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Correction of the misclassification of species in the Portuguese collection of Cucurbita pepo L. using DNA markers

Published online by Cambridge University Press:  16 July 2014

Ricardo Rodrigues ,
Iris Veiga ,
António Marreiros ,
Filomena Rocha  and
José Leitão
Ricardo Rodrigues
Affiliation:
Laboratory of Genomics and Genetic Improvement, FCT, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
Iris Veiga
Affiliation:
Laboratory of Genomics and Genetic Improvement, FCT, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
António Marreiros
Affiliation:
Direção Regional de Agricultura e Pescas do Algarve (DRAALG), Apartado 282, Patacão, 8001-904Faro, Portugal
Filomena Rocha
Affiliation:
Banco Português de Germoplasma Vegetal (BPGV)/INIAV,I.P., Quinta de S. José, S. Pedro de Merelim, 4700-859Braga, Portugal
José Leitão*
Affiliation:
Laboratory of Genomics and Genetic Improvement, FCT, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
*
* Corresponding author. E-mail: jleitao@ualg.pt
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Abstract

In this study, the genetic variability among 130 accessions of the Portuguese germplasm collection of Cucurbita pepo L. maintained at the Banco Português de Germoplasma Vegetal was assessed using AFLP (amplified fragment length polymorphism) and RAPD (random amplified polymorphic DNA) techniques for the identification of a genetically diverse core group of accessions for field phenotypic analysis. The surprisingly completely different molecular patterns exhibited by multiple accessions was later confirmed in the distribution of the putative C. pepo plants into two clusters drastically separated at a very low level of genetic similarity (DICE coefficient = 0.37). Additional analyses with RAPD and ISSR (inter single sequence repeat) markers and the introduction of standard genotypes of C. maxima L. and C. moschata L. into the analyses allowed the identification of multiple accessions of the last species wrongly included in the C. pepo collection. This study is a good example of the usefulness of DNA markers in the establishment and management of plant germplasm collections.

Keywords

CucurbitaceaeCucurbita pepoDNA markersplant genetic resources
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S160 - S163
Copyright
Copyright © NIAB 2014 

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