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Practical applications of agricultural wastes in poultry feeding in Mediterranean and Middle East regions. Part 1: citrus, grape, pomegranate and apple wastes

  • M. AZIZI (a1), A.R. SEIDAVI (a1), M. RAGNI (a2), V. LAUDADIO (a3) and V. TUFARELLI (a3)...


In the last few decades, there has been growing interest in the use of agricultural wastes as feed ingredients in poultry diet to maximise their potential output. Many wastes of the agroindustry (e.g. citrus, grape, pomegranate and apple processing) have significant antioxidant properties, due to their bioactive compounds (polyphenols, flavonoids). It has been reported that citrus and grape wastes can be successfully included in broilers diets up to 3%, which increases carcass and meat yield and reduces abdominal fat (by 10% on average). Pomegranate can be included up to 2% supplementation and is associated with improved feed efficiency (by 12% in broilers). Apple by-products can be formulated up to 5% in diets for meat-type broilers and up to 10% in laying hens diet to reduce oxidative stress. Therefore, the application of these by-products in poultry diets could combine the positive effects of improving the qualitative characteristics of animal products as well as human health with reducing concerns related with their disposal into the environment. In this review, the findings on the effects of some agricultural wastes, from typically-cultivated products originating in Mediterranean and Middle East regions on poultry performance, carcass characteristics, immune response, plasma constitutes, intestinal microbiota and enzyme activity are reviewed and discussed.


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ABBASI, H., REZAEI, K. and RASHIDI, L. (2008) Extraction of essential oils from the seeds of pomegranate using organic solvents and supercritical CO2. Journal of American Oil Chemists’ Society 85: 83-89.
ABBASI, H., SEIDAVI, A.R., LIU, W. and ASADPOUR, L. (2015) Investigation on the effect of different levels of dried sweet orange (Citrus sinensis) pulp on performance, carcass characteristics and physiological and biochemical parameters in broiler chicken. Saudi Journal of Biological Sciences 22 (2): 139-143.
AHMAD, G.A. (2004) Simultaneous use of apple pomace and molasses as a source of energy for broiler. Indian Journal of Poultry Science 39 (2): 179-181.
AHMED, S.T., MANIRUL ISLAM, M.D., RUBAYET BOSTAMI, A.B.M., MUN, H.S., KIM, Y.J. and YANG, C.J. (2015) Meat composition, fatty acid profile and oxidative stability of meat from broilers supplemented with pomegranate (Punica granatum L.) by products. Food Chemistry 188: 481-488.
ALEFZADEH, T., BOUYEH, M., VAN DEN HOVEN, R., SEIDAVI, A., LAUDADIO, V. and TUFARELLI, V. (2016) Effect of dietary dried orange (Citrus sinensis) peel powder and exogenous multi enzymes on growth and carcass traits and ileal microflora of broiler chickens. Pakistan Journal of Zoology 48 (6): 1891-1896.
AL-HARTHI, M.A. (2017) The effect of olive cake, with or without enzymes supplementation, on growth performance, carcass characteristics, lymphoid organs and lipid metabolism of broiler chickens. Revista Brasileira de Ciência Avícola 19: 83-90.
ALONSO, A.M., GUILLEN, D.A., BARROSO, C.G., PUERTAS, B. and GARCIA, A. (2002) Determination of antioxidant activity of wine byproducts and its correlation with polyphenolic content. Journal of Agricultural and Food Chemistry 50: 5832-5836.
AMER, N., AL-HILFY, E.J. and AL-TAIE, M. (2011) Effect of apple liter contained of apple fibers and gel pectin on body weight, lipid profiles, kidney function and histological structure of kidney in male albino rats. Danish Journal of physiology Science 8 (2): 178-187.
AYHAN, V., ARSLANDURU, A. and OZKAYA, S. (2009) Possibilities of using dried apple pomace in broiler chicken diets. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 15 (5: 669-672.
BAMPIDIS, V.A. and ROBINSON, P.H. (2006) Citrus byproducts as ruminant feeds: A review. Animal Feed Science and Technology 128: 175-217.
BONILLA, F., MAYEN, M., ME´RIDA, J. and MEDINA, M. (1999) Extraction of phenolic compounds from red grape marc for use as food lipid antioxidants. Food Chemistry 66: 209-215.
BOSTAMI, A.B.M.R., AHMED, S.T., ISLAM, M.M., MUN, H.S., KO, S.Y., KIM, S.S. and YANG, C.J. (2015) Growth performance, fecal noxious gas emission and economic efficacy in broilers fed fermented pomegranate by products as residue of fruit industry. International Journal of Advanced Research 3 (3): 102-114.
