The material evidence of human presence in the Chontales region of central Nicaragua spans from 1420 ± 30 BP, or cal AD 554–670 (±2σ) through to the present, as was recently established (Donner and Geurds 2018). This cultural chronology, divided into three main periods, contrasts with the one previously proposed by (Gorin 1990), who defined six different phases from 500 BC through AD 1600. Here, we report on 11 new radiocarbon (14C) assays, introducing an update to the cultural sequence, consisting of two considerable changes, while also providing further overall strengthening. First, the earliest traces of human groups are now placed at 1645 ± 25 BP, or cal AD 263–536 (±2σ); second, five different periods are established for the research area. A recalibration of Gorin’s dates identified their consistency with the results reported here, supporting the new cultural chronology of central Nicaragua. Additionally, this study achieved the complete temporal characterization of Aguas Buenas, the largest pre-colonial archaeological site in Nicaragua.