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Rapid Assay Evaluation of Plant Response to Protoporphyrinogen Oxidase (Protox)-Inhibiting Herbicides

  • Jeanne S. Falk (a1), Kassim Al-Khatib (a1) and Dallas E. Peterson (a1)

Abstract

Protoporphyrinogen oxidase (protox)-inhibiting herbicides damage cell membranes, resulting in electrolyte leakage. A whole-plant dose-response study and a rapid assay that measured electrolyte leakage was used to determine the response of wild mustard, soybean, and protox inhibitor–susceptible and protox inhibitor–resistant common waterhemp to increasing doses of three protox inhibitors: acifluorfen, fomesafen, and sulfentrazone. For the dose-response study, whole plants were treated with the three protox-inhibitor herbicides. Electroconductivity assay 1 consisted of cutting discs from leaf tissue and submerging them in an incubation medium containing concentrations of acifluorfen, fomesafen, or sulfentrazone. In electroconductivity assay 2, the entire leaf was treated with solutions containing acifluorfen, fomesafen, or sulfentrazone. The whole-plant dose-response study showed increasing visible injury with increasing herbicide rates for all species and all herbicides. The order of visible injury was wild mustard > susceptible common waterhemp > resistant common waterhemp > soybean. In assay 1, electrolyte leakage from leaf discs treated with acifluorfen or fomesafen increased with increasing herbicide concentrations, and was similar for all species. In contrast, electrolyte leakage from leaf discs treated with sulfentrazone did not increase with increasing herbicide concentrations for any species. In assay 2, only wild mustard leaf discs increased in electrolyte leakage with increasing herbicide rates of acifluorfen, fomesafen, and sulfentrazone and followed the regression curves established by the whole-plant dose-response study. However, assay 2 was not able to distinguish between susceptible wild mustard and tolerant soybean, or between susceptible and resistant waterhemp.