BRENES, A., VIVEROS, A., GOÑI, I., CENTENO, C., SÁYAGO-AYERDY, S.G., ARIJA, I. and SAURA-CALIXTO, F. (2008) Effect of grape pomace concentrate and vitamin E on digestibility of polyphenols and antioxidant activity in chickens. Poultry Science 87: 307-316.
CHUANG, C., HSU, C., CHAO, C., WEIN, Y., KUO, Y. and HUANG, C. (2006) Fractionation and identification of 9c, 11t, 13t-conjugated linolenic acid as an activator of PPARa in bitter gourd (Momordica charantia L.). Journal of Biomedical Science 13: 763-772.
DE PETERS, E.J., FADEL, J.G. and AROSENA, A. (1997) Digestion kinetics of neutral detergent fiber and chemical composition within some selected byproduct feedstuffs. Animal Feed Science Technology 67: 127-140.
DHAR, P., BHATTACHARYYA, D., BHATTACHARYYA, D.K. and GHOSH, S. (2006) Dietary comparison of conjugated linolenic acid (9cis, 11trans, 13trans) and alpha-tocopherol effects on blood lipids and lipid peroxidation in alloxan induced diabetes mellitus rats. Lipids 41: 49-54.
DORRI, S., TABEIDIAN, S.A., TOGHYANI, M., JAHANIAN, R. and BEHNAMNEJAD, F. (2012) Effect of different levels of grape pomace on immune responses and blood serum oxidative stability broiler chicks. In: 5th Iranian Congress on Animal Science, Dept. Animal Science, Isfahan University of Technology, Isfahan, Iran.
EBRAHIMI, A., ALAW QOTBI, A.A. and SEIDAVI, A. (2013a) The effects of different levels of dried Citrus sinensis peel on broiler carcass quality. Acta Scientiae Veterinariae 41: 1171, 1-8.
EBRAHIMI, A., ALAW QOTBI, A.A., SEIDAVI, >A., LAUDADIO, V. and TUFARELLI, V. (2013b) Effect of different levels of dried sweet orange (Citrus sinensis) peel on broiler chickens growth performance. Archives of Animal Breeding 56 (2): 11-17.
EBRAHIMI, A., ALAW QOTBI, A.A., SEIDAVI, A. and BAHAR, B. (2014) The effects of dietary supplementation of Citrus sinensis peel extract on production and quality parameters of broiler chicken. Journal of Applied Animal Research 42 (4): 445-450.
EBRAHIMI, A., ALAW QOTBI, A.A., SEIDAVI, A.R., EDENS, F.W., LAUDADIO, V. and TUFARELLI, V. (2016) Selected plasma constituents of broiler chickens fed different levels of dried sweet orange (Citrus sinensis) peels. Journal of Animal and Plant Sciences 26 (4): 949-955.
EBRAHIMI, A., SANTINI, A., ALISE, M., POURHOSSEIN, Z., MIRAALAMI, N. and SEIDAVI, A. (2015) Effect of dried Citrus sinensis peel on gastrointestinal microbiota and immune system traits of broiler chickens. Italian Journal of Animal Science 14: 712-717.
GHAEMI, H., NOBAKHT, A. and RAZZAGHZADEH, S. (2014) The effect of apple pulp and multi enzyme on performance and blood parameters in native laying hens. Journal of Farm Animal Nutrition and Physiology 9 (1): 10-21.
GOÑI, I., BRENES, A., CENTENO, C., VIVEROS, A., SAURA-CALIXTO, F., REBOLÉ, A., ARIJA, I. and ESTEVEZ, R. (2007) Effect of dietary grape pomace and vitamin E on growth performance, nutrient digestibility, and susceptibility to meat lipid oxidation in chickens. Po ultry Science 86: 508-516.
GONZALEZ-PARAMÁS, A.M., ESTEBAN-RUANO, S., SANTOS-BUELGA, C., PASCUAL-TERESA, S. and RIVAS-GONZALO, J.C. (2004) Flavanol content and antioxidant activity in winery products. Journal of Agricultural and Food Chemistry 52: 234-238.
HEIDARISAFAR, Z., SADEGH, G., KARIMI, A. and AZIZI, O. (2016) Apple peel waste as a natural antioxidant for heat-stressed broiler chickens. Tropical Animal Health and Production 48 (4): 831-835.
HOSSEINI, S.M., AMOLI, M. and MODARESI, S.J. (2014) Effect of different pomegranate seed pulp levels on performance traits and some blood parameters of broilers. Animal Production Research 3 (2): 29-38.
HOSSEINI-VASHAN, S.J., YAGHOBFAR, A. and GOLIAN, A. (2016) Determination of the effects of organic antioxidants and fat sources on performance, carcass and bone characteristics of broilers under heat stress. Iranian Journal of Animal Science Research 8 (2): 105-114.
