1 secretion from the ileum. Regulation of Peptide 110:39–45.Google Scholar
Hodgkinson, S. M. and Moughan, P. J. 2000. Amino acids: digestibility, availability and metabolism. In Peed evaluation: principles and practice (ed. Moughan, P. J., Verstegen, M. W. A., and Visser-Reyneveld, M. I.), pp. 125–131. Wageningen Press, The Netherlands.Google Scholar
Hodgkinson, S. M., Moughan, P. J., Reynolds, G. W. and James, K. A. 2000. The effect of dietary peptide concentration on endogenous ileal amino acid loss in the growing pig. British Journal of Nutrition 83:421–430.Google Scholar
Huisman, J. A., van der Poel, F. B., Mouwen, J. M. V. M. and van Weerden, E. J. 1990. Effect of variable protein contents in diets containing Phaseolus vulgaris beans on performance, organ weights and blood variables in piglets, rats and chickens. British Journal of Nutrition 64: 755–764.Google Scholar
Jansman, A. J. M., Smink, W., Leeuwen, P. van and Rademacher, R. R. 2002. Evaluation through literature data of the amount and amino acid composition of basal endogenous crude protein at the terminal ileum of pigs. Animal Peed Science and Technology 98:49–60.Google Scholar
Jayne-Williams, D. J. 1973. Influence of dietary jack beans (Canavalia ensiformis) and of Concanavalin A on the growth of conventional and gnotobiotic Japanese quail (Coturnix coturnix japonica). Nature New Biology 243:150–151.Google Scholar
King, T. P., Begbie, R. and Cadenhead, A. 1983. Nutritional toxicity of raw kidney beans in pigs. Immunocytochemical and cytopathological studies on the gut and the pancreas. Journal of the Science of Food and Agriculture 34:1404–1412.Google Scholar
Llames, C. R. and Fontaine, J. 1994. Determination of amino acids in feeds: collaborative study. Journal of the Association of Official Analytical Chemists 77:1362–1366.Google Scholar
Libao, A. J. 2002. Utilization of amino acids for protein deposition in growing pigs: effect of dietary protein source. M. Sc. thesis, University of Guelph.Google Scholar
Lorenzsonn, V. and Olsen, W. A. 1982. In vivo responses of rat intestinal epithelium to intraluminal dietary lectins. Gastroenterology 82:838–848.Google Scholar
Möhn, S., Gillis, A. M., Moughan, P. J. and de Lange, C. F. M. 2000. Influence of dietary lysine and energy intakes on body protein deposition and lysine utilization in the growing pig. Journal of Animal Science 78:1510–1519.Google Scholar
Moughan, P. J. and Rutherfurd, S. M. 1990. Endogenous flow of total lysine and other amino acids at the distal ileum of the protein- or peptide-fed rat: the chemical labeling of gelatin protein by transformation of lysine to homoarginine. Journal of the Science of Food and Agriculture 52:179–192.Google Scholar
National Research Council. 1998. Nutrient requirements of swine, 10th edition. National Academy Press, Washington.Google Scholar
Nyachoti, C. M., de Lange, C. F. M., McBride, B. W. and Schulze, H. 1997a. Significance of endogenous gut nitrogen losses in the nutrition of growing pigs: a review. Canadian Journal of Animal Science. 77:149–163.CrossRefGoogle Scholar
Nyachoti, C. M., de Lange, C. F. M. and Schulze, H. 1997b. Estimating endogenous amino acid flow at the terminal ileum and true ileal amino acid digestibilities in feedstuffs for growing pigs using the homoarginine method. Journal of Animal Science 75:3206–3213.Google Scholar
Pusztai, A. 1998. Biological effects of dietary lectins. In Recent advances of research in antinutritional factors in legume seeds (ed. Huisman, J., van der Poel, T. F. B. and Liener, I.E.), pp. 17–29. Pudoc, Wageningen, The Netherlands.Google Scholar
Pusztai, A., Ewen, S. W. B., Grant, G., Peumans, W. J., Damme, E. J. M. van, Rubio, L. and Bardocz, S. 1990. Relationship between survival and binding of plant lectins during small intestinal passage and their effectiveness as growth factors. Digestion 46: (suppl. 2)308–316.Google Scholar
Pusztai, A., Ewen, S. W. B., Yule, M. and Bardocz, S. 1991. Effects of concanavalin A and Phaseolus vulgaris lecti, PAH, on the gut and systemic metabolism of germ-free and conventional rats. International workshop on Canavalia, Maracay, Venezuela, pp. 23–30.Google Scholar
Schmitz, M., Hagemeister, H. and Erbersdobler, H. F. 1991. Homoarginine labeling is suitable for determination of protein absorption in miniature pigs. Journal of Nutrition 121:1575–1580.Google Scholar
Schulze, H. 1994. Endogenous ileal nitrogen losses in pigs, dietary factors. Ph. D. thesis, Wageningen Agricultural University.Google Scholar
Seve, B. and Lahaye, L. 2003. Nitrogen and amino acid cost of digestions: dietary factors affecting ileal endogenous losses and consequences on the net availability of dietary amino acids for maintenance and growth. Proceedings of the ninth international symposium on digestive physiology in pigs, University of Alberta (ed. Ball, R. O.), p. 263.Google Scholar
Souffrant, W. B., Fevrier, C., Laplace, J. P. and Hennig, U. 1997. Comparison of methods to estimate ileal endogenous nitrogen and amino acids in piglets. In Digestive physiology in pigs (ed. Laplace, J. P., Fevrier, C., and Barbeau, A.), pp. 591–595. European Association for Animal Production publication no. 88. INRA, Saint Malo, France.Google Scholar
Souffrant, W. B., Koehler, R., Matkowitz, R., Gebhardt, G. and Schmandke, H. 1981. Ernaehrungsphysiologische Untersuchungen an Schweinen zur Beuteilung von modiifizierten Protein. Archive of Animal Nutrition 31: 675–685.Google Scholar
Statistical Analysis Systems Institute. 1988. SAS user's guide, version 6-03. SAS Institute, Inc., Cary, NC.Google Scholar
Taatjes, D. J. and Roth, J. 1991. Glycosylation in intestinal epithelium. International Review of Cytology 126:135–193.Google Scholar
Weaver, L. T. and Bailey, D. S. 1987. Effect of the lectin concanavalin A on the neonatal guinea pig gastrointestinal mucosain vivo. Journal of Pediatrics Gastroenterology Nutrition 6: 445–453.Google Scholar
Yin, Y. -L., Huang, R. -L. and Zhong, H. -Y. 1993. Effect of autoclaving on ileal digestibility of crude protein, amino acid, urease activity of beans. Zeitschrift fur Tierphysiologie, Tierernahrung und Futtermittelkunde 71: 65–74.Google Scholar