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New Saccharum hybrids in S. spontaneum cytoplasm developed through a combination of conventional and molecular breeding approaches

Published online by Cambridge University Press:  16 October 2024

Y.-B. Pan ,
D. M. Bumer ,
Q. Wei ,
G. M. Cordeiro ,
B. L. Legendre  and
R. J. Henry
Y.-B. Pan*
Affiliation:
1USDA-ARS, Southern Regional Research Center, Sugarcane Research Unit, 5883 USDA Road, Houma, LA 70360, USA
D. M. Bumer
Affiliation:
1USDA-ARS, Southern Regional Research Center, Sugarcane Research Unit, 5883 USDA Road, Houma, LA 70360, USA
Q. Wei
Affiliation:
1USDA-ARS, Southern Regional Research Center, Sugarcane Research Unit, 5883 USDA Road, Houma, LA 70360, USA
G. M. Cordeiro
Affiliation:
2Centre for Plant Conservation Genetics; Southern Cross University, PO. Box 157, Lismore, NSW 2480, Australia
B. L. Legendre
Affiliation:
1USDA-ARS, Southern Regional Research Center, Sugarcane Research Unit, 5883 USDA Road, Houma, LA 70360, USA
R. J. Henry
Affiliation:
2Centre for Plant Conservation Genetics; Southern Cross University, PO. Box 157, Lismore, NSW 2480, Australia
*
* Corresponding author. E-mail: ypan@srrc.ars.usda.gov
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Abstract

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Identification of sugarcane hybrids is difficult when selections are based solely on morphological traits. Our objective was to combine morphological traits and molecular marker analysis to select F1 hybrids from two separate crosses between Djatiroto, a clone of Saccharum spontaneum, and elite sugarcane clones, LCP 85-384 (Cross 97-3144) and CP 62-258 (Cross 97-3146). The maternal inflorescences of Djatiroto were emasculated by submersion in a circulating 45°C hot-water tank for 10 min to minimize self-fertilization. Cross 97-3144 produced 4.7 g of seeds with 338 viable seeds per gram and Cross 97-3146 produced 2.4 g of seeds with 166 viable seeds per gram. After greenhouse germination, 96 progeny from each cross were evaluated in a field plot. Evaluations were conducted on the ratoon crops for stalk diameter (mm), juice Brix (percentage soluble solids), and a randomly amplified polymorphic DNA (RAPD) marker OPA-11-366 that was reproducibly amplified through PCR from the elite clones, but not the maternal S. spontaneum clone. Fifty progeny (52.1%) from Cross 97-3144 and 36 progeny (37.5%) from Cross 97-3146 inherited the RAPD marker. Five putative F1 progeny were selected from each cross, namely US 99-43, US 99-44, US 99-45, US 99-46 and US 99-47 from Cross 97-3144, and US 99-48, US 99-49, US 99-50, US 99-51 and US 99-52 from Cross 97-3146, based on their relatively larger stalk diameter, higher Brix and inheritance of the RAPD marker. The hybrid nature of these selected progeny was verified with sugarcane microsatellite markers. This is the first report of the development of Saccharum hybrids with the cytoplasm of S. spontaneum for breeding purpose through a combination of conventional and molecular breeding approaches. Availability of these F1 hybrids could enhance the genetic diversity of Saccharum germplasm and has enabled sugarcane geneticists and breeders to explore the possible contribution of S. spontaneum cytoplasm in the development of new sugarcane cultivars.

Keywords

microsatelliteRAPDSaccharum spontaneum cytoplasmsugarcane
Type
Research Article
Information
Plant Genetic Resources , Volume 2 , Issue 2 , August 2004 , pp. 131 - 139
Copyright
© NIAB 2004

Footnotes

†

USDA-ARS, Dale Bumpers Small Farms Research Center, 6883 S State Hwy 23, Booneville, AR 72927-9214, USA.

‡

LSU Ag Center Research & Extension, Sugar Research Station, 5755 LSU Ag Road, St. Gabriel, LA 70776, USA. Disclaimer: Product names and trademarks are mentioned to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA does not imply the approval of the product to the exclusion of others that may also be suitable. The experiments reported comply with the current laws of the USA.