IGARASHI, M. and MIYASAWA, T. (2000) Newly recognized cytotoxic effect of conjugated trienoic acids on cultured human tumor cells. Cancer Letters 148: 173-179.
JOSHI, V.K., GUPTA, K., DEVRAJAN, A., LAL, B.B. and ARYA, S.P. (2010) Production and evaluation of fermented apple pomace in the feed of broilers. Journal of Food Science and Technology 37 (6): 609-612.
KARA, K. and KOCAOGLU-GUCLU, B. (2012) The effects of different molting methods and supplementation of grape pomace to the diet of molted hens on post-molt performance, egg quality and peroxidation of egg lipids. Journal of Faculty of Veterinary Medicine, Erciyes University 9: 183-196.
KIM, M.S., YUN, S.H., NA, H.S., PARK, H.J., CHOI, G.C., YANG, S.I. and LEE, J.H. (2013) Chemical compositions and functional characteristics of Korean and imported pomegranate (Punica granatum L.). Korean Journal of Food Preservation 20 (3): 342-347.
KOBA, K., AKAHOSHI, A., YAMASAKI, M., TANAKA, K., YAMADA, K., IWATA, T., KAMEGAI, T., TSUTSUMI, K. and SUGANO, M. (2002) Dietary conjugated linolenic acid in relation to CLA differently modifies body fat mass and serum and liver lipid levels in rats. Lipids 37: 343-350.
LICHOVNIKOVA, M., KALHOTKA, L., ADAM, V., KLEJDUS, B. and ANDERLE, V. (2015) The effects of red grape pomace inclusion in grower diet on amino acid digestibility, intestinal microflora, and sera and liver antioxidant activity in broilers. Turkish Journal of Veterinary and Animal Sciences 39: 406-412.
LOUBA, B.N. (2007) What are the medical properties of pomegranates? Journal of Chinese Clinical Medicine 2: 530-538.
LU, C., ZHANG, Z., GE, X., WANG, Y., ZHOU, X., YOU, X. and ZHANG, Q. (2016) Bio-hydrogen production from apple waste by photosynthetic bacteria HAU-M1. International Journal of Hydrogen Energy 41 (31): 13399-13407.
MATOO, F., BEAT, G., BANDAY, M. and GANAIE, T. (2001) Performance of broilers fed on apple pomace diets supplemented with enzyme(s). Indian Journal of Animal Nutrition 18: 349-352.
MIRDEHGHAN, S.H., RAHEMI, M., MARTÍNEZ-ROMERO, D., GUILLÉN, F., VALVERDE, J.M., ZAPATA, P.J., SERRANO, M. and VALERO, D. (2007) Reduction of pomegranate chilling injury during storage after heat treatment: Role of polyamines. Postharvest Biology and Technology 4 (1): 19-25.
MODARRESI, S.J., FATHI, M.H., NASRI, M.H., DAYANI, O. and RASHIDI, L. (2010) The effect of pomegranate seed pulp feeding on DMI, performance and blood metabolites of Southern Khorasan crossbred goats. Iranian Journal of Animal Science Research 20: 23-31.
MOLINA-ALCAIDE, E. and YÁÑEZ-RUIZ, D.R. (2008) Potential use of olive by-products in ruminant feeding. Animal Feed Science and Technology 147: 247-264.
MOURAO, J.L., PINHEIRO, V.M., PRATES, J.A.M., BESSA, R.J.B., FERREIRA, L.M.A., FONTES, C.M.G.A. and PONTER, P.I.P. (2008) Effect of dietary dehydrated pasture and citrus pulp on the performance and meat quality of broiler chickens. Poultry Science 87: 733-743.
NAZOK, A., REZAEI, M. and SAYYAHZADEH, H. (2010) Effect of different levels of dried citrus pulp on performance, egg quality, and blood parameters of laying hens in early phase of production. Tropical Animal Health and Production 42: 737-742.
NOBAKHT, A. (2013a) Effects of different levels of dried lemon pulp on performance, carcass traits, blood biochemical and immunity parameters of broilers. Iranian Journal of Applied Animal Science 3: 145-151.
NOBAKHT, A. (2013b) The effect of different levels of apple wastes on performance, egg quality and blood parameters of commercial laying hens. Journal of Veterinary Clinical Research 4: 149-166.
OZGAN, A. (2008) Use of grape seed oil in functional egg production. MSc Thesis, Adana (Turkey), University of Cukurova.
POURHOSSEIN, Z., QOTBI, A.A.A. and SEIDAVI, A. (2012) Does different levels of dried Citrus sinensis peel effect on broilers gastrointestinal microbial population? Annals of Biological Research 3 (9): 4474-4479.
