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Genetic diversity of Sinapis alba germplasm as revealed by AFLP markers

Published online by Cambridge University Press:  12 February 2007

Yong-Bi Fu*
Plant Gene Resources of Canada, Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, S7N 0X2, Canada
Richard K. Gugel
Plant Gene Resources of Canada, Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, S7N 0X2, Canada
Felicitas Katepa-Mupondwa
Plant Gene Resources of Canada, Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, S7N 0X2, Canada
*Corresponding author: E-mail:


Sinapis alba L. is a major specialty crop grown as a condiment in western Canada, but little is known about its genetic diversity. The objective of this study was to assess the level and pattern of genetic diversity in a collection of 127 S. alba accessions held at Plant Gene Resources of Canada using amplified fragment length polymorphism (AFLP) markers. Five AFLP primer pairs were applied, and 134 polymorphic bands were scored for each accession. These scored bands had frequencies of occurrence ranging from 0.02 to 0.99 with an average of 0.69. More AFLP variation was found within single (79.1%) than between (20.9%) S. alba accessions. A small degree of AFLP difference (1.7%) was observed among the accessions of various regions, while relatively large variation (9.2%) existed among the accessions of various countries. A large AFLP difference (15.6%) also existed between the yellow- and brown-seeded accessions, but only 6.2% difference was observed between the cultivar and landrace accessions. Two distinct groups of S. alba germplasm were identified on the basis of the seed colour (yellow or brown), although a few mixtures also existed. No apparent ‘duplicated’ accessions were observed. The most diverse accessions were from Italy, Spain, France and Greece. Among the most genetically distinct accessions were SA97 from Portugal, SA89 and SA88 from France, SA83 from Russia and SA57 from Italy. These findings are significant not only for managing S. alba germplasm, but also for identifying diverse germplasm that can be used by plant breeders to improve S. alba seed yield and quality parameters.

Research Article
Copyright © NIAB 2006

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Downey, RK and Rakow, G (1995) Mustard. In: Slinkard, AE, Knott, DR (eds) Harvest of Gold: The History of Field Crop Breeding in Canada. Saskatchewan: University of Saskatchewan, pp. 213219.Google Scholar
Excoffier, L, Smouse, PE and Quattro, JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131, 479491.Google Scholar
Fu, YB (2000) Effectiveness of bulking procedures in measuring population-pairwise similarity with dominant and co-dominant genetic markers. Theoretical and Applied Genetics 100, 12841289.CrossRefGoogle Scholar
Fu, YB, Diederichsen, A, Richards, KW and Peterson, G (2002) Genetic diversity within a range of cultivars and landraces of flax ( Linum usitatissimum L.) as revealed by RAPDs. Genetic Resources and Crop Evolution 49, 167174.CrossRefGoogle Scholar
Granot, D, Shabelsky, E, Schafferman, D and Yaniv, Z (1996) Analysis of genetic variability between populations of Sinapis alba and the effect of cultivation on the variability. Acta Horticulturae 407, 6774.Google Scholar
Huff, DR, Peakall, R and Smouse, PE (1993) RAPD variation within and among natural populations of outcrossing Buffalograss [Buchloe dactyloides (Nutt) Engelm.]. Theoretical and Applied Genetics 86, 927934.CrossRefGoogle ScholarPubMed
Huh, MK and Huh, HW (2001) AFLP fingerprinting of Brassica campestris L. ssp. napus var. nippo-oleifera Makino from Korea. Korean Journal of Biological Science 5, 101106.Google Scholar
Katepa-Mupondwa, F, Gugel, R, Raney, P and Rakow, G (2004). Genetic diversity among yellow mustard (Sinapis alba L.) accessions (abstract, P-111). In: Brassica 2004, Abstract Book, Joint Meeting of the 14th Crucifer Genetics Workshop and the 4th International Society for Horticultural Science Symposium on Brassicas, 2428 October, Daejeon, Korea, p. 218.Google Scholar
Lombard, V, Baril, CP, Dubreuil, P, Blouet, F and Zhang, D (2000) Genetic relationships and fingerprinting of rapeseed cultivars by AFLP: consequences for varietal registration. Crop Science 40, 14171425.CrossRefGoogle Scholar
Olsson, G (1960) Self-incompatibility and outcrossing in rape and white mustard. Hereditas 46, 241252.Google Scholar
Olsson, G (1974) Continuous selection for number of seeds per pod and oil content in white mustard. Hereditas 77, 197204.Google Scholar
Raney, P, Rakow, G and Olson, T (1995) Development of low erucic, low glucosinolate Sinapis alba. In: Proceedings of the 9th International Rapeseed Congress. Cambridge: Groupe Consultatif International de Recherche sur le Colza, pp. 416418.Google Scholar
Rohlf, FJ (1997) NTSYS-pc 2.1. Numerical Taxonomy and Multivariate Analysis System. Setauket, NY: Exeter Software.Google Scholar
Schneider, S, Roessli, D and Excoffier, L (2002). Arlequin ver 2.001: A Software for Population Genetics Data Analysis. Geneva: Genetics and Biometry Laboratory, University of Geneva, (accessed 30 March 2005).Google Scholar
Sokal, RR and Michener, CD (1958) A statistical method for evaluating systematic relationships. University of Kansas Science Bulletin 38, 14091438.Google Scholar
Srivastava, A, Gupta, V, Pental, D and Pradhan, AK (2001) AFLP-based genetic diversity assessment amongst agronomically important natural and some newly synthesized lines of Brassica juncea. Theoretical and Applied Genetics 102, 193199.CrossRefGoogle Scholar
Vos, P, Hogers, R, Bleeker, M, Reijans, M, van De, Lee T, Hornes, M, Frijters, A, Peleman, J, Kuiper, M and Zabeau, M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23, 44074414.Google Scholar
Williams, JKG, Kubelik, KR, Livak, KJ, Rafalski, JA and Tingey, SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 65316535.CrossRefGoogle ScholarPubMed
Yaniv, Z, Schafferman, D, Elber, Y, Ben-Moshe, E and Zur, M (1994) Evaluation of Sinapis alba, native to Israel, as a rich source of erucic acid in seed oil. Industrial Crops and Products 2, 137142.Google Scholar