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Molecular analysis of five rice blast resistant genes in landraces from Myanmar and Laos

Published online by Cambridge University Press:  07 September 2020

Jiqiong Ma Open the ORCID record for Jiqiong Ma [Opens in a new window] ,
Yiding Sun ,
Yi Yang ,
Cuifeng Tang ,
Xinxiang A  and
Minghui Xu Open the ORCID record for Minghui Xu [Opens in a new window]
Jiqiong Ma
Affiliation:
Key Laboratory of Biotechnology Research of Yunnan Province, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture, Scientific Observation Station for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences Kunming, 650205, China
Yiding Sun
Affiliation:
Key Laboratory of Biotechnology Research of Yunnan Province, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture, Scientific Observation Station for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences Kunming, 650205, China
Yi Yang
Affiliation:
Key Laboratory of Biotechnology Research of Yunnan Province, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture, Scientific Observation Station for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences Kunming, 650205, China
Cuifeng Tang
Affiliation:
Key Laboratory of Biotechnology Research of Yunnan Province, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture, Scientific Observation Station for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences Kunming, 650205, China
Xinxiang A
Affiliation:
Key Laboratory of Biotechnology Research of Yunnan Province, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture, Scientific Observation Station for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences Kunming, 650205, China
Minghui Xu*
Affiliation:
Key Laboratory of Biotechnology Research of Yunnan Province, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture, Scientific Observation Station for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences Kunming, 650205, China
*
*Corresponding author. E-mail: ynxuminghui@163.com
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Abstract

In order to understand rice blast resistance gene patterns in rice landraces from Myanmar and Laos, we analysed the Pita, Pib, Pikh, Pi9 and Pi5 genes in 80 landraces through the use of functional molecular markers and functional fragment sequencing. These landraces were separated into four haplotypes (H1–H4) based upon three mutations identified in the Pita sequence. All four haplotypes were detected among landraces from Myanmar, whereas only the H1 and H2 haplotypes were detected among landraces from Laos. We additionally grouped landraces carrying 0–4 resistance genes into 18 genotypes based upon their resistance-susceptibility patterns and found that 16 of these genotypes were detected among Myanmar landraces at relative frequencies ranging from 2.50–12.50%, while 10 were detected among landraces from Laos at relative frequencies of 2.50–42.50%. The ‘Pib( + ) pikh(−) pi9(−) pi5(−) pita(−)’ genotype was found to be dominant, accounting for 12.5 and 42.5% of landraces from Myanmar and Laos, respectively. The Pib, Pikh, Pi5 and Pita genes were detected in 52.50, 32.50, 37.50 and 30.00% of landraces from Myanmar respectively, whereas they were detected in 52.50, 12.50, 30.00 and 17.50% of landraces from Laos, respectively. The Pi9 gene was rarely detected among analysed landraces and was particularly rare in those from Laos. These findings suggest that rice landraces from Myanmar are more genetically diverse than those from Laos, with clear differences in blast resistance gene patterns between landraces from these two countries.

Keywords

blast resistanceDNA markerlandraceMagnaporthe griseaOryza sativasequencing
Type
Research Article
Information
Plant Genetic Resources , Volume 18 , Issue 4 , August 2020 , pp. 287 - 293
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of NIAB

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