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Imaging methodologies and applications for nutrition research: what can functional MRI offer?

  • Susan T. Francis (a1) and Sally Eldeghaidy (a1) (a2)
  • Please note a correction has been issued for this article.


Food intake is influenced by a complex regulatory system involving the integration of a wide variety of sensory inputs across multiple brain areas. Over the past decade, advances in neuroimaging using functional MRI (fMRI) have provided valuable insight into these pathways in the human brain. This review provides an outline of the methodology of fMRI, introducing the widely used blood oxygenation level-dependent contrast for fMRI and direct measures of cerebral blood flow using arterial spin labelling. A review of fMRI studies of the brain's response to taste, aroma and oral somatosensation, and how fat is sensed and mapped in the brain in relation to the pleasantness of food, and appetite control is given. The influence of phenotype on individual variability in cortical responses is addressed, and an overview of fMRI studies investigating hormonal influences (e.g. peptide YY, cholecystokinin and ghrelin) on appetite-related brain processes provided. Finally, recent developments in MR technology at ultra-high field (7 T) are introduced, highlighting the advances this can provide for fMRI studies to investigate the neural underpinnings in nutrition research. In conclusion, neuroimaging methods provide valuable insight into the mechanisms of flavour perception and appetite behaviour.

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* Corresponding author: Dr S. Francis, email


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1. Ogawa, S, Lee, TM, Kay, AR et al. (1990) Brain magnetic resonance imaging with contrast dependent on blood oxygenation. Proc Natl Acad Sci USA 87, 98689872.
2. Okada, YC (1983) Inferences concerning anatomy and physiology of the human brain based on its magnetic field. Il Nuovo Cimento D 2, 379409.
3. Thulborn, KR, Waterton, JC, Matthews, PM et al. (1982) Oxygenation dependence of the transverse relaxation time of water protons in whole blood at high field. Biochim Biophys Acta 714, 265270.
4. Buxton, RB, Uludag, K, Dubowitz, DJ et al. (2004) Modeling the hemodynamic response to brain activation. Neuroimage 23, S220S233.
5. Hoge, RD, Atkinson, J, Gill, B et al. (1999) Investigation of BOLD signal dependence on cerebral blood flow and oxygen consumption: the deoxyhemoglobin dilution model. Magn Reson Med 42, 849863.
6. Edelman, RR, Siewert, B, Darby, DG et al. (1994) Qualitative mapping of cerebral blood flow and functional localization with echo-planar MR imaging and signal targeting with alternating radio frequency. Radiology 192, 513520.
7. Kim, SG (1995) Quantification of relative cerebral blood flow change by flow-sensitive alternating inversion recovery (FAIR) technique: application to functional mapping. Magn Reson Med 34, 293301.
8. Wu, W-C, Fernandez-Seara, M, Detre, JA et al. (2007) A theoretical and experimental investigation of the tagging efficiency of pseudocontinuous arterial spin labeling. Magn Reson Med 58, 10201027.
9. Buxton, RB, Wong, EC & Frank, LR (1998) Dynamics of blood flow and oxygenation changes during brain activation: the balloon model. Magn Reson Med 39, 855864.
10. Silva, AC, Lee, SP, Yang, G et al. (1999) Simultaneous blood oxygenation leveldependent and cerebral blood flow functional magnetic resonance imaging during forepaw stimulation in the rat. J Cerebr Blood F Met 19, 871879.
11. Duong, TQ, Kim, DS, Ugurbil, K et al. (2000) Spatiotemporal dynamics of the BOLD fMRI signals: toward mapping submillimeter cortical columns using the early negative response. Magn Reson Med 44, 231242.
12. Purdon, PL & Weisskoff, RM (1998) Effect of temporal autocorrelation due to physiological noise and stimulus paradigm on voxel-level false-positive rates in fMRI. Hum Brain Mapp 6, 239249.
