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Correlation of serum GH and IGF-1 with satellite cell responsiveness in Targhee rams

Published online by Cambridge University Press:  02 September 2010

M. V. Dodson ,
K. L. Hossner ,
J. L. Vierck ,
B. Mathison  and
E. Krabbenhoft
M. V. Dodson
Affiliation:
Muscle Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman WA 99164-6320, USA
K. L. Hossner
Affiliation:
Department of Animal Sciences, Colorado State University, Fort Collins CO 80523, USA
J. L. Vierck
Affiliation:
Muscle Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman WA 99164-6320, USA
B. Mathison
Affiliation:
Muscle Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman WA 99164-6320, USA
E. Krabbenhoft
Affiliation:
Muscle Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman WA 99164-6320, USA
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Abstract

This study was performed to assess the relationship between serum growth hormone (GH) and insulin-like growth factor 1 (IGF-1) levels, slaughter weight, and myogenic satellite cell growth kinetics of Targhee rams. Serum was collected from 19 rams at 15-min intervals over a period of 4h. Radioimmunoassays of serum samples for GH revealed considerable variation (within individual rams) over the sampling period, with mean values ranging from 0·63 to 4·88 μg/l (mean overall 2·3 (s.e. 0·33) μg/l; no. = 19). There was no significant correlation between GH levels and slaughter weight (r = −0·11; P > 0·05) at 155 (s.e. 1·08) days. Serum IGF-1 levels of (individual) rams were invariant over the sampling period, with individual means ranging from 62 to 233 μg/l (mean overall of 117 (s.e. 45·6) ugll; no. = 19). IGF-1 was not strongly correlated with slaughter weight (r = +0·35; P > 0·05). Satellite cells were isolated from the left m. semimembranosus of all rams at slaughter and grown in culture to evaluate proliferation amount and differentiation extent. The correlations between serum GH levels and satellite cell proliferation and differentiation in vitro were r = −0·53 (P < 0·05) and r = −0·52 (P < 0·05), respectively. Serum IGF-1 showed no significant correlations to proliferation (r = +0·07; P > 0·05) or to differentiation (r = −0·07; P > 0·05) of the satellite cells. These data suggest that serum GH levels in Targhee rams may not reflect muscle growth potential if correlated to body weight of 155 days. Furthermore, as IGF-1 was not correlated significantly with slaughter weight or to variables of satellite cell proliferation and differentiation, another mode of satellite cell regulation (possibly paracrine controllers) is more likely at play to coordinate the satellite cell involvement in muscle growth in Targhee rams at 155 days.

Keywords

differentiationproliferationsatellite cellssheepsomatomedinsomatotropin
Type
Research Article
Information
Animal Science , Volume 62 , Issue 1 , February 1996 , pp. 89 - 96
Copyright
Copyright © British Society of Animal Science 1996

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