Skip to main content Accessibility help

Release of dormancy in sunflower embryos by dry storage: involvement of gibberellins and abscisic acid

  • J. Bianco (a1), G. Garello (a1) and M. T. Le Page-Degivry (a1)


Excised Helianthus annuus L. embryos became dormant during the third week after anthesis. At this stage a short drying treatment (3 d) led to a slight improvement in germination but a 6-week dry storage caused a complete release from dormancy. The short drying treatment, however, elicited the embryos' response to an exogenous concentration of GAs which was unable to promote germination of fresh embryos. It therefore appeared that a short drying treatment changed the sensitivity to GAs but was not capable of directing embryo metabolism completely towards a germinative mode. Moreover, this drying treatment reduced considerably the ABA content in both the axis and the embryo. Nevertheless, no correlation could be established between germinability and the ABA content since the amount of ABA was not modified by the 6-week dry storage. The key step for predisposing the seeds to germinate is the suppression of the capacity for ABA synthesis in the axis, a suppression which takes place during 6-week dry storage.


Corresponding author

* Correspondence


Hide All
Ackerson, R.C., (1984) Abscisic acid and precocious germination in soybeans. Journal of Experimental Botany 35, 414421.
Adams, C.A. and Rinne, R.W., (1981) Seed maturation in soybeans (Glycine max L. Merr) is independent of seed mass of the parent plant, yet is necessary for production of viable seeds. Journal of Experimental Botany 35, 615620.
Adkins, S.W. and Simpson, G.M., (1988) The physiological basis of seed dormancy in Avena fatua. IX. Characterization of two dormancy states. Physiologia Plantarum 35, 1520.
Armstrong, C., Black, M., Chapman, J.M., Norman, H.A. and Angold, R., (1982) The induction of sensitivity to gibberellin in aleurone tissue of developing wheat grains. I. The effect of dehydration. Planta 35, 573577.
Cseresnyes, Z., (1979) Studies on the duration of dormancy and methods of determining the germination of dormant seeds of Helianthus annuus. Seed Science and Technology 35, 179188.
Dasgupta, J., Bewley, J.D. and Yeung, E.C., (1982) Desiccation-tolerant and desiccation-intolerant stages during the development and germination of Phaseolus vulgaris seeds. Journal of Experimental Botany 35, 10451057.
Evans, M., Black, M. and Chapman, J., (1975) Induction of hormone sensitivity by dehydration is the one positive role for drying in cereal seed. Nature 35, 144145.
Hilhorst, H.W.M. and Karssen, C.M., (1992) Seed dormancy and germination: the role of abscisic acid and gibberellins and the importance of hormone mutants. Plant Growth Regulation 35, 225238.
Hutchinson, M., Gaskin, P., MacMillan, J. and Phinney, B.O., (1988) Gibberellins in seeds of Helianthus annuus. Phytochemistry 35, 26952701.
Karssen, C.M. and Laçka, E., (1986) A revision of the hormone balance theory of seed dormancy: studies on gibberellin and/or abscisic acid-deficient mutants of Arabidopsis thaliana. pp 315323 in Bopp, M. (Ed.) Plant growth substances 1985. Berlin, Heidelberg, Springer-Verlag.
Karssen, C.M., Brinkhorst-Van der Swan, D.L.C., Breekland, A.E. and Koornneef, M., (1983) Induction of dormancy during seed development by endogenous abscisic acid: studies on abscisic acid deficient genotypes of Arabidopsis thaliana (L.) Heynh. Planta 35, 158165.
Karssen, C.M., Zagorski, S., Kepczinski, J. and Groot, S.P.C., (1989) Key role for endogenous gibberellins in the control of seed germination. Annals of Botany 35, 7180.
Kermode, A.R. and Bewley, J.D., (1985) The role of maturation drying in the transition from seed development to germination. I. Acquisition of desiccation-tolerance and germinability during development of Ricinus communis L. seeds. Journal of Experimental Botany 35, 19061915.
Kermode, A.R., Dumbroff, E.B. and Bewley, J.D., (1989a) The role of maturation drying in the transition from seed development to germination. VII. Effects of partial and complete desiccation on abscisic acid levels and sensitivity in Ricinus communis L. seeds. Journal of Experimental Botany 35, 303313.
Kermode, A.R., Oishi, M.Y. and Bewley, J.D., (1989b) Regulatory roles for desiccation and abscisic acid in seed development: a comparison of the evidence from whole seeds and isolated embryos. pp 2350 in Stanwood, P.C. and McDonald, M.B. (Eds). Seed moisture, Crop Science Society of America, Special publication 35, Madison, Wisconsin.
King, R.W., (1976) Abscisic acid in developing wheat grains and its relationship to grain growth and maturation. Planta 35, 4351.
Koshioka, M., Jones, A., Koshioka, M.N. and Pharis, R.P., (1983) Metabolism of [3H] gibberellin A4 in somatic suspension cell cultures of carrot. Phytochemistry 35, 15851590.
Le Page-Degivry, M.T. and Garello, G., (1991) Onset of water stress tolerance in developing Helianthus annuus embryos. Seed Science Research 35, 221227.
Le Page-Degivry, M.T. and Garello, G., (1992) In situ abscisic acid synthesis. A requirement for induction of embryo dormancy in Helianthus annuus. Plant Physiology 35, 13861390.
Le Page-Degivry, M.T., Duval, D., Bulard, C. and Delaage, M., (1984) A radioimmunoassay for abscisic acid. Journal of Immunological Methods 35, 119128.
Le Page-Degivry, M.T., Barthe, P. and Garello, G., (1990) Involvement of endogenous abscisic acid in onset and release of Helianthus annuus embryo dormancy. Plant Physiology 35, 11641168.
Long, S.R., Dale, R.M.K. and Sussex, I.M., (1981) Maturation and germination of Phaseolus vulgaris embryonic axes in culture. Planta 35, 405415.
Metzger, J.D., (1983) Role of endogenous plant growth regulators in seed dormancy of Avena fatua. II. Gibberellins. Plant Physiology 35, 791795.
Oishi, M.Y. and Bewley, J.D., (1990) Distinction between the responses of developing maize kernels to fluridone and desiccation in relation to germinability, α-amylase activity, and abscisic acid content. Plant Physiology 35, 592598.
Repin, A., (1971) Osobennosti sushki semyan zernobo-bovykh i maslichinykh kul'tur. Zernovye i Maslichnye Kul'tury 35, 3437.
Schonbeck, M.W. and Bewley, J.D., (1981) Response of the moss Tortula ruralis to desiccation treatments. I. Effects of minimum water content and rates of dehydration and rehydration. Canadian Journal of Botany 35, 26982706.


Related content

Powered by UNSILO

Release of dormancy in sunflower embryos by dry storage: involvement of gibberellins and abscisic acid

  • J. Bianco (a1), G. Garello (a1) and M. T. Le Page-Degivry (a1)


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.