13. Small, DM, Zald, DH, Jones-Gotman, M et al. (1999) Human cortical gustatory areas: a review of functional neuroimaging data. Neuroreport 10, 713.
14. Small, DM, Jones-Gotman, M, Zatorre, RJ et al. (1997) Flavor processing: more than the sum of its parts. Neuroreport 8, 39133917.
15. Veldhuizen, MG, Albrecht, J, Zelano, C et al. (2011) Identification of human gustatory cortex by activation likelihood estimation. Hum Brain Mapp 32, 22562266.
16. Francis, S, Rolls, ET, Bowtell, R et al. (1999) The representation of pleasant touch in the brain and its relationship with taste and olfactory areas. Neuroreport 10, 453459.
17. O'Doherty, J, Rolls, ET, Francis, S et al. (2001) Representation of pleasant and aversive taste in the human brain. J Neurophysiol 85, 13151321.
18. Small, DM, Gregory, MD, Mak, YE et al. (2003) Dissociation of neural representation of intensity and affective valuation in human gustation. Neuron 39, 701711.
19. O'Doherty, JP, Dayan, P, Friston, K et al. (2003) Temporal difference models and reward-related learning in the human brain. Neuron 38, 329337.
20. Kringelbach, ML, de Araujo, IET & Rolls, ET (2004) Taste-related activity in the human dorsolateral prefrontal cortex. Neuroimage 21, 781788.
21. O'Doherty, J, Rolls, E, Francis, S et al. (2001) Representation of pleasant and aversive taste in the human brain. J Neurophysiol 85, 13151321.
22. de Araujo, IE, Kringelbach, M, Rolls, E et al. (2003) Human cortical responses to water in the mouth, and the effects of thirst. J Neurophysiol 90, 18651876.
23. Ogawa, H, Wakita, M, Hasegawa, K et al. (2005) Functional MRI detection of activation in the primary gustatory cortices in humans. Chem Senses 30, 583592.
24. Faurion, A, Cerf, B, Van De Moortele, PF et al. (1999) Human taste cortical areas studied with functional magnetic resonance imaging: evidence of functional lateralization related to handedness. Neurosci Lett 277, 189192.
25. O'Doherty, J, Rolls, ET, Francis, S et al. (2000) Sensory-specific satiety-related olfactory activation of the human orbitofrontal cortex. Neuroreport 11, 893897.
26. Savic, I, Gulyas, B, Larsson, M et al. (2000) Olfactory functions are mediated by parallel and hierarchical processing. Neuron 26, 735745.
27. Yousem, DM, Williams, SCR, Howard, RO et al. (1997) Functional MR imaging during odor stimulation: preliminary data. Radiology 204, 833838.
28. Fulbright, RK, Skudlarski, P, Lacadie, CM et al. (1998) Functional MR imaging of regional brain responses to pleasant and unpleasant odors. AJNR Am J Neuroradiol 19, 17211726.
29. Weismann, M, Yousry, I, Heuberger, E et al. (2001) Functional magnetic resonance imaging of human olfaction. Neuroimaging Clin N Am 11, 237250.
30. Poellinger, A, Thomas, R, Lio, P et al. (2001) Activation and habituation in olfaction—an fMRI study. NeuroImage 13.
31. Cerf-Ducastel, B, Van de Moortele, PF, MacLeod, P et al. (2001) Interaction of gustatory and lingual somatosensory perceptions at the cortical level in the human: a functional magnetic resonance imaging study. Chem Senses 26, 371383.
32. Rudenga, K, Green, BG, Nachtigal, D et al. (2010) Evidence for an intgrated oral sensory module in the human ventral insula. . Chem Senses 35, 693703.
33. Guest, S, Grabenhorst, F, Essick, G et al. (2007) Human cortical representation of oral temperature. Physiol Behav 92, 975984.
34. Gilbertson, TA (1998) Gustatory mechanisms for the detection of fat. Curr Opin Neurobiol 8, 447452.
35. Mattes, RD (2005) Fat taste and lipid metabolism in humans. Physiol Behav 86, 691697.
36. de Araujo, IE & Rolls, ET (2004) Representation in the human brain of food texture and oral fat. J Neurosci 24, 30863093.