POURHOSSEIN, Z., QOTBI, A.A.A., SEIDAVI, A.R., LAUDADIO, V., CENTODUCATI, G. and TUFARELLI, V. (2015) Effect of different levels of dietary sweet orange (Citrus sinensis) peel extract on humoral immune system responses in broiler chickens. Animal Science Journal 86: 105-110.
RAJANI, J., KARIMI TORSHIZI, M.A. and RAHIMI, S.H. (2011) Control of ascites mortality and improved performance and meat shelf-life in broilers using feed adjuncts with presumed antioxidant activity. Animal Feed Science and Technology 170: 239-245.
RUBERTO, G., RENDA, A., DAQUINO, C., AMICO, V., SPATAFORA, C., TRINGALI, C. and TOMMASI, N. (2007) Polyphenols constituents and antioxidant activity of grape pomace from five Sicilian red grape cultivars. Food Chemistry 100: 203-210.
SADIGHI, K. and NOBAKHT, A. (2015) The effect of lemon grape and apple pulps on performance, carcass traits, digestive tract characteristic, intestinal morphology and immune status of broilers. Iranian Journal of Animal Science Research 4 (7): 466-477.
SALEH, H., GOLIAN, A., KERMANSHAHI, H., FARHOOSH, R. and TAHER MIRAKZEHI, R. (2015a) Effect of α-tocopherol acetate, pomegranate peel and extract on performance, nutrient digestibilities and tibia bone calcification in broiler chickens. Research on Animal Production 6 (1):1-11.
SALEH, H., GOLIAN, A., KERMANSHAHI, H., TAHER MIRAKZEHI, M. and AGAH, M.J. (2015b) Effects of natural antioxidant on the immune response, antioxidant enzymes and hematological broilers chickens. Iranian Veterinary Journal 11 (3): 67-126.
SEIDAVI, A., EBRAHIMI, A., ALAW QOTBI, A.A. and GÓMEZ NIETO, J.A. (2015) Effect of different levels of Citrus sinensis peel extract on broiler performance, blood parameters, thyroid gland activity and bone ash. Indian Journal of Animal Sciences 85 (11): 1225-1228.
SZYMCZYK, B. and SZCZUREK, W. (2016) Effect of dietary pomegranate seed oil and linseed oil on broiler chickens performance and meat fatty acid profile. Journal of Animal and Feed Sciences 25: 37-44.
TORRES, J.L., VARELA, B., GARCIA, M.T., CARILLA, J., MATITO, C., CENTELLES, J.J., CASCANTE, M., SORT, X. and BOBET, R. (2002) Valorisation of grape (Vitis vinifera) byproducts. Antioxidant and biological properties of polyphenolic fractions differing in procyanidin composition and flavonol content. Journal of Agricultural and Food Chemistry 50: 7548-7555.
VILLAS-BÔAS, G.S., ESPOSITO, E. and MENDONC, M.M.D. (2003) Bioconversion of apple pomace into a nutritionally enriched substrate by Candida utilis and Pleurotus ostreatus. World Journal of Microbiology and Biotechnology 19: 461-467.
WANG, M.L., SUO, X., GU, J.H., ZHANG, W.W., FANG, Q. and WANG, X. (2008) Influence of grape seed proanthocyanidin extract in broiler chickens: effect on chicken coccidiosis and antioxidant status. Poultry Science 87: 2273-2280.
WILKINSON, J.M. (2011) Re-defining efficiency of feed use by livestock. Animal 5 (7): 1014-1022.
YAMASAKI, M., KITAGAWA, T., KOYANAGI, N., CHUJO, H., MAEDA, H., KOHNO-MURASE, J., IMAMURA, J., TACHIBANA, H. and YAMADA, K. (2006) Dietary effect of pomegranate seed oil on immune function and lipid metabolism in mice. Nutrition 22 (1): 54-59.
YASEEN, A.T., EL-KHOLY, M.E.H., ABD EL-RAZIK, W.M.A. and SOLIMAN, M.H. (2014) Effect of using pomegranate peel extract as feed additive on performance, serum lipids and immunity of broiler chicks. Zagazig Veterinary Journal 42 (1): 87-92.
YILMAZ, Y. and TOLEDO, R.T. (2004) Major flavonoids in grape seeds and skins: Antioxidant capacity of catechin, epicatechin, and gallic acid. Journal of Agricultural and Food Chemistry 52: 255-260.
ZAFAR, F., IDREES, M. and AHMAD, Z. (2005) Use of apple byproducts in poultry rations of broiler chicks in Karachi. Pakistan Journal of Physiology 1: 13-25.


Practical applications of agricultural wastes in poultry feeding in Mediterranean and Middle East regions. Part 1: citrus, grape, pomegranate and apple wastes

  • M. AZIZI (a1), A.R. SEIDAVI (a1), M. RAGNI (a2), V. LAUDADIO (a3) and V. TUFARELLI (a3)...


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