The foregoing statement is as valid now as it was when it was written 27 years ago. Modern studies of Volvox biology began in Richard Starr's laboratory in the 1960s, when William Darden, then a graduate student, demonstrated that axenic cultures of Volvox aureus could be maintained indefinitely in a chemically defined medium that had been developed a few years earlier for culturing other types of algae (Provasoli & Pintner 1959). In such cultures he was able to observe and study all stages in the asexual and sexual life history of V. aureus under controlled conditions (Darden 1966). Later, he and his associates would use this culture system to examine a variety of aspects of V. aureus biology (Darden 1968, 1970, 1971, 1973a,b; 1980; Deason et al. 1969; Darden & Sayers 1969, 1971; Ely & Darden 1972; Tucker & Darden 1972).