37. De Celis Alonso, B, Marciani, L, Head, K et al. (2007) Functional Magnetic Resonance imaging assessment of the cortical representation of oral viscosity. J Texture Stud 38, 725737.
38. Eldeghaidy, S, Marciani, L, McGlone, F et al. (2011) The cortical response to the oral perception of fat emulsions and the effect of taster status. J Neurophysiol 105, 25722581.
39. Grabenhorst, F, Rolls, ET, Parris, BA et al. (2010) How the brain represents the reward value of fat in the mouth. Cereb Cortex 20, 10821091.
40. Frank, S, Linder, K, Kullmann, S et al. (2012) Fat intake modulates cerebral blood flow in homeostatic and gustatory brain areas in humans. Am J Clin Nutr 95, 13421349.
41. Grabenhorst, F & Rolls, ET (2014) The representation of oral fat texture in the human somatosensory cortex. Hum Brain Mapp 35, 25212530.
42. Small, DM (2008) Flavor and the formation of category-specific processing in olfaction. Chem Percept 1, 136146.
43. Small, DM, Voss, J, Mak, YE et al. (2004) Experience-dependent neural integration of taste and smell in the human brain. J Neurophysiol 92, 18921903.
44. De Araujo, IET, Rolls, ET, Kringelbach, ML et al. (2003) Taste-olfactory convergence, and the representation of the pleasantness of flavour, in the human brain. Eur J Neurosci 18, 20592068.
45. Eldeghaidy, S, Marciani, L, Pfeiffer, J et al. (2011) Use of an immediate swallow protocol to assess taste and aroma integration in fMRI studies. Chemosens Percept 4, 163174.
46. Small, DM & Prescott, J (2005) Odor/taste integration and the perception of flavor. Exp Brain Res 166, 345357.
47. McCabe, C & Rolls, ET (2007) Umami: a delicious flavor formed by convergence of taste and olfactory pathways in the human brain. Eur J Neurosci 25, 18551864.
48. Verhagen, JV & Engelen, L (2006) The neurocognitive bases of human multimodal food perception: sensory integration. Neurosci Biobehav Rev 30, 613665.
49. Bartoshuk, LM, Duffy, VB & Miller, IJ (1994) PTC/PROP tasting: anatomy, psychophysics, and sex effects. Physiol Behav 56, 11651171.
50. Bartoshuk, LM, Duffy, VB, Fast, K et al. (2003) Labeled scales (eg, category, Likert, VAS) and invalid across-group comparisons: what we have learned from genetic variation in taste. Food Qual Prefer 14, 125138.
51. Chang, WI, Chung, JW, Kim, YK et al. (2006) The relationship between phenylthiocarbamide (PTC) and 6-n-propylthiouracil (PROP) taster status and taste thresholds for sucrose and quinine. Arch Oral Biol 51, 427432.
52. Miller, IJ & Reedy, FE (1990) Variations in human taste bud density and taste Intensity perception. Physiol Behav 47, 12131219.
53. Duffy, VB, Bartoshuk, LM, Lucchina, LA et al. (1996) Supertasters of PROP (6-npropylthiouracil) rate the highest creaminess to high-fat milk products. Chem Senses 21, 598.
54. Duffy, VB, Peterson, J & Bartoshuk, L (2004) Associations between taste genetics, oral sensation and alcohol intake. Physiol Behav 82, 435445.
55. Manrique, S & Zald, DH (2006) Individual differences in oral thermosensation. Physiol Behav 88, 417424.
56. Duffy, VB, Davidson, AC, Kidd, JR et al. (2004) Bitter receptor gene (TAS2R38), 6-n-propylthlouracll (PROP) bitterness and alcohol intake. Alcohol Clin Exp Res 28, 16291637.