References

Al-Janabi, SM, McClelland, M, Petersen, C and Sobral, BWS (1994) Phylogenetic analysis of organellar DNA sequences in the Andropogoneae: Saccharinae. Theoretical and Applied Genetics 88: 933–944.CrossRefGoogle Scholar
Arceneaux, G (1967) Cultivated sugarcanes of the world and their botanical derivation. Proceedings of the International Society for Sugar Cane Technology 12: 844–854.Google Scholar
Artschwager, E and Brandes, EW (1958) Sugarcane (Saccharum officinarum L): origin, classification, characteristics and descriptions of representative clones. USD A Handbook No. 122.Google Scholar
Besse, P, McIntyre, CL and Berding, N (1996) Ribosomal DNA variations in Erianthus, a wild sugarcane relative (Andropogoneae-Saccharinae). Theoretical and Applied Genetics 92: 733–743.CrossRefGoogle ScholarPubMed
Besse, P, Taylor, G, Carrol, B, Berding, N, Burner, D and McIntyre, CL (1998) Assessing genetic diversity in a sugarcane germ- plasm collection using an automated AFLP analysis. Genetica 104: 143–153.CrossRefGoogle Scholar
Burner, DM (1997) Chromosome transmission and meiotic behavior in various sugarcane crosses. Journal of the American Society for Sugar Cane Technology 17: 38–50.Google Scholar
Burner, DM and Legendre, BL (1993a) Chromosome transmission and meiotic stability of sugarcane (Saccharum spp.) hybrid derivatives. Crop Science 33: 600–606.CrossRefGoogle Scholar
Burner, DM and Legendre, BL (1993b) Sugarcane genome amplification for the subtropics: a twenty year effort. Sugar Cane 1993(3): 5–10.Google Scholar
Burner, DM, Pan, Y-B and Webster, RD (1997) Genetic diversity of North American and Old World Saccharum assessed by RAPD analysis. Genetic Resources and Crop Evolution 44: 235–240.CrossRefGoogle Scholar
Chu, TL, Juang, PY and Shang, KC (1962) The wild cane (S. spontaneum) in Taiwan. Report of the Taiwan Experimental Station 28: 1–11.Google Scholar
Cordeiro, GM, Taylor, GO and Henry, RJ (2000) Characterisation of microsatellite markers from sugarcane (Saccharum sp.), a highly polyploid species. Plant Science 155: 161–168.CrossRefGoogle Scholar
Cordeiro, GM, Casu, R, McIntyre, CL, Manners, JM and Henry, RJ (1999) Microsatellite markers from sugarcane (Saccharum sp.) ESTs transferable to Erianthus and sorghum. Plant Science 160: 1115–1123.CrossRefGoogle Scholar
Cordeiro, GM, Pan, Y-B and Henry, RJ (2003) Sugarcane microsatellites for the assessment of genetic diversity in sugarcane germplasm. Plant Science 165: 181–189.CrossRefGoogle Scholar
da Silva, JAG (2001) Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs). Genetics and Molecular Biology 24(1-4): 155–159.CrossRefGoogle Scholar
D'Hont, A, Lu, Y-H, Feldmann, P and Glaszmann, JC (1993) Cytoplasmic diversity in sugarcane revealed by heterologous probes. Sugar Cane 1993(1): 12–15.Google Scholar
D'Hont, A, Lu, Y-H, de León, DG, Grivet, L, Feldmann, P, Lanaud, C and Glaszmann, JC (1994) A molecular approach to unraveling the genetics of sugarcane, a complex polyploid of the Andropogoneae tribe. Genome 37: 222–230.CrossRefGoogle ScholarPubMed
D'Hont, A, Rao, PS, Feldmann, P, Grivet, L, Islam-Faridi, N, Taylor, P and Glaszmann, JC (1995) Identification and characterisation of sugarcane intergeneric hybrids, Saccharum officinarum X Erianthus arundinaceus, with molecular markers and DNA in situ hybridisation. Theoretical and Applied Genetics 91: 320–326.CrossRefGoogle Scholar
Dunckelman, PH and Breaux, RD (1969) Agronomic characteristics of Saccharum spontaneum in culture in Houma, Louisiana. International Sugar Journal 71: 333–334.Google Scholar
Dunckelman, PH and Legendre, BL (1982) Guide to Sugarcane Breeding in the Temperate Zone. New Orleans: USDA- ARS, ARM-S-22.Google Scholar
Gill, BS and Grassi, CO (1986) Pathways of genetic transfer in intergeneric hybrids of sugar cane. Sugar Cane 1986(2): 2–7.Google Scholar
Grassi, CO (1946) Saccharum robustum and other wild relatives of ‘noble’ sugar canes. Journal of the Arnold Arboretum 27: 234–252.CrossRefGoogle Scholar
