Book contents
- Frontmatter
- Contents
- Contributors
- Preface
- Part I Types of Imaging Reporter Genes
- Part II Enhancing Reporter Gene Imaging Techniques
- Part III Imaging Instrumentations
- Part IV Current Applications of Imaging Reporter Genes
- 8 Reporter Gene Imaging of Cell Signal Transduction
- 9 Gene Therapy and Imaging of Transgene Expression in Living Subjects
- 10 Imaging Regulation of Endogenous Gene Expression in Living Subjects
- 11 Imaging Cell Trafficking and Immune Cell Activation Using PET Reporter Genes
- 12 Imaging of Reporter Genes and Stem Cells
- 13 Clinical Applications of Reporter Gene Technology
- Index
- References
13 - Clinical Applications of Reporter Gene Technology
Published online by Cambridge University Press: 07 September 2010
- Frontmatter
- Contents
- Contributors
- Preface
- Part I Types of Imaging Reporter Genes
- Part II Enhancing Reporter Gene Imaging Techniques
- Part III Imaging Instrumentations
- Part IV Current Applications of Imaging Reporter Genes
- 8 Reporter Gene Imaging of Cell Signal Transduction
- 9 Gene Therapy and Imaging of Transgene Expression in Living Subjects
- 10 Imaging Regulation of Endogenous Gene Expression in Living Subjects
- 11 Imaging Cell Trafficking and Immune Cell Activation Using PET Reporter Genes
- 12 Imaging of Reporter Genes and Stem Cells
- 13 Clinical Applications of Reporter Gene Technology
- Index
- References
Summary
INTRODUCTION
For more than a decade, molecular imaging (MI) has increasingly been used to successfully image gene expression in living animals, thus making significant contributions to the field of gene and cellular gene therapy. However, there has been slow progress in translating these technologies into clinical application, even though there is a real need to develop, test, and validate sensitive and reproducible noninvasive imaging methods that could be repeatedly and safely performed in patients undergoing gene therapy.
New molecular biology technologies now permit rapid determination of expression levels of hundreds of genes from minute tissue samples. These technologies combined with the complete sequencing of the human genome have allowed establishment of a molecular signature for many diseases. Nonetheless, gene expression patterns can change during the course of the pathology and in response to therapy. Such modification of gene expression patterns can alter drug sensitivity. Hence, molecular imaging can play a role in monitoring variation of gene expression during treatment.
Molecular imaging is emerging as a noninvasive technology for in vivo mapping of gene expression and provides promising tools for accelerated progress of molecular medicine (for a review see and references therein). Recently the importance of multimodality imaging has been recognized, allowing imaging of gene expression in intact cultured cells up to noninvasive whole-body imaging, both in animal models and in humans.
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- Information
- Molecular Imaging with Reporter Genes , pp. 297 - 314Publisher: Cambridge University PressPrint publication year: 2010