57. Duffy, BV & Bartoshuk, LM (2000) Food acceptance and genetic variation in taste. J Am Diet Assoc 100, 647655.
58. Essick, GK, Chopra, A, Guest, S et al. (2003) Lingual tactile acuity, taste perception, and the density and diameter of fungiform papillae in female subjects. Physiol Behav 80, 289302.
59. Cruz, A & Green, BG (2000) Thermal stimulation of taste. Nature 403, 889892.
60. Green, BG & George, P (2004) Thermal taste’ predicts higher responsiveness to chemical taste and flavor. Chem Senses 29, 617628.
61. Bajec, MR & Pickering, G (2008) Thermal taste, PROP responsiveness, and perception of oral sensations. Physiol Behav 95, 581590.
62. Clark, R, Francis, S, Bealin-Kelly, F et al. (2011) The cortical response to carbonation and its interaction with taste perception. In 9th Pangborn Sensory Science Symp.
63. Stice, E, Spoor, S, Bohon, C et al. (2008) Relation of reward from food intake and anticipated food intake to obesity: a functional magnetic resonance imaging study. J Abnorm Psychol 117, 924935.
64. Felsted, JA, Ren, X, Chouinard-Decorte, F et al. (2010) Genetically determined differences in brain response to a primary food reward. J Neurosci 30, 24282432.
65. Wilcox, CE, Claus, ED, Blaine, SK et al. (2013) Genetic variation in the alpha synuclein gene (SNCA) is associated with BOLD response to alcohol cues. J Stud Alcohol Drugs 74, 233244.
66. Fuhrer, D, Zysset, S & Stumvoll, M (2008) Brain activity in hunger and satiety: an exploratory visually stimulated FMRI study. Obesity (Silver Spring) 16, 945950.
67. LaBar, KS, Gitelman, DR, Parrish, TB et al. (2001) Hunger selectively modulates corticolimbic activation to food stimuli in humans. Behav Neurosci 115, 493500.
68. Mohanty, A, Gitelman, DR, Small, DM et al. (2008) The spatial attention network interacts with limbic and monoaminergic systems to modulate motivation- induced attention shifts. Cereb Cortex 18, 26042613.
69. van der Laan, LN, de Ridder, DTD, Viergever, MA et al. (2011) The first taste is always with the eyes: a meta-analysis on the neural correlates of processing visual food cues. NeuroImage 55, 296303.
70. Killgore, WD, Young, AD, Femia, LA et al. (2003) Cortical and limbic activation during viewing of high- versus low-calorie food. Neuroimage 19, 13811394.
71. Goldstone, AP, de Hernandez, CG, Beaver, JD et al. (2009) Fasting biases brain reward systems towards high-calorie foods. Eur J Neurosci 30, 16251635.
72. Smeets, P, Graaf, C, Stafleu, A et al. (2006) Effect of satiety on brain activation during chocolate tasting in men and women. Am J Clin Nutr 83, 12971305.
73. Smeets, PAM, Weijzen, P, de Graaf, C et al. (2011) Consumption of caloric and non-caloric versions of a soft drink differentially affects brain activation during tasting. Neuroimage 54, 13671367.
74. Small, D, Zatorre, R, Dagher, A et al. (2001) Changes in brain activity related to eating chocolate From pleasure to aversion. Brain 124, 17201733.
75. Tataranni, P & DelParigi, A (2003) Functional neuroimaging: a new generation of human brain studies in obesity research. Obes Rev 4, 229238.
76. Gautier, J, Del Parigi, A, Chen, K et al. (2001) Effect of satiation on brain activity in obese and lean women. Obes Res 9, 676684.
77. Thaler, JP & Schwartz, MW (2010) Minireview: inflammation and obesity pathogenesis: the hypothalamus heats up. Endocrinology 151, 41094115.
78. Smeets, P, de Graaf, C, Stafleu, A et al. (2005) Functional MRI of human hypothalamic responses following glucose ingestion. Neuroimage 24, 363368.
79. Page, KA, Chan, O, Arora, J et al. (2013) Effects of fructose vs glucose on regional cerebral blood flow in brain regions involved with appetite and reward pathways. J Am Med Assoc 309, 6370.
80. von Ruesten, A, Steffen, A, Floegel, A et al. (2011) Trend in obesity prevalence in European adult cohort populations during follow-up since 1996 and their predictions to 2015. PLoS ONE 11, e27455.
81. Batterham, R, Ffytche, D, Rosenthal, J et al. (2007) PYY modulation of cortical and hypothalamic brain areas predicts feeding behaviour in humans. Nature 450, 106109.
82. De Silva, A, Salem, V, Long, CJ et al. (2011) The gut hormones PYY 3–36 and GLP-1 7–36 amide reduce food intake and modulate brain activity in appetite centers in humans. Cell Metab 14, 700706.
83. Moran, TH & Kinzig, KP (2004) Gastrointestinal satiety signals II. Cholecystokinin. Am J Physiol Gastrointest Liver Physiol 286, G183G188.
84. Liddle, RA, Goldfine, ID, Rosen, MS et al. (1985) Cholecystokinin bioactivity in human plasma. Molecular forms, responses to feeding, and relationship to gallbladder contraction. J Clin Invest 75, 11441152.
85. Batterham, R, Cowley, M, Small, C et al. (2002) Gut hormone PYY3–36 physiologically inhibits food intake. Nature 418, 650654.
86. Degen, L, Matzinger, D, Drewe, J et al. (2001) The effect of cholecystokinin in controlling appetite and food intake in humans. Peptides 22, 12651269.
87. Petrovich, G, Setlow, B, Holland, P et al. (2002) Amygdalo-hypothalamic circuit allows learned cues to override satiety and promote eating. J Neurosci 22, 87488753.
88. Lassman, D, Mckie, S, Gregory, L et al. (2010) Defining the role of cholecystokinin in the lipid-induced human brain activation matrix. Gastroenterology 138, 15141524.
89. Li, J, An, R, Zhang, Y et al. (2012) Correlations of macronutrient-induced functional magnetic resonance imaging signal changes in human brain and gut hormone responses. Am J Clin Nutr 96, 275282.
90. Eldeghaidy, S, Marciani, L, Hort, J et al. (2014) Prior feeding of fat modulates the cortical response to fat in the mouth in humans. In Joint Annual Metting ISMRM-ESMRMB.
91. Sun, X, Veldhuizenb, MG, Wray, AE et al. (2014) The neural signature of satiation is associated with ghrelin response and triglyceride metabolism. Physiol Behav.
92. Goldstone, AP, Prechtl, CG, Scholtz, S et al. (2014) Ghrelin mimics fasting to enhance human hedonic, orbitofrontal cortex, and hippocampal responses to food. Am J Clin Nutr 99, 13191330.
93. Malik, S, McGlone, F, Bedrossian, D et al. (2008) Ghrelin modulates brain activity in areas that control appetitive behavior. Cell Metab 7, 400409.
94. Szalay, C, Aradi, M, Schwarcz, A et al. (2012) Gustatory perception alterations in obesity: an fMRI study. Brain Res 1473, 131140.
95. Rothemund, Y, Preuschhof, C, Bohner, G et al. (2007) Differential activation of the dorsal striatum by high-calorie visual food stimuli in obese individuals. Neuroimage 37, 410421.
96. Stoeckel, LE, Weller, RE, Cook, EW et al. (2008) Widespread reward-system activation in obese women in response to pictures of high-calorie foods. Neuroimage 41, 636647.
97. Stoeckel, LE, Kim, J, Weller, RE et al. (2009) Effective connectivity of a reward network in obese women. Brain Res Bull 79, 388395.


Imaging methodologies and applications for nutrition research: what can functional MRI offer?

  • Susan T. Francis (a1) and Sally Eldeghaidy (a1) (a2)
  • Please note a correction has been issued for this article.


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