Skip to main content Accessibility help
×
Home

Contents:

Information:

  • Access

Actions:

      • Send article to Kindle

        To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

        Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

        Find out more about the Kindle Personal Document Service.

        Effect of high fat diet and moderate exercise on T-lymphocytes of Peyer's patches mice
        Available formats
        ×

        Send article to Dropbox

        To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

        Effect of high fat diet and moderate exercise on T-lymphocytes of Peyer's patches mice
        Available formats
        ×

        Send article to Google Drive

        To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

        Effect of high fat diet and moderate exercise on T-lymphocytes of Peyer's patches mice
        Available formats
        ×
Export citation

Moderate physical exercise alone has a positive influence on the immune system(1) but the effect of a high fat diet (↑LIPIDS)(2) combined with moderate physical exercise in young people has not been established; the objective of this study was to assess whether a high fat diet and moderate physical exercise modifies the T-lymphocyte (TLc) population of Peyer's patches (PP) of young Balb/c mice. Thirty-two 21-d-old male mice were divided into four groups, two groups swam for 30 min, 5 d per week during 9 weeks (E groups) and two groups were left without exercise; one of the exercise and one without exercise were fed a standard diet (CONTROL) and the other two groups were fed a high fat diet (↑LIPIDS) (DIO Rodent Purified Diet, Cat. 58V8; energy: 3.78 kcal/g (15.81552 kJ/d)). Small intestines were dissected, PP were cut, macerated, filtered and centrifuged to obtain the lymphocyte pellet, cells were stained with anti-CD3+, CD4+ and CD8+ antibodies. The diet only increased the weight of mice who made exercise (25.7, sd 0.0) compared with non-exercise group (21.5, sd 2.8) and the control group (20.2, sd 1.4), P<0.005. CD3+ TLc (25.6, sd 1.0), CD3+/CD4+ (20.8, sd 1.3) and CD3+/CD8+ (2.62, sd 0.3) decreased significantly in the group ↑LIPIDS/E (in comparison with CONTROL/E: 38.8, sd 2.5, t 13.87; 33.5, sd 2.0, t 15.01; 4.93, sd 0.5, t 10.96, respectively), P⩽0.001. Thus, the diet did not change the weight of the animals but significantly altered the amount of T-cells, this probably due to the high content of saturated fat in the diet (2.91% from soya oil; 20.69% from lard) (Fig. 1).

Fig. 1. Percentage of lymphocytes CD3+, CD3+/CD4+ and CD3+/CD8+ from PP of Balb/c mice, feeding with standard diet (CONTROL) or high fat diet (↑LIPIDS); with 30 min exercise (/E), or without it. Values are show in means (sd) (n 8), differences were statistically significant by Student's t test at P⩽0.001. CONTROL: group with standard diet without exercise; ↑LIPIDS: group with high fat diet; CONTROL/E: group with standard diet with exercise; ↑LIPIDS/E: group feeding with high fat diet and with exercise.

This project was financed by Universidad Autónoma del Estado de México.

1.Pedersen, BK & Hoffman-Goetz, L (2000) Physiol Rev 80, 10551081.
2.Kaminogawa, S & Nanno, M (2004) Altern Med 1, 241250.