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In vivo grafting of wild Lens species to Vicia faba rootstocks

Published online by Cambridge University Press:  12 October 2011

Hai Ying Yuan ,
Monika Lulsdorf ,
Abebe Tullu ,
Valar Gurusamy  and
Albert Vandenberg
Hai Ying Yuan
Affiliation:
Crop Development Centre (CDC), University of Saskatchewan, 51 Campus Drive, Saskatoon, CanadaSK S7N 5A8
Monika Lulsdorf
Affiliation:
Crop Development Centre (CDC), University of Saskatchewan, 51 Campus Drive, Saskatoon, CanadaSK S7N 5A8
Abebe Tullu
Affiliation:
Crop Development Centre (CDC), University of Saskatchewan, 51 Campus Drive, Saskatoon, CanadaSK S7N 5A8
Valar Gurusamy
Affiliation:
Canadian Wheat Board, 310-15 Innovation Boulevard, Saskatoon, CanadaSK S7N 2X8
Albert Vandenberg*
Affiliation:
Crop Development Centre (CDC), University of Saskatchewan, 51 Campus Drive, Saskatoon, CanadaSK S7N 5A8
*
*Corresponding author. E-mail: bert.vandenberg@usask.ca
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Abstract

Faba bean (Vicia faba L.) was used as the rootstock for lentil scions to test the feasibility of using in vivo inter-generic grafting techniques as a substitute for root induction and as a tool in lentil genetic improvement. An accession of each of the six wild Lens species was used as the scion in grafts to faba bean breeding line FB50-9 rootstock. Successful grafts were obtained for all species with survival of grafts to seed maturity between 70.7 and 87.7% except for Lens orientalis PI 72735 with 55.3% survival. Days to flower remained the same after grafting, except for scions of L. nigricans PI 72560 and L. orientalis PI 72735 which had a lag phase of 9 and 7 d, respectively. For all six wild species, seed diameter and seed weight were not significantly different between non-grafted controls and scions grafted onto faba bean rootstocks. This simple approach opens the possibility of using in vivo grafting techniques to rescue inter-specific hybrids of lentil. The technique has potential as a useful tool in lentil breeding, as a means of improving seed multiplication rate of rare genetic resources of wild lentil and as a way to reduce the costs of germplasm multiplication of wild lentil species.

Keywords

genetic resourcesgraftingLensrootstockVicia faba L.
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 4 , December 2011 , pp. 543 - 548
Copyright
Copyright © NIAB 2011

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