Skip to main content Accessibility help
×
Home

Evolved Resistance to Glyphosate in Junglerice (Echinochloa colona) from the Tropical Ord River Region in Australia

  • Todd A. Gaines (a1), Andrew Cripps (a2) and Stephen B. Powles (a1)

Abstract

The objective of this study was to determine whether a junglerice population from the tropical Ord River region of northwest Australia was glyphosate resistant, and whether alternative herbicides labeled for junglerice control were still effective. Seed samples collected from the field site were initially screened with glyphosate in the glasshouse, and surviving individuals were self-pollinated for subsequent glyphosate dose-response studies. Glyphosate resistance was confirmed, as the suspected resistant population was found to be 8.6-fold more resistant to glyphosate than a susceptible population based on survival (LD50 of 3.72 kg ha−1), and 5.6-fold more resistant based on biomass reduction (GR50 of 1.16 kg ha−1). The glyphosate-resistant population was susceptible to label-recommended doses of all other herbicides assessed, including three acetyl-CoA carboxylase (ACC) –inhibiting herbicides (fluazifop-P, haloxyfop, and sethoxydim), two acetolactate synthase (ALS) –inhibiting herbicides (imazamox and sulfometuron), paraquat, and glufosinate. Glyphosate resistance has previously evolved in numerous species found in glyphosate-resistant cropping systems, no-till chemical fallow, fence line, and perennial crop situations. Here we report the evolution of glyphosate resistance in a cropping system that included annual tillage. The evolution of glyphosate resistance in junglerice from a tropical cropping system further demonstrates the need for improved glyphosate stewardship practices globally.

El objetivo de este estudio fue determinar si una población de Echinochloa colona de la región tropical de Ord River en el noroeste de Australia era resistente a glyphosate, y si herbicidas alternativos con etiqueta para el control de E. colona eran todavía efectivos. Muestras de semillas colectadas en el campo fueron inicialmente tratadas con glyphosate en una invernadero, y los individuos sobrevivientes fueron autopolinizados para estudios posteriores de respuesta a dosis con glyphosate. La resistencia a glyphosate fue confirmada, al determinar que la población que se sospechaba resistente fue 8.6 veces más resistente a glyphosate que la población susceptible según la sobrevivencia (LD50 de 3.72 kg ha−1) y 5.6 veces más resistentes según la reducción de biomasa (GR50 de 1.16 kg ha−1). La población resistente a glyphosate fue susceptible a las dosis recomendadas en etiqueta de todos los demás herbicidas evaluados, incluyendo tres herbicidas inhibidores de acetyl-CoA carboxylase (ACC) (fluazifop-P, haloxyfop y sethoxydim), dos herbicidas inhibidores de acetolactate synthase (ALS) (imazamox y sulfometuron), paraquat y glufosinate. La resistencia a glyphosate ha evolucionado previamente en numerosas especies que se encuentran en sistemas con cultivos resistentes a glyphosate, en barbechos químicos con cero labranza, en cercas y en situaciones con cultivos perennes. Aquí reportamos la evolución de resistencia a glyphosate en un sistema de cultivos que incluía labranza anual. La evolución de resistencia a glyphosate en E. colona en un sistema de cultivos tropical demuestra aún más la necesidad de mejorar las prácticas de buen manejo de glyphosate globalmente.

