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Caracterización genética de seis proteínas lácteas en tres razas bovinas cubanas

Published online by Cambridge University Press:  01 August 2011

O. Uffo ,
I. Martín-Burriel ,
S. Martínez ,
R. Ronda ,
R. Osta ,
C. Rodellar  and
P. Zaragoza
O. Uffo
Affiliation:
Centro Nacional de Sanidad Agropecuaria (CENSA), San José de las Lajas, apartado 10, CP 32 700, La Habana, Cuba
I. Martín-Burriel
Affiliation:
Laboratorio de Genética Bioquímica, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013, Zaragoza, España
S. Martínez
Affiliation:
Centro Nacional de Sanidad Agropecuaria (CENSA), San José de las Lajas, apartado 10, CP 32 700, La Habana, Cuba
R. Ronda
Affiliation:
Laboratorio de Genética Bioquímica, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013, Zaragoza, España
R. Osta
Affiliation:
Laboratorio de Genética Bioquímica, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013, Zaragoza, España
C. Rodellar
Affiliation:
Laboratorio de Genética Bioquímica, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013, Zaragoza, España
P. Zaragoza
Affiliation:
Laboratorio de Genética Bioquímica, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013, Zaragoza, España
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Resumen

En el presente estudio se presentan los primeros resultados de la determinación de la estructura genética de tres poblaciones de ganado bovino autóctono cubano: Criollo de Cuba, Cebú Cubano y Siboney de Cuba, para los loci de seis proteínas lácteas (CASA1, CASAB, CASA2, CASK, LAA y LGB). Se analizaron un total de 150 individuos (50 por cada raza), mediante análisis de ADN por PCR-RFLP.Se calcularon las frecuencias alélicas para cada locus así como condiciones de equilibrio de Hardy-Weinberg. Fueron identificados los alelos CASA1C y LAAA como evidencia de la presencia de genes de Bos indicus en las tres poblaciones cubanas. Se comprobó la existencia de elevada variabilidad en cada población lo que constituye un elemento importante para trazar estrategias de mejoramiento y/o conservación genética.

Summary

The present study shows the first results of the genetic structure determination of three populations of Cuban autochthonous bovine livestock: Criollo de Cuba, Cebú Cubano and Siboney de Cuba, for the six major milk proteins loci (CASA1, CASAB, CASA2, CASK, LAA and LGB). A total of 150 individuals (50 for each population) were analysed, by means of DNA analysis by PCR-RFLP.The allelic frequencies were calculated for each locus as well as conditions of Hardy-Weinberg equilibrium. The CASA1C and LAAA alelles were identified as evidence of the presence of Bos indicus genes in the three Cuban populations. It was proven that the existence of high variability in each population constitutes an important means to establish strategies for improvement and/or genetic conservation.

Keywords

polimorfismoPCR-RFLP
Type
Research Articles
Information
Animal Genetic Resources/Resources génétiques animales/Recursos genéticos animales , Volume 39 , April 2006 , pp. 15 - 24
Copyright
Copyright © Food and Agriculture Organization of the United Nations 0000

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