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Chapter 3 - Consumables for the IVF Laboratory

Production and Validation, Quality Control, and Bioassays

Published online by Cambridge University Press:  15 April 2021

Kersti Lundin
Sahlgrenska University Hospital, Gothenburg
Aisling Ahlström
Livio Fertility Center, Gothenburg
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Since the birth of Louise Brown in 1978, IVF has evolved from a research-based environment to an established and regulated clinical treatment with around 2 million treatment cycles performed annually worldwide.

Publisher: Cambridge University Press
Print publication year: 2021

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Bolton, VN, Hawes, SM, Taylor, CT, Parsons, JH. Development of spare human preimplantation embryos in vitro: an analysis of the correlations among gross morphology, cleavage rates, and development to the blastocyst. J In Vitro Fert Embryo Transf. 1989;6:3035.CrossRefGoogle Scholar
Ray, BD, McDermott, A, Wardle, PG, et al. In vitro fertilization: fertilization failure due to toxic catheters. J In Vitro Fert Embryo Transf. 1987;4:5861.Google Scholar
Hinduja, I, Peter, J, Menezes, J. Instrument failure in in vitro fertilization: a case report. J In Vitro Fert Embryo Transf. 1985;2:108109.CrossRefGoogle ScholarPubMed
Harper, J, Magli, MC, Lundin, K, Barratt, CLBrison, D. When and how should new technology be introduced into the IVF laboratory? Hum Reprod. 2012;27:303313.Google Scholar
Provoost, V, Tilleman, K, D’Angelo, A, et al. Beyond the dichotomy: a tool for distinguishing between experimental, innovative and established treatment. Hum Reprod. 2014;29:413417.CrossRefGoogle ScholarPubMed
Hardarson, T, Bungum, M, Conaghan, J, et al. Noninferiority, randomized, controlled trial comparing embryo development using media developed for sequential or undisturbed culture in a time–lapse setup. Fertil Steril. 2015;104:14521459.e1–4.CrossRefGoogle ScholarPubMed
Kleijkers, SH, Mantikou, E, Slappendel, E, et al. Influence of embryo culture medium (G5 and HTF) on pregnancy and perinatal outcome after IVF: a multicenter RCT. Hum Reprod. 2016;31:22192230.Google Scholar
Wale, PLGardner, DK. The effects of chemical and physical factors on mammalian embryo culture and their importance for the practice of assisted human reproduction. Hum Reprod Update. 2016;22:222.Google Scholar
Otsuki, J, Nagai, Y, Chiba, K. Peroxidation of mineral oil used in droplet culture is detrimental to fertilization and embryo development. Fertil Steril. 2007;88:741743.Google Scholar
Nijs, M, Franssen, K, Cox, A, Wissmann, D, Ruis, H, Ombelet, W. Reprotoxicity of intrauterine insemination and in vitro fertilization–embryo transfer disposables and products: a 4–year survey. Fertil Steril. 2009;92:527535.Google Scholar
Punt–van der Zalm, JP, Hendriks, JC, Westphal, JR, Kremer, JA, Teerenstra, S, Wetzels, AM. Toxicity testing of human assisted reproduction devices using the mouse embryo assay. Reprod Biomed Online. 2009;18:529535.Google Scholar
Ackerman, SB, Stokes, GL, Swanson, RJ, Taylor, SP, Fenwick, L. Toxicity testing for human in vitro fertilization programs. J In Vitro Fert Embryo Transf. 1985;2:132137.Google Scholar
Bavister, BD, Andrews, JC. A rapid sperm motility bioassay procedure for quality–control testing of water and culture media. J In Vitro Fert Embryo Transf1988;5:6775.Google Scholar
Gardner, DK, Reed, L, Linck, D, Sheehan, C, Lane, M. Quality control in human in vitro fertilization. Semin Reprod Med2005;23:319324.Google Scholar
Delaroche, L, Oger, P, Genauzeau, E. Embryotoxicity testing of IVF disposables: how do manufacturers test? Hum Reprod. 2020;35:283292.Google Scholar
Otsuki, J, Nagai, Y, Chiba, K. Damage of embryo development caused by peroxidized mineral oil and its association with albumin in culture. Fertil Steril. 2009;91:17451749.CrossRefGoogle ScholarPubMed
Hughes, PM, Morbeck, DE, Hudson, SB, Fredrickson, JR, Walker, DL, Coddington, CC. Peroxides in mineral oil used for in vitro fertilization: defining limits of standard quality control assays. J Assist Reprod Genet. 2010;27:8792.Google Scholar
Khan, Z, Wolff, HS, Fredrickson, JR, Walker, DL, Daftary, GS, Morbeck, DE. Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice. Fertil Steril. 2013;99:847854.e2.Google Scholar
Ainsworth, AJ, Fredrickson, JR, Morbeck, DE. Improved detection of mineral oil toxicity using an extended mouse embryo assay. J Assist Reprod Genet. 2017;34:391397.CrossRefGoogle ScholarPubMed
Morbeck, DE, Paczkowski, M, Fredrickson, JR. Composition of protein supplements used for human embryo culture. J Assist Reprod Genet. 2014;31:17031711.CrossRefGoogle ScholarPubMed
Dyrlund, TF, Kirkegaard, K, Poulsen, ET. Unconditioned commercial embryo culture media contain a large variety of non–declared proteins: a comprehensive proteomics analysis. Hum Reprod. 2014;29:24212430.Google Scholar
Fredrickson, J, Krisher, R, Morbeck, DE. The impact of the protein stabilizer octanoic acid on embryonic development and fetal growth in a murine model. J Assist Reprod Genet. 2015;32:15171524.Google Scholar
Schiewe, MC, Schmidt, PM, Bush, M, Wildt, DE. Toxicity potential of absorbed–retained ethylene oxide residues in culture dishes on embryo development in vitro. J Anim Sci1985;60:16101618.Google Scholar
Harrison, KL, Sherrin, DA, Hawthorne, TA, Breen, TM, West, GA, Wilson, LM. Embryotoxicity of micropore filters used in liquid sterilization. J In Vitro Fert Embryo Transf1990;7:347350.Google Scholar
Nagata, Y, Shirakawa, K. Setting standards for the levels of endotoxin in the embryo culture media of human in vitro fertilization and embryo transfer. Fertil Steril1996;65:614619.CrossRefGoogle ScholarPubMed
Dubin, NH, Bornstein, DR, Gong, Y. Use of endotoxin as a positive (toxic) control in the mouse embryo assay. J Assist Reprod Genet1995;12:147152.Google Scholar
Lane, M, Gardner, DK. Differential regulation of mouse embryo development and viability by amino acids. J. Reprod. Fertil. 1997; 109:153164.Google Scholar
Janssens, R, Verheyen, G, Cortvrindt, R. A review of commercial MEA testing and plea for informative MEA certificates of analysis. Abstracts of the 10th Biennial Conference of Alpha, Scientists in Reproductive Medicine, 9–11 May 2014, Antalya, Turkey. Reprod Biomed Online. 2014;28(suppl 1) S2.CrossRefGoogle Scholar

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