Skip to main content Accessibility help
×
Home

An overview of methods for assessment of free radical activity in biology

  • Malcolm J. Jackson (a1)

Abstract

Assays which purport to assess free radical activity in biological systems are multiple. However, despite numerous published descriptions of new methods and modifications of methods to assess free radical activity in biological materials, there is still a lack of reliable techniques for quantification of activity in vivo. Analysis of a number of related indicators and use of a variety of approaches appears the only reliable way to evaluate these processes in vivo. In studies of free radical generation by contracting skeletal muscle we have attempted to use a variety of indicators, including measurement of endogenous antioxidant levels, measurement of indirect indicators of free radical activity (e.g. products of lipid peroxidation, DNA oxidation or protein oxidation) and, where possible, measurement of direct indicators of free radical activity by electron spin resonance techniques. In view of the relative lack of specificity of many available techniques, caution should be exerted in evaluating the numerous examples of isolated single measures of free radical activity which are present in the scientific literature.

    • Send article to Kindle

      To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      An overview of methods for assessment of free radical activity in biology
      Available formats
      ×

      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

      An overview of methods for assessment of free radical activity in biology
      Available formats
      ×

      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

      An overview of methods for assessment of free radical activity in biology
      Available formats
      ×

Copyright

Corresponding author

Corresponding Author: Professor Malcolm J. Jackson, fax +44 (0)151 706 5952, email mjj@liv.ac.uk

References

Hide All
Ashton, T, Rowlands, CC, Jones, E, Young, IS, Jackson, SK, Davies, B & Peters, JR (1998) Electron spin resonance spectroscopic detection of oxygen-centred radicals in human serum following exhaustive exercise. European Journal of Applied Physiology 77, 498502.
Beckley, RJ (1976) Free radicals in dry biological systems. In Free Radicals in Biology, Vol. 2, pp. 135146 [Pryor, WA, editor]. New York: Academic Press.
Dillard, CJ, Litov, RE, Savin, WM, Dunelin, EE & Tappel, AL (1978) Effects of exercise, vitamin E and ozone on pulmonary function and lipid peroxidation. Journal of Applied Physiology 45, 927932.
Dizdaroglu, M (1991) Chemical determination of free radical-induced damage to DNA. Free Radical Biology and Medicine 10, 225230.
Dormandy, TL & Wickens, DG (1987) The experimental and clinical pathology of diene conjugation. Chemistry and Physics of Lipids 45, 353364.
Evans, PJ & Halliwell, B (1990) Measurement of iron and copper in biological systems: bleomycin and copper-phenanthroline assays. Methods in Enzymology 233, 8292.
Forman, A & Borg, DC (1989) Electron spin resonance in biology. In CRC Handbook of Free Radicals and Antioxidants in Biomedicine, Vol. 3, pp. 167186 [Miguel, J, Quintanilha, AT and Weber, H, editors]. Boca Raton, FL: CRC Press.
Garlick, PB, Davies, MJ, Hearse, DJ & Slater, TF (1987) Direct detection of free radicals in the reperfused rat heart using electron spin resonance spectroscopy. Circulation Research 61, 757760.
Gibson, R (1990) Principles of Nutritional Assessment. Oxford: Oxford University Press.
Grech, ED, Dodd, NJF, Jackson, MJ, Morrison, WL, Faragher, EB & Ramsdale, DR (1996) Evidence for free radical generation after primary percutaneous transluminal coronary angioplasty recanalization in acute myocardial infarction. American Journal of Cardiology 77, 122127.
Grootveld, M & Halliwell, B (1986) Aromatic hydroxylation as a potential measure of hydroxyl radical formation in vivo. Identification of hydroxylated derivatives of salicylate in human body fluids. Biochemical Journal 237, 499504.
Grootveld, M & Halliwell, B (1987) Measurement of allantoin and uric acid in human body fluids. A potential index of free radical reactions in vivo. Biochemical Journal 243, 803808.
Halliwell, B & Aruoma, OI (1991) DNA damage by oxygen-derived species: its mechanism, and measurement in mammalian systems. FEBS Letters 281, 919.
Jack, CIA, Ridgeway, E, Jackson, MJ & Hind, CRK (1994) Serum octa-9,11 dienoic acid – An assay of free radical activity or as a result of bacterial production? Clinica Chimica Acta 224, 139146.
Jackson, MJ (1994) Can dietary micronutrients influence tissue antioxidant capacity? Proceedings of the Nutrition Society 55, 5357.
Jackson, MJ, Brooke, MH, Kaiser, K & Edwards, RHT (1991) Glutathione depletion during experimental damage to rat skeletal muscle and its relevance to Duchenne muscular dystrophy. Clinical Science 80, 559564.
Jackson, MJ & Johnson, K (1989) Application of electron spin resonance techniques to the detection of free radicals in muscle tissue. In CRC Handbook of Free Radicals and Antioxidants in Biomedicine, Vol. 3, pp. 209214 [Miquel, J, Quintanilha, AT and Weber, H, editors]. Boca Raton, FL: CRC Press.
Kaur, H & Halliwell, B (1996) Salicylic acid and phenylalanine as probes to detect hydroxyl radicals. In Free Radicals: A Practical Approach, pp. 101118 [N, Punchard and FJ, Kelly, editors]. Oxford: Oxford University Press.
Levine, RL, Williams, JA, Stadman, ER & Shacter, E (1994) Carbonyl assays for determination of oxidatively modified proteins. Methods in Enzymology 233, 346359.
Meister, A & Anderson, ME (1983) Glutathione. Annual Review of Biochemistry 52, 711760.
Miller, RM, Sies, H, Park, EM & Thomas, JA (1990) Phosphorylase and creatine kinase modification by thiol disulphide exchange and by xanthine oxidase-initiated S-thiolation. Archives of Biochemistry and Biophysics 276, 355363.
Podmore, ID, Griffiths, HR, Herbert, KE, Mistry, N, Mistry, P & Lunec, J (1998) Vitamin C exhibits pro-oxidant properties. Nature 392, 559.
Rangan, U & Bulckley, GB (1993) Prospects for treatment of free radical-mediated tissue injury. British Medical Bulletin 479, 700718.
Shigenaga, MK, Aboujaoude, EN, Chen, Q & Ames, BN (1994) Assays of oxidative DNA damage biomarker, 8-oxo-2'-deoxyguanosine and 8-oxoguanine in nuclear DNA and biological fluids by high performance liquid chromatography with electrochemical detection. Methods in Enzymology 234, 1632.
Snyder, MT, Balke, PO & Oertes, KE (1987) Lipid peroxidation during muscular exercise in man: inferences from the pulmonary excretion of n-pentane, isopentane and nitrogen. Proceedings of the Nutrition Society 45, 64A.
Symons, MCR (1978) Chemical and Biochemical Aspects of Electron Spin Resonance. London: Van Nostrand-Reinhold.
Yagi, K (1989) A simple fluorimetric assay for lipid peroxides in blood serum or plasma. In CRC Handbook of Free Radicals and Antioxidants in Biomedicine, Vol. 3, pp. 215218 [Miguel, J, Quintanilha, AT and Weber, H, editors]. Boca Raton, FL: CRC Press.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed