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  3. Biophysical Labeling Methods in Molecular Biology
  • Cited by 59
Publisher:
Cambridge University Press
Online publication date:
January 2010
Print publication year:
1993
Online ISBN:
9780511629099
DOI:
https://doi.org/10.1017/CBO9780511629099
Subjects:
Life Sciences, Physiology and Biological Physics, Molecular Biology, Biochemistry, and Structural Biology
Information

Book description

Physical labels such as stable nitroxide radicals, luminescent and photochromic chromophores, so-called Mössbauer atoms and electron-dense assemblies of heavy atoms have proved to be effective tools in solving many problems at the molecular level in biological systems. These physical labels are used as 'molecular rulers' to measure the distances between chosen groups and to measure the size, form and microrelief of objects. By providing information about these factors, the label provides information that can help the scientist to understand the structure of membranes, nucleic acids, enzymes and proteins and how they function. This 1993 volume covers all aspects of this field: the theoretical bases, the experimental techniques, and it also shows how to interpret the resulting data. It also critically discusses some recent results obtained with these techniques and gives an analysis of likely developments in the future.

Reviews

"Labeling methods, including fluorescence and spin labeling, are widely used to probe the structure and function of biomolecules....I strongly encourage anyone who wants an intense and well structured introduction into the field of labeling to read Likhenshtein's book. Even the experienced researcher will benefit from it because of the parallel presentation of several labeling methods." Klaus Gawrisch, Analytical Biochemistry

"...should be of use to graduate students and those considering research in biophysical chemistry who want an historical overview of labeling methods." Bruce Eaton, Journal of the American Chemical Society

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