Copyright

Corresponding author

Corresponding author's E-mail: todd.gaines@uwa.edu.au

References

Hide All
Alarcón-Reverte, R., García, A., and Fischer, A. J. 2012. Evaluating mechanisms of glyphosate resistance in junglerice from California. Weed Sci. Soc. Am. Abstr. 52:434.
Binkholder, K. M., Fresenburg, B. S., Teuton, T. C., Xiong, X., and Smeda, R. J. 2011. Selection of glyphosate-resistant annual bluegrass (Poa annua) on a golf course. Weed Sci. 59:286289.
Cook, T., Storrie, A., Moylan, P., and Adams, B. 2008. Field testing of glyphosate-resistant awnless barnyard grass (Echinochloa colona) in northern NSW. Pages 2023. In van Klinken, R. D., et al. (2008). Proceedings of the 16th Australian Weeds Conference. Queensland Weeds Society, Brisbane, Queensland.
de Carvalho, L. B., Cruz-Hipolito, H., Gonzalez-Torralva, F., da Costa Aguiar Alves, P. L., Christoffoleti, P. J., and de Prado, R. 2011. Detection of sourgrass (Digitaria insularis) biotypes resistant to glyphosate in Brazil. Weed Sci. 59:171176.
Dickson, J. W., Scott, R. C., Burgos, N. R., Salas, R. A., and Smith, K. L. 2011. Confirmation of glyphosate-resistant Italian ryegrass (Lolium perenne ssp. multiflorum) in Arkansas. Weed Technol. 25:674679.
Dolman, F., Malone, J., Boutsalis, P., Storrie, A., and Preston, C. 2009. Mechanisms of glyphosate resistance in Echinochloa colona from Australia. Weed Sci. Soc. Am. Abstr. 49:286.
Duke, S. O. and Powles, S. B. 2008. Glyphosate: a once-in-a-century herbicide. Pest Manag. Sci. 64:319325.
Gaines, T. A., Ward, S. M., Bukun, B., Preston, C., Leach, J. E., and Westra, P. 2012. Interspecific hybridization transfers a previously unknown glyphosate resistance mechanism in Amaranthus species. Evol. Appl. 5:2938.
Heap, I. 2011. The International Survey of Herbicide Resistant Weeds. http://www.weedscience.com. Accessed: February 24, 2011.
Knezevic, S. Z., Streibig, J. C., and Ritz, C. 2007. Utilizing R software package for dose-response studies: The concept and data analysis. Weed Technol. 21:840848.
Light, G. G., Mohammed, M. Y., Dotray, P. A., Chandler, J. M., and Wright, R. J. 2011. Glyphosate-resistant common waterhemp (Amaranthus rudis) confirmed in Texas. Weed Technol. 25:480485.
Mueller, T. C., Barnett, K. A., Brosnan, J. T., and Steckel, L. E. 2011. Glyphosate-resistant goosegrass (Eleusine indica) confirmed in Tennessee. Weed Sci. 59:562566.
Norsworthy, J. K., Riar, D., Jha, P., and Scott, R. C. 2011. Confirmation, control, and physiology of glyphosate-resistant giant ragweed (Ambrosia trifida) in Arkansas. Weed Technol. 25:430435.
Powles, S. B. 2008. Evolved glyphosate-resistant weeds around the world: Lessons to be learnt. Pest Manag. Sci. 64:360365.
Powles, S. B., Lorraine-Colwill, D. F., Dellow, J. J., and Preston, C. 1998. Evolved resistance to glyphosate in rigid ryegrass (Lolium rigidum) in Australia. Weed Sci. 46:604607.
Powles, S. B. and Yu, Q. 2010. Evolution in action: Plants resistant to herbicides. Annu. Rev. Plant Biol. 61:317347.
Pratley, J., Urwin, N., Stanton, R., Baines, P., Broster, J., Cullis, K., Schafer, D., Bohn, J., and Krueger, R. 1999. Resistance to glyphosate in Lolium rigidum. I. Bioevaluation. Weed Sci. 47:405411.
R Development Core Team [R 2010]. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0. http://www.R-project.org. Accessed: February 24, 2011.
Riar, D. S., Norsworthy, J. K., Johnson, D. B., Scott, R. C., and Bagavathiannan, M. 2011. Glyphosate resistance in a johnsongrass (Sorghum halepense) biotype from Arkansas. Weed Sci. 59:299304.
Smith, A., Pollock, D., Palmer, D., and Price, A. 2007. Ord River Irrigation Area (ORIA) groundwater drainage and discharge evaluation: Survey of groundwater quality 2006. CSIRO Land and Water Science Report 44/07. http://www.csiro.au. Accessed April 23, 2012.
Storrie, A., Cook, T., Boutsalis, P., Penberthy, D., and Moylan, P. 2008. Glyphosate resistance in awnless barnyard grass (Echinochloa colona (L.) Link) and its implications for Australian farming systems. Pages 74–74. In van Klinken, R. D., et al. (2008). Proceedings of the 16th Australian Weeds Conference. Queensland Weeds Society, Brisbane, Queensland.
Tabacchi, M., Mantegazza, R., Spada, A., and Ferrero, A. 2006. Morphological traits and molecular markers for classification of Echinochloa species from Italian rice fields. Weed Sci. 54:10861093.
Thornby, D. F. and Walker, S. R. 2009. Simulating the evolution of glyphosate resistance in grains farming in northern Australia. Ann. Bot. 104:747756.
Werth, J., Thornby, D., Walker, S., Charles, G., and McDonald, C. 2008. Species shift and resistance: Challenges for Australian cotton systems. Pages 2023. In van Klinken, R. D., et al. (2008). Proceedings of the 16th Australian Weeds Conference. Queensland Weeds Society, Brisbane, Queensland.
Zelaya, I. A., Owen, M. D. K., and VanGessel, M. J. 2007. Transfer of glyphosate resistance: Evidence of hybridization in Conyza (Asteraceae). Am. J. Bot. 94:660673.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed