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Human intestinal spirochetosis diagnosed with colonoscopy and analysis of partial 16S rDNA sequences of involved spirochetes

Published online by Cambridge University Press:  28 February 2007

Wolfgang Kraatz ,
Ulf Thunberg ,
Bertil Pettersson  and
Claes Fellström
Wolfgang Kraatz
Affiliation:
Department of Pathology, The University Hospital, Uppsala, Sweden
Ulf Thunberg
Affiliation:
Department of Oncology, The University Hospital, UppsalaSweden
Bertil Pettersson
Affiliation:
Department of Biotechnology, The Royal Institute of Technology, StockholmSweden
Claes Fellström*
Affiliation:
Department of Medicine and Surgery, Swedish University of Agricultural Sciences, Faculty of Veterinary Medicine, Uppsala, Sweden
*
*Department of Large Animal Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Box 7018, SE-750 07 Uppsala, Sweden. E-mail: Claes.Fellstrom@kirmed.slu.se
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Abstract

DNA was extracted from colonic biopsies of 33 patients with and three without evidence of intestinal spirochetosis (IS) in the large bowel. The biopsies were subjected to PCR. A pair of primers, generating a 207 bp fragment, were designed to detect specifically the 16S rDNA gene of Brachyspira. PCR products of the expected size were obtained from 33 samples with histologic evidence of IS. The PCR amplicons were used for sequencing. The sequences obtained were aligned to the corresponding 16S rRNA sequences of five type strains of Brachyspira. The sequences of 23 PCR products were 99–100% identical with the correspond-ing B. aalborgi type strain sequence. Two cases showed 99–100% sequence similarity with the type strain of B. pilosicoli P43/6/78. Six cases could not be referred to any of the known species of Brachyspira. Two PCR products gave incomplete sequences.

Type
Research Article
Information
Animal Health Research Reviews , Volume 2 , Issue 1 , June 2001 , pp. 111 - 116
Copyright
Copyright © CAB International 2001

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