Grassi, CO (1969) Saccharum names and their interpretation. Proceedings of the International Society of Sugar Cane Technologists 13: 868–875.Google Scholar
Harvey, M and Botha, FC (1996) Use of PCR-based methodologies for the determination of DNA diversity between Saccharum varieties. Euphytica 89: 257–265.CrossRefGoogle Scholar
Harvey, M, Huckett, BI and Botha, FC (1994) Use of polymerase chain reaction (PCR) and random amplification of polymorphic DNAs (RAPDs) for the determination of genetic distances between 21 sugarcane varieties. Proceedings of the South African Sugar Technology Association 68: 36–40.Google Scholar
Hoarau, JY, Offmann, B, D'Hont, A, Risterucci, AM, Roques, D, Glaszmann, JC and Grivet, L (2001) Genetic dissection of a modern sugarcane cultivar (Saccharum spp.). I. Genome mapping with AFLP markers. Theoretical and Applied Genetics 103: 84–97.CrossRefGoogle Scholar
Huckett, BI and Botha, FC (1995) Stability and potential use of RAPD markers in a sugarcane genealogy. Euphytica 86: 117–125.CrossRefGoogle Scholar
Kandasami, PA, Sreenivasan, TV, Palanichami, K and Ramana Rao, TC (1983) Sugarcane germplasm: classification of clones. Sugar Cane 1983(2): 1–3.Google Scholar
Lanham, PG, Fennell, S, Moss, JP and Powell, W (1992) Detection of polymorphic loci in Arachis germplasm using random amplified polymorphic DNAs. Genome 35: 885–889.CrossRefGoogle ScholarPubMed
Legendre, BL (1992) The core/press method for predicting the sugar yield from cane for use in cane payment. Sugar Journal 54(9): 2–7.Google Scholar
Linnaeus, C (1753) Species Plantarum, 2 volumes. Stockholm (1959 facsimile edition, London: Ray Society).Google Scholar
Linnaeus, C (1771) Mantissa Plantarum Altera. Stockholm (1961 facsimile edition, Weinheim: J. Cramer, p. 183).Google Scholar
Machado, GR Jr, Walker, DI, Bressiani, JA and da Silva, JAG (1995) Emasculation of sugarcane tassels using hot water. Proceedings of the International Society for Sugar Cane Technology 22: 346–351.Google Scholar
Marshall, P, Marchand, M-C, Lisieczko, Z and Landry, BS (1994) A simple method to estimate the percentage of hybridity in canola (Brassica napus) F1 hybrids. Theoretical and Applied Genetics 89: 853–858.CrossRefGoogle ScholarPubMed
McIntyre, L and Jackson, P (1995) Does selfing occur in sugarcane? Plant Animal Genome IV Conference, P165.Google Scholar
Melloto-Passarin, DM, Calsar-Junior, T and Carrer, H (2004) Complete chloroplast DNA sequence of sugarcane reveals similarity and diversity among Saccharum species, sorghum, maize, rice and wheat. Plant Genome XII Conference, W124.Google Scholar
Miller, JD (1977) Combining ability and yield component analyses in a five-parent diallel cross in sugarcane. Crop Science 17: 545–547.CrossRefGoogle Scholar
Miller, JD and Tai, PYP (1992) Use of plant introductions in sugarcane cultivar development. In: Shands, HL and Weisner, LE (eds) Use of Plant Introductions in Cultivar Development, Part 2. CSS A Special Publication 20. Madison, WI: CSSA, pp. 137–149.Google Scholar
Milligan, SB, Gravois, KA, Bischoff, KP and Martin, FA (1990) Crop effects on broad-sense heritabilities and genetic variances of sugarcane yield components. Crop Science 30: 344–349.CrossRefGoogle Scholar
Milligan, SB, Martin, FA, Bischoff, KP, Quebedeaux, JP, Dufrene, EO, Quebedeaux, KL, Hoy, JW, Reagan, TE, Legendre, BL and Miller, JD (1994) Registration of ‘LCP 85-384’ sugarcane. Crop Science 34: 819–820.CrossRefGoogle Scholar
Ming, R, Liu, SC, Bowers, JE, Moore, PH, Irvine, JE and Paterson, AH (2000) Construction of a Saccharum consensus genetic map from two interspecific crosses. Crop Science 42: 570–583.Google Scholar
Msomi, N and Botha, FC (1994) Identification of molecular markers linked to fibre using bulk segregant analysis. Proceedings of the South African Sugar Technology Association 68: 41–45.Google Scholar
Mudge, J, Andersen, WR, Kehrer, RL and Fairbanks, DJ (1996) A RAPD genetic map of Saccharum officinarum. Crop Science 36: 1362–1366.CrossRefGoogle Scholar
Nagai, C (1984) Emasculation of sugarcane tassels by hot-water treatment. Annual Report of the Experimental Station, Hawaii Sugar Plantation Association, pp. 3–4.Google Scholar
