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TNF-α and intPLA2 genes' polymorphism in anorexia nervosa

Published online by Cambridge University Press:  24 June 2014

Agnieszka Slopien ,
Filip Rybakowski ,
Monika Dmitrzak-Weglarz ,
Piotr Czerski ,
Joanna Hauser ,
Renata Komorowska-Pietrzykowska  and
Andrzej Rajewski
Agnieszka Slopien*
Affiliation:
Department of Child and Adolescent Psychiatry, University of Medical Sciences, Poznañ, Poland
Filip Rybakowski
Affiliation:
Department of Child and Adolescent Psychiatry, University of Medical Sciences, Poznañ, Poland
Monika Dmitrzak-Weglarz
Affiliation:
Department of Child and Adolescent Psychiatry, University of Medical Sciences, Poznañ, Poland Laboratory of Psychiatric Genetics, University of Medical Sciences, Poznañ, Poland
Piotr Czerski
Affiliation:
Department of Adult Psychiatry, University of Medical Sciences, Poznañ, Poland Laboratory of Psychiatric Genetics, University of Medical Sciences, Poznañ, Poland
Joanna Hauser
Affiliation:
Department of Adult Psychiatry, University of Medical Sciences, Poznañ, Poland Laboratory of Psychiatric Genetics, University of Medical Sciences, Poznañ, Poland
Renata Komorowska-Pietrzykowska
Affiliation:
Department of Child and Adolescent Psychiatry, University of Medical Sciences, Poznañ, Poland
Andrzej Rajewski
Affiliation:
Department of Child and Adolescent Psychiatry, University of Medical Sciences, Poznañ, Poland
*
Agnieszka Slopien, Department of Child and Adolescent Psychiatry, Szpitalna 27/33 Street, 60-572 Poznañ, Poland. Tel: +48 61 8491531; Fax: +48 61 8480392; E-mail: asrs@wp.pl
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Abstract

Objective:

The aim of this study was the assessment of −308G/A tumor necrosis factor (TNF)-α gene polymorphism and intPLA2 gene polymorphism in patients with anorexia nervosa (AN) and healthy controls.

Subjects:

We studied 91 non-related patients with AN and 144 healthy women (blood donors and students). The mean age of women from study group was 18.22 years (SD ± 3.13 years) and from control group was 31.71 years (SD ± 8.22).

Methods:

Gene polymorphisms were studied with the use of polymerase chain reaction-restriction fragment length polymorphism method. TNF-α gene polymorphism consists of G/A substitution in −308 promoter region. IntPLA2 gene polymorphism is related to intron 1, in which restrictive region is found and recognized by BanI enzyme.

Results:

We did not obtain statistically significant differences in the frequency of genotypes and alleles of −308G/A TNF-α polymorphism between the study and control groups (genotypes: P = 0.106, alleles: P = 0.076). We did analogous analysis in the restrictive and bulimic subgroups. We did not observe statistically relevant differences in the frequency of genotypes (P = 0.700) and alleles (P = 0.305). We did not obtain statistically relevant difference in the frequency of genotypes and alleles of intPLA2 gene between the study group and controls (genotypes: P = 0.300, alleles: P = 0.331). We did analogous analysis in both subgroups of AN. We did not observe statistically relevant differences in the frequency of genotypes (P = 0.344) and alleles (P = 0.230).

Conclusions:

There was no statistically relevant trend for the association between TNF-α polymorphism and AN. We did not find association between studied polymorphism of intPLA2 gene and risk of AN.

Keywords

anorexia nervosageneticsintPLA2TNF-α
Type
Original Article
Information
Acta Neuropsychiatrica , Volume 16 , Issue 6 , December 2004 , pp. 290 - 294
Copyright
Copyright © 2004 Blackwell Munksgaard

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