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Corresponding author

Corresponding author's E-mail: khatib@ksu.edu

References

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Bailey, W. A., Wilson, H. P., and Hines, T. E. 2002. Response of potato (Solanum tuberosum) and selected weeds to sulfentrazone. Weed Technol. 16:651658.
Becerril, J. M. and Duke, S. O. 1989. Protoporphyrin IX content correlates with activity of photobleaching herbicides. Plant Physiol. 90:11751181.
Blum, A. and Ebercon, A. 1981. Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Sci. 21:4347.
Choi, J. S., Lee, H. J., Hwang, I. T., Pyon, J. Y., and Cho, K. Y. 1999. Differential susceptibilities of wheat and barley to diphenyl ether herbicide oxyfluorfen. Pest. Biochem. Physiol. 65:6272.
Dayan, F. E., Green, H. M., Weete, J. D., and Hancock, H. G. 1996a. Postemergence activity of sulfentrazone: effects of surfactants and leaf surfaces. Weed Sci. 44:797803.
Dayan, F. E., Weete, J. D., Duke, S. O., and Hancock, H. G. 1997. Soybean (Glycine max) cultivar differences in response to sulfentrazone. Weed Sci. 45:634641.
Dayan, F. E., Weete, J. D., and Hancock, H. G. 1996b. Physiological basis for differential sensitivity to sulfentrazone by sicklepod (Senna obtusifolia) and coffee senna (Cassia occidentalis). Weed Sci. 44:1217.
Duke, S. O. and Kenyon, W. H. 1993. Peroxidizing activity determined by cellular leakage. in Boger, P. and Sandman, G., eds. Target Assays for Modern Herbicides and Related Phytotoxic Compounds. Boca Raton, FL: Lewis. Pp. 6166.
Duke, S. O., Lydon, J., and Paul, R. N. 1989. Oxadiazon activity is similar to that of p-nitro-diphenyl ether herbicides. Weed Sci. 37:152160.
Fadayomi, O. and Warren, G. F. 1976. The light requirement for herbicidal activity of diphenyl ethers. Weed Sci. 24:598600.
Fadayomi, O. and Warren, G. F. 1977. Uptake and translocation of nitrofen and oxyfluorfen. Weed Sci. 25:111114.
Falk, J. S., Shoup, D. E., Al-Khatib, K., and Peterson, D. E. 2004. Survey of common waterhemp response to protox- and ALS-inhibiting herbicides in northeast Kansas. Proc. West. Soc. Weed Sci. 57:73.
Jacobs, J. M. and Jacobs, N. J. 1993. Porphyrin accumulation and export by isolated barley (Hordeum vulgare) plastids. Plant Physiol. 101:11811187.
Jacobs, J. M., Jacobs, N. J., Sherman, T. D., and Duke, S. O. 1991. Effect of diphenyl herbicides on oxidation of protoporphrinogen to protoporphyrin in organellar and plasma membrane enriched fractions of barley. Plant Physiol. 97:197203.
Johnson, W. O., Kollman, G. E., Swithenbank, C., and Yih, R. Y. 1978. RH-6201 (Blazer): a new broad spectrum herbicide for postemergence use in soybeans. J. Agric. Food Chem. 26:285286.
Kenyon, W. H., Duke, S. O., and Vaughn, K. C. 1985. Sequential of effects of acifluorfen on physiological and ultrastructural parameters in cucumber cotyledon discs. Pestic. Biochem. Physiol. 24:240250.
Knowles, N. R. and Knowles, L. O. 1989. Correlations between electrolyte leakage and degree of saturation of polar lipids from aged potato (Solanum tuberosum L.) tuber tissue. Ann. Bot. 63:331338.
Koo, S. J., Neal, J. C., and Di Tomaso, J. M. 1994. Quinclorac-induced electrolyte leakage in seedling grasses. Weed Sci. 42:17.
Krausz, R. F., Kapusta, G., and Matthews, J. L. 1998. Sulfentrazone for weed control in soybeans (Glycine max). Weed Technol. 12:684689.
Lehnen, L. P., Sherman, T. D., Becerril, J. M., and Duke, S. O. 1990. Tissue and cellular localization of acifluorfen-induced porphyrins in cucumber cotyledons. Pestic. Biochem. Physiol. 37:239248.
Li, J., Smeda, R. J., Nelson, K. A., and Dayan, F. E. 2004. Physiological basis for resistance to diphenyl ether herbicides in common waterhemp (Amaranthus rudis). Weed Sci. 52:333338.
Li, Z., Walker, R. H., Wehtje, G., and Hancock, H. G. 2000. Using electrolyte leakage to detect soybean (Glycine max) cultivars sensitive to sulfentrazone. Weed Technol. 14:699704.
Liu, X. and Huang, B. 2000. Heat stress injury in relation to membrane peroxidation in creeping bentgrass. 40:503510.
Matringe, M., Camadro, J. M., Labette, P., and Scalla, R. 1989. Protoporphyrinogen oxidase as a molecular target for diphenyl ether herbicides. Biochem. J. 260:231235.
Matsumoto, H. and Duke, S. O. 1990. Acifluorfen-methyl effects on prophyrin synthesis in Lemna pausicostata Hegelm. J. Agric. Food Chem. 38:20662071.
Matsunaka, S. 1969. Acceptor of light energy in photoactivation of diphenylether herbicides. J. Agric. Food. Chem. 17:171175.
Nunes, M. E. S. and Smith, G. R. 2003. Electrolyte leakage assay capable of quantifying freezing resistance in rose clover. Crop Sci. 43:13491357.
Patzoldt, W. L., Hager, A. G., and Tranel, P. J. 2002. An Illinois waterhemp biotype with resistance to PPO, ALS, and PSII inhibitors. Proc. North Cent. Weed Sci. Soc. 57:161.
Peterson, D. E., Regehr, D. L., Thompson, C. R., and Al-Khatib, K. 2001. Herbicide Mode of Action. Publication C-715. Manhattan, KS: Kansas Cooperative Extension Service. Pp. 1415.
Ritter, R. L. and Coble, H. D. 1981a. Penetration, translocation, and metabolism of acifluorfen in soybean (Glycine max), common ragweed (Ambrosia artemisiifolia), and common cocklebur (Xanthium pensylvanicum). Weed Sci. 29:474480.
Ritter, R. L. and Coble, H. D. 1981b. Influence of temperature and relative humidity on the activity of acifluorfen. Weed Sci. 29:480485.
Sherman, T. D., Becerril, J. M., Matsumoto, H., Duke, M. V., Jacobs, J. M., Jacobs, N. J., and Duke, S. O. 1991. Physiological basis for differential sensitivities of plant species to protoporphyrinogen oxidase-inhibiting herbicides. Plant Physiol. 97:280287.
Shoup, D. E., Al-Khatib, K., and Peterson, D. E. 2003. Common waterhemp (Amaranthus rudis) resistance to protoporphyrinogen oxidase-inhibiting herbicides. Weed Sci. 51:145150.
Sweat, J. K., Horak, M. J., Peterson, D. E., Lloyd, R. W., and Boyer, J. E. 1998. Herbicide efficacy on four Amaranthus species in soybean (Glycine max). Weed Sci. 12:315321.
Unland, R. D., Al-Khatib, K., and Peterson, D. E. 1999. Interactions between imazamox and diphenylethers. Weed Sci. 47:462466.
Vanstone, D. E. and Stobbe, E. H. 1977. Electrolytic conductivity—a rapid measure of herbicide injury. Weed Sci. 25:352354.
Vencill, W. K. 2002. Herbicide Handbook. 8th ed. Lawrence, KS. Weed Science Society of America. 477 p.
Wilson, D. E., Nissen, S. J., and Thompson, A. 2002. Potato (Solanum tuberosum) variety and weed response to sulfentrazone and flumioxazin. Weed Technol. 16:567574.
Witkowski, D. A. and Halling, B. P. 1989. Inhibition of plant protoporphyrinogen oxidase by herbicide acifluorfen-methyl. Plant Physiol. 90:12391242.
Yanase, D., Andoh, A., and Yasudomi, N. 1990. A new simple bioassay to evaluate photosynthetic electron-transport inhibition utilizing paraquat phytotoxicity. Pestic. Biochem. Physiol. 38:9298.

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