Skip to main content Accessibility help
×
Home

Contents:

Information:

  • Access
  • Cited by 1

Actions:

      • Send article to Kindle

        To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

        Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

        Find out more about the Kindle Personal Document Service.

        Flavonoid-rich-berry-extract treatment decreases the expression of DMT1 and functionally-similar metal transporter genes in human intestinal Caco-2 cells
        Available formats
        ×

        Send article to Dropbox

        To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

        Flavonoid-rich-berry-extract treatment decreases the expression of DMT1 and functionally-similar metal transporter genes in human intestinal Caco-2 cells
        Available formats
        ×

        Send article to Google Drive

        To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

        Flavonoid-rich-berry-extract treatment decreases the expression of DMT1 and functionally-similar metal transporter genes in human intestinal Caco-2 cells
        Available formats
        ×
Export citation

Berries are a rich dietary source of bioactive polyphenols including flavonoids such as anthocyanins(1). The ability of flavonoids to chelate divalent metal ions may contribute to their antioxidant action. Dietary polyphenols are known to impair Fe absorption. They can reduce non-haem-Fe transport across Caco-2 cell monolayers(2). However, little is known about their influence on the expression of the genes involved. The present study investigated the influence of berry polyphenols on the expression of metal transporter and related genes.

Human intestinal Caco-2 cells were cultured for 21 d and were then treated for 16 h with an anthocyanin-rich berry extract (OptiBerry; InterHealth Nutraceuticals, Benicia, CA, USA) at a final concentration of 0.5% (w/v). Subsequently, mRNA was extracted, pooled and used for the microarray analysis using Affymetrix gene chip HG-U133A (Affymetrix Inc., Santa Clara, CA, USA) and for quantitative RT–PCR. Microarray data were analysed using GCOS™ software (Affymetrix Inc.) and the Database for Annotation, Visualisation and Integrated Discovery (version 4; Laboratory of Immunopathogenesis and Bioinformatics, National Cancer Institute at Frederick, Frederick, MD, USA). A cut-off of 2-fold change and P⩽0.05 was applied.

DMT1 (divalent metal transporter; cellular Fe absorption), ZIP2 (Zn uptake transporter), ZNT10 (Zn efflux transporter), ATP7B (Cu efflux transporter), MRS2 (Mg uptake transporter) and HFE (regulation of Fe absorption) were identified as a novel group of metal transporter and related genes affected by the treatment. Functional similarity of these genes to DMT1 was determined (Fig. 1). Expression of DMT1, ZNT10, ZIP2, HFE, ATP7B and MRS2 was decreased by the treatment (Fig. 2) and the microarray data was validated by quantitative RT–PCR using the 18S amplicon as a housekeeping gene (mean fold decreases 1.70 (sd 0.73), 1.74 (sd 0.56), 1.74 (sd 0.82), 2.43 (sd 0.72), 1.11 (sd 0.36) and 1.11 (sd 0.36) respectively).

Fig. 1. Functional similarity to DMT1 of metal transporter and related genes.

Fig. 2. Microarray fold-decrease in expression of metal transporter and related genes.

Studies are in progress to investigate the biological relevance of the observed effects in relation to berry consumption and the absorption of dietary Fe and Zn, Cu and Mg.

1. Zafra-Stone, S, Yasmin, T, Bagchi, M et al. (2007) Mol Nutr Food Res 51, 675683.
2. Kim, EY, Ham, SK, Shigenaga, MK et al. (2008) J Nutr 138, 16471651.