Nagatomi, S and Ohshiro, Y (1983) Classification of sugarcane wild germplasm by methods of numerical taxonomy. Proceedings of the International Society for Sugar Cane Technology 18: 650–660.Google Scholar
Pan, Y-B, Burner, DM, Ehrlich, KC, Grisham, MP and Wei, Q (1997) Analysis of primer-derived non-specific amplification products in RAPD-PCR. BioTechniques 22: 1071–1077.CrossRefGoogle Scholar
Pan, Y-B, Burner, DM and Legendre, BL (2000) An assessment of the phylogenetic relationship among sugarcane and related taxa based on the nucleotide sequence of 5S rRNA intergenic spacers. Genetica 108: 285–295.CrossRefGoogle ScholarPubMed
Pan, Y-B, Burner, DM and Wei, Q (2001) Developing species- specific DNA markers to assist in sugarcane breeding. Proceedings of the International Society for Sugar Cane Technology 24: 337–342.Google Scholar
Pan, Y-B, Miller, JD, Schnell, RJ, Richard, RP Jr and Wei, Q (2003b) Application of microsatellite and RAPD fingerprints in the Florida sugarcane variety program. Sugar Cane International, March/April: 19–28.Google Scholar
Pan, Y-B, Cordeiro, GM, Richard, EP Jr and Henry, RJ (2003a) Molecular genotyping of sugarcane clones with microsatellite DNA markers. Maydica 48: 319–329.Google Scholar
Pan, Y-B, Burner, DM, Legendre, BL, Grisham, MP and White, WH (2004) An assessment of the genetic diversity within a collection of Saccharum spontaneum with RAPD-PCR. Genetic Resources and Crop Evolution (in press).Google Scholar
Piperidis, G, Christopher, MJ, Carroll, BJ, Berding, N and D'Hont, A (2000) Molecular contribution to selection of intergeneric hybrids between sugarcane and the wild species Erianthus arundinaceus. Genome 43: 1033–1037.CrossRefGoogle ScholarPubMed
Price, S (1957) Cytological studies in Saccharum and allied genera. III. Chromosome numbers in interspecific hybrids. Botanist's Gazette 118: 146–159.CrossRefGoogle Scholar
Price, S (1960) Cytological studies in Saccharum and allied genera: VI. Chromosome numbers in S. officinarum and other noble sugar canes. Hawaii Plant Record 56: 183–194.Google Scholar
Rao, JT and Vijayalakshmi, U (1963). World Catalogue of Sugarcane Genetic Stock. Coimbatore, India: Sugarcane Breeding Institute (ICAR), pp. 1–77.Google Scholar
Sills, GR, Bridges, W, Al-Janabi, SM and Sobral, BWS (1995) Genetic analysis of agronomic traits in a cross between sugarcane (Saccharum officinarum L.) and its presumed progenitor (5. robustum Brandes, and Jesw. ex Grassi). Molecular Breeding 1: 355–363.CrossRefGoogle Scholar
Sobral, BWS and Honeycutt, RJ (1993) High output genetic mapping of polyploids using PCR-generated markers. Theoretical and Applied Genetics 86: 105–112.CrossRefGoogle ScholarPubMed
Sobral, BWS and Honeycutt, RJ (1994) Genetics, plants, and the polymerase chain reaction. In: Mullins, KB, Ferre, F and Gibbs, RA (eds) The Polymerase Chain Reaction. Boston, MA: Birkhauser, pp. 304–319.CrossRefGoogle Scholar
Sreenivasan, TV, Ahloowalia, BS and Heinz, DJ (1987) Cytogenetics. In: Heinz, DJ (ed.) Sugarcane Improvement Through Breeding. Amsterdam: Elsevier, pp. 211–253.CrossRefGoogle Scholar
Steel, RGD and Torne, JH (1980) Principles and Procedures of Statistics, a Biometrical Approach. New York: McGraw-Hill.Google Scholar
Tai, PYP (1989) Progress and problems of intergeneric hybridization in sugarcane breeding. Proceedings of the Inter-America Sugar Cane Seminar I: 391–395.Google Scholar
Tai, PYP, Miller, JD and Legendre, BL (1994) Preservation of Saccharum spontaneum germplasm through storage of true seeds. Sugar Cane 6: 3–8.Google Scholar
Tai, PYP, Miller, JD and Legendre, BL (1995) Evaluation of the world collection of Saccharum spontaneum. Proceedings of the International Society for Sugar Cane Technology 21: 250–260.Google Scholar
Welsh, J, Honeycutt, RJ, McClelland, M and Sobral, BWS (1991) Parentage determination in maize hybrids using the arbitrarily primer polymerase chain reaction (AP-PCR). Theoretical and Applied Genetics 82: 473–476.CrossRefGoogle Scholar