Fall applications of fenarimol on hybrid Bermudagrass (Cynodon dactylon × C. transvaalensis) provide effective preemergence Poa annua (L.) control and suppress Ophiosphaerella spp. pathogens; however, concerns exist for turf injury and root growth restrictions. Two 60-d greenhouse studies were conducted to evaluate the effects of fenarimol at 0, 1.6, and 3.2 kg ai/ha per 30 d with and without trinexapac-ethyl (TE) at 0.017 kg ai ha/15 d on ‘TifEagle’ Bermudagrass. Turf color was enhanced by TE 14 d after initial treatment (DAIT) and was continually superior throughout the experiment. Fenarimol at 3.2 kg/ha per 30 d decreased turf color 14 DAIT, but was similar to nontreated turf on all other observation dates. Increased fenarimol rates applied twice caused approximately 10% injury at 42, 49, and 56 d after treatment; however, injury was acceptable after initial and repeat applications. TE reduced clipping yield an average 39% from six sampling dates. Initial fenarimol applications (without TE) reduced clippings by 37% 20 DAIT and repeated applications reduced clippings 40, 50, and 60 DAIT. Increased fenarimol rate linearly decreased root mass for turf treated with and without TE; however, Bermudagrass receiving TE averaged 23% enhanced root mass 60 DAIT over all fenarimol rates. Bermudagrass receiving fenarimol at 0, 1.6, and 3.2 kg/ ha per 30 d with TE averaged 27, 24, and 16% higher root mass, respectively, compared to turf receiving fenarimol without TE. Treatments had no influence on root length. Results indicate that two consecutive fenarimol applications at 1.6 and 3.2 kg/ha per 30 d may cause minor injury to TifEagle Bermudagrass and restrict root growth. Repeated TE applications, however, could decrease injury from fenarimol and enhance rooting relative to fenarimol